CC BY-NC-ND 4.0 · SynOpen 2021; 05(04): 285-290
DOI: 10.1055/a-1647-7202
paper

Stereoselective Synthesis of the C19–C39 Fragment of Bastimolide A

Nemilikonda Sravan Kumar
a   Department of Organic Synthesis & Process Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad-500007, India
b   Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India
,
B. Janaki Ramulu
a   Department of Organic Synthesis & Process Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad-500007, India
,
Subhash Ghosh
a   Department of Organic Synthesis & Process Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad-500007, India
b   Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India
› Author Affiliations
N.S.K. thanks the University Grants Commission (UGC) New Delhi for a fellowship, and B.J.R. thanks the Science and Engineering Research Board (SERB) for a National Post Doctoral Fellowship (N-PDF).
 


Abstract

This paper describes the synthesis of the C19–C39 fragment of the antimalarial natural product bastimolide A via addition of a functionalized C19–C26 alkyne fragment to a C27–C39 aldehyde fragment. Opening of a terminal epoxide and Noyori asymmetric reduction were used as key steps in the synthesis.


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Large numbers of novel bioactive secondary metabolites have been isolated from marine organisms and many of them are under clinical development against various diseases.[1] Bastimolide A (1, Figure [1]), a polyhydroxylated macrolide, was isolated by Gerwick and co-workers from the cyanobacterium Okeaniahirsuta collected from the Caribbean coast of Panama.[2] Detailed NMR studies, followed by single-crystal X-ray diffraction study of a nona-p-nitrobenzoate derivative confirmed its structure. Bastimolide A has shown antimalarial activity against four resistant strains of P. falciparum with IC50 values between 80 and 270 nM. Its attractive architecture along with potent antimalarial activity and our interest in the total synthesis of complex natural products[3] drew our attention to attempt its total synthesis. So far only one synthetic study has been reported by Quintard et al. where the synthesis of the C15–C27 fragment of bastimolide A was achieved by implementing enantioselective catalytic reactions.[4] In this communication, we report the synthesis of a key C19–C39 fragment of bastimolide A.

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Figure 1 Bastimolide A (1)

Retrosynthetically, bastimolide A could be synthesized (Scheme [1]) from aldehyde 2 that would be obtained from 3 via deprotection of the PMB ether, followed by oxidation of the resulting alcohol. Fragment 3 might be obtained through coupling of alkyne 5 and aldehyde 4.

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Scheme 1 Retrosynthetic analysis

Following this retrosynthetic plan, the synthesis of 5 was commenced (Scheme [2]) from known epoxy alcohol 6, which was prepared from 3-[(4-methoxybenzyl)oxy]propan-1-ol according to the reported procedure.[5] TBS protection of 6 under standard conditions afforded 7 in low yield (30%). However TBS protection in presence of AgNO3 gave 7 in good yield.[6] Epoxide 7 was then opened with lithium trimethylsilylacetylide[6] in the presence of BF3·Et2O to give homopropargylic alcohol 8. Both the silyl protecting groups were removed with TBAF[7] to give diol 9 that, on acetonide protection,[8] furnished the alkyne fragment 5.

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Scheme 2 Synthesis of compound 5

The synthesis of aldehyde fragment 4 is depicted in Scheme [3]. Opening of known epoxide 10 [9] with the anion of trimethylsilylacetylene[10] gave compound 11 that on TMS deprotection followed by TBS protection of 12 gave compound 13 in 91% yield. Addition of alkyne 13 to the known aldehyde 20 [11] gave a diastereomeric mixture of alcohol 14 (1:1) that on DMP[12] oxidation followed by asymmetric reduction of the resulting ketone 15 under Noyori conditions[13] furnished diastereomerically pure compound 16 (dr 97:3). TBS protection of the alcohol 16 followed by one-pot benzyl deprotection and reduction of the alkyne functionality gave primary alcohol 18 that on DMP oxidation furnished the aldehyde fragment 4.

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Scheme 3 Synthesis of compound 4

Having both the fragments 4 and 5 in hand, we planned their coupling (Scheme [4]). Accordingly, alkyne 5 on treatment with n-BuLi followed by reaction of the resulting anion with the aldehyde 4 furnished alcohol 21, oxidation of which with DMP afforded the corresponding ynone. Asymmetric reduction of this under Noyori conditions, followed by TBS protection of the resulting alcohol, afforded the pure C19–C39 fragment 3 of bastimolide A after chromatography.

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Scheme 4 Synthesis of the C19–C39 fragment of bastimolide A

In summary, a convergent approach for a convenient synthesis of C19–C39 fragment of bastimolide A has been achieved via addition of alkyne 5 to an aldehyde 4. The synthesis was completed in 17 steps from known compounds 10 and 6 with an overall yield of 6.9%. Key reactions used in the synthesis include opening of a terminal epoxide with an alkyne anion and asymmetric reduction of the ynone. Furthermore, the chemistry reported here can be used for the large-scale preparation of 3.

All the starting materials, reagents, and solvents were used as received without further purification, unless otherwise stated. Reactions were monitored by thin-layer chromatography (TLC) carried out on silica-coated plates and components were visualized with UV light. 1H NMR and 13C NMR spectra were recorded at 500 and 400 MHz on Bruker spectrometers in either CDCl3 or DMSO-d 6 with TMS as an internal standard. IR spectra were obtained on a Bruker Alpha spectrophotometer (Opus 8.2). Mass spectra were obtained on an AB SCIEX QTrap 5500 LCMS/MS system. The mass spectra (HRMS) were recorded using either a TOF or double focusing spectrometer.


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tert-Butyl({(S)-4-[(4-Methoxybenzyl)oxy]-1-[(S)-oxiran-2-yl]butan­-2- yl}oxy)dimethyl Silane (7)

To a stirred solution of compound 6 (5.0 g, 19.8 mmol) in anhydrous CH2Cl2 (100 mL) were added sequentially TBSCl (5.97 g, 39 mmol), AgNO3 (6.74 g, 39 mmol), and pyridine (1.75 mL, 21 mmol) at 0 °C under nitrogen, and the mixture was stirred for 1 h at 0 °C and 2 h at rt. The reaction mixture was quenched with aq NaHCO3 (60 mL) and extracted with EtOAc (2 × 200 mL). The combined organic layers were washed with H2O (100 mL) and brine (50 mL), dried over Na2SO4, filtered, and concentrated under vacuum. Purification of the crude product by column chromatography (SiO2, 15% EtOAc in hexane) afforded the pure compound 7 (6.31 g, 87%) as a colorless oil; Rf = 0.4 (SiO2, 30% EtOAc in PE); [α]D 26 –15.1 (c 1.8, CHCl3).

IR (neat): νmax = 2933, 2378, 2115, 1513, 1463, 1248, 1037, 832, 678 cm–1.

1H NMR (300 MHz, CDCl3): δ = 7.25 (d, J = 8.6 Hz, 2 H), 6.87 (d, J = 8.6 Hz, 2 H), 4.41 (q, J = 8.4 Hz, 2 H), 4.06 (p, J = 6.0 Hz, 1 H), 3.81 (s, 3 H), 3.52 (t, J = 6.5 Hz, 2 H), 3.04 (m, 1 H), 2.75 (t, J = 4.5 Hz, 1 H), 2.44 (dd, J = 5.0, 2.7 Hz, 1 H), 1.85 (q, J = 6.4 Hz, 2 H), 1.68 (t, J = 5.6 Hz, 2 H), 0.88 (s, 9 H), 0.06 (s, 6 H).

13C NMR (125 MHz, CDCl3): δ = 159.3, 130.8, 129.5, 114.0, 72.8, 67.6, 66.7, 55.5, 49.4, 47.0, 40.6, 37.3, 26.0, 18.2, –4.3, –4.5.

MS (ESI): m/z = 389 [M + Na]+.

HRMS: m/z calcd for C20H34O4SiNa [M + Na]+: 389.2119; found: 389.2124.


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(4R,6S)-6-[(tert-Butyldimethylsilyl)oxy]-8-[(4-methoxybenzyl)oxy]-1-(trimethylsilyl)oct-1-yn-4-ol (8)

To a stirred solution of TMS-acetylene (3 mL, 43.5 mmol) in anhydrous THF (40 mL) was added n-BuLi (23.84 mL, 1.6 M in hexane 38.14 mmol) at –78 °C under argon, and the mixture was stirred for 30 min. BF3·OEt2 (0.7 mL, 50 mmol) was added to the reaction mixture, and it was stirred for 15 min. Compound 7 (dissolved in THF 2 × 10 mL; 4 g, 10.9 mmol) was added via cannula to the above reaction mixture, and the resultant mixture was stirred at –78 °C. After completion of reaction (TLC), aqueous NH4Cl (40 mL) was added, and the reaction mixture allowed to reach rt. The mixture was extracted with EtOAc (2 × 150 mL), the combined organic layers were washed with H2O (100 mL) and brine (50 mL), dried over Na2SO4, filtered, and concentrated under vacuum. Purification of the crude product by column chromatography (SiO2, 20% EtOAc in hexane) afforded pure compound 8 (4.30 g, 85%) as a colorless oil; Rf = 0.6 (SiO2, 30% EtOAc in PE); [α]D 23 +1.2 (c 1.4, CHCl3).

IR (neat): νmax = 3462, 2928, 2174, 1612, 1512, 1249, 1090, 840, 651 cm–1.

1H NMR (400 MHz, CDCl3): δ = 7.24 (d, J = 8.4 Hz, 2 H), 6.88 (d, J = 8.7 Hz, 2 H), 4.42 (d, J = 1.5 Hz, 2 H), 4.08 (m 1 H), 3.87 (m, 1 H), 3.80 (s, 3 H), 3.50 (t, J = 5 Hz, 2 H), 3.00 (br s, 1 H), 2.40 (dd, J = 6.1, 3.3 Hz, 2 H), 1.87–1.78 (m, 3 H), 1.64 (m, 2 H), 0.88 (s, 9 H), 0.15 (s, 9 H), 0.09 (s, 3 H), 0.08 (s, 3 H).

13C NMR (125 MHz, CDCl3): δ = 159.4, 130.6, 129.5, 114.0, 103.6, 87.4, 72.9, 69.7, 68.7, 66.6, 55.46, 42.7, 37.6, 29.2, 26.0, 18.1, 0.3, –4.1, –4.4.

MS (ESI): m/z = 465 [M + H]+.

HRMS: m/z calcd for C25H45O4Si2 [M+H]+: 465.2851; found: 465.2856.


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(3S,5R)-1-[(4-Methoxybenzyl)oxy]oct-7-yne-3,5-diol (9)

To a solution of compound 8 (3.0 g, 6.4 mmol) in anhydrous THF (30 mL) at 0 °C, was added TBAF (32 mL, 32 mmol, 1 M) and the mixture was stirred for 30 min at 0 °C then 30 min at rt. After completion of reaction (TLC), aqueous NH4Cl (20 mL) was added, and the mixture extracted with EtOAc (2 × 100 mL). The combined organic extracts were washed with H2O (20 mL), brine (20 mL), and dried over Na2SO4. After filtration, the solvent was removed under vacuum, and the crude product was purified by column chromatography (SiO2, 25% EtOAc in hexane) to afford pure compound 9 (1.59 g, 89%) as a colorless oil; Rf = 0.6 (SiO2, 40% EtOAc in PE); [α]D 23 +6.5 (c 1.8, CHCl3).

IR (neat): νmax = 3396 (br), 3291 (br), 2922, 2312, 1611, 1512, 1245, 1086, 1032, 821, 644 cm–1.

1H NMR (400 MHz, CDCl3): δ = 7.24 (d, J = 8.5 Hz, 2 H), 6.88 (d, J = 8.5 Hz, 2 H), 4.44 (s, 2 H), 4.09–3.97 (m, 3 H), 3.88 (m, 1 H), 3.79 (s, 3 H), 3.72–3.60 (m, 2 H), 2.44–2.29 (m, 2 H), 2.03 (t, J = 2.7 Hz, 1 H), 1.72 (m, 4 H).

13C NMR (125 MHz, CDCl3): δ = 159.4, 129.9, 129.5, 114.0, 81.1, 73.1, 72.3, 70.7, 70.6, 68.6, 55.4, 42.0, 36.9, 27.5.

MS (ESI): m/z = 301 [M + Na]+.

HRMS: m/z calcd for C16H22O4Na [M + Na]+: 301.1410; found: 301.1419.


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(4S,6R)-4-{2-[(4-Methoxybenzyl)oxy]ethyl}-2,2-dimethyl-6-(prop-2-yn-1-yl)-1,3-dioxane (5)

To a solution of diol 9 (1.0 g, 3.6 mmol) in anhydrous CH2Cl2 (10 mL) at 0 °C was added 2,2-dimethoxypropane (0.65 mL, 5.38 mmol) and CSA (417 mg, 1.79 mmol), and the mixture was stirred for 2 h. After completion of reaction (TLC), aqueous NaHCO3 (5 mL) was added, and the mixture was extracted with EtOAc (3 × 20 mL). The combined organic layers were washed with H2O (10 mL), brine (10 mL), and dried over Na2SO4. After filtration, evaporation of the organic extract under vacuum gave crude product that, on purification by column chromatography (SiO2, 12% EtOAc in hexane), afforded the pure 5 (919 mg, 92%) as a colorless oil; Rf = 0.3 (SiO2, 30% EtOAc in PE); [α]D 26 –26 (c 2.2, CHCl3).

IR (neat): νmax = 3394, 2994, 2377, 2173, 1512, 1245, 1093, 816, 648 cm–1.

1H NMR (400 MHz, CDCl3): δ = 7.25 (d, J = 8.0 Hz, 2 H), 6.88 (d, J = 8.0 Hz, 2 H), 4.45 (d, J = 11.6 Hz, 1 H), 4.42 (d, J = 11.6 Hz, 1 H), 4.07–3.93 (m, 2 H), 3.81 (s, 3 H), 3.59–3.48 (m, 2 H), 2.47 (dd, J = 2.8, 5.2 Hz, 1 H), 2.42 (dd, J = 2.8, 5.2 Hz, 1 H), 2.00 (t, J = 2.7 Hz, 1 H), 1.79–1.71 (m, 2 H), 1.43 (s, 3 H), 1.38 (s, 3 H), 1.28–1.13 (m, 2 H).

13C NMR (100 MHz, CDCl3): δ = 159.3, 130.8, 129.4, 114.0, 99.0, 80.5, 72.8, 70.5, 68.0, 66.2, 66.0, 55.4, 36.7, 36.3, 30.3, 26.4, 20.0.

MS (ESI): m/z = 341 [M + Na]+.

HRMS: m/z calcd for C19H26O4Na [M + Na]+: 341.1723; found: 341.1719.


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(R)-8-(Benzyloxy)-1-(trimethylsilyl)oct-1-yn-4-ol (11)

A stirred solution of TMS-acetylene (8.5 mL, 60.6 mmol) in anhydrous THF (60 mL) was treated with n-BuLi (22.75 mL, 1.6 M in hexane, 36.44 mmol) at –78 °C under nitrogen, and the mixture was stirred for 30 min. BF3 ·OEt2 (1.56 mL, 12.1 mmol) was then added and the mixture was stirred for 30 min. Epoxide 10 (5.0 g, 24.2 mmol) dissolved in anhydrous THF (2 × 10 mL) was added via cannula, and the mixture was stirred for 2 h at –78 °C. A saturated solution of aqueous NH4Cl (80 mL) was added, and the mixture was allowed to reach rt. The reaction mixture was extracted with EtOAc (2 × 250 mL), and the combined organic extracts were washed with brine (50 mL) and dried over anhydrous Na2SO4. Filtration and evaporation of the organic solvents under reduced pressure provided a crude resisdue that, on purification by column chromatography (SiO2, 8% EtOAc/hexane), furnished compound 11 as a colorless oil (6.26 g, 85%). Rf = 0.3 (SiO2, 10% EtOAc/hexane); [α]D 27 –12.1 (c 1.9, CHCl3).

IR (neat): νmax = 3423 (br), 2937,2861, 1495, 1432, 1094, 1027, 843, 747, 697, 632 cm–1.

1H NMR (500 MHz, CDCl3): δ = 7.29–7.25 (m, 4 H), 7.23–7.18 (m, 1 H), 4.43 (s, 2 H), 3.66 (m, 1 H), 3.41 (t, J = 6.5 Hz, 2 H), 2.37 (dd, J = 17, 5.0, Hz, 1 H), 2.27 (dd, J = 17, 2.0, Hz, 1 H), 1.99 (br s, 1 H), 1.63–1.53 (m, 2 H), 1.51–1.42 (m, 3 H), 1.41–1.32 (m, 1 H), 0.09 (s, 9 H).

13C NMR (100 MHz, CDCl3): δ = 138.7, 128.6, 127.8, 127.7, 103.5, 87.8, 73.1, 70.4, 70.0, 36.1, 29.8, 29.0, 22.5, 0.3.

MS (ESI): m/z = 305 [M + H]+.

HRMS: m/z calcd for C18H29O2Si [M + H]+: 305.1931; found: 305.1931.


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(R)-8-(Benzyloxy)oct-1-yn-4-ol (12)

Compound 11 (5.0 g, 16.4 mmol) was dissolved in anhydrous MeOH (150 mL) and treated with anhydrous K2CO3 (4.53 g, 32.8 mmol) at 0 °C under nitrogen. The reaction mixture was stirred at rt for 2 h and quenched with H2O (50 mL). MeOH was removed under reduced pressure, and the aqueous layer was extracted with EtOAc (2 × 80 mL). The combined organic extracts were washed with water (2 × 40 mL) and brine (20 mL). After drying over anhydrous Na2SO4 and filtering, the solvent was evaporated under reduced pressure to give a residue that, on purification by flash chromatography (SiO2, 20% EtOAc/hexane), afforded 12 as a colorless oil (3.20 g, 84%). Rf = 0.6 (SiO2, 20% EtOAc/ hexane); [α]D 30 –5.4 (c 2.6, CHCl3).

IR (neat): νmax = 3418 (br) 3305, 2937, 2862, 2117, 1495, 1432, 1248, 1094, 748, 665, 632 cm–1.

1H NMR (400 MHz, CDCl3): δ = 7.28–7.18 (m, 5 H), 4.43 (s, 2 H), 3.68 (m, 1 H), 3.41 (t, J = 6.4 Hz, 2 H), 2.30 (ddd, J = 16.5, 4.8, 2.4 Hz, 1 H), 2.30 (ddd, J = 16.5, 6.8, 2.8 Hz, 1 H), 1.97 (t, J = 2.8 Hz, 1 H), 1.92 (br s, 1 H), 1.62–1.34 (m, 6 H).

13C NMR (125 MHz, CDCl3): δ = 138.59, 128.45, 127.75, 127.63, 81.10, 72.99, 70.85, 70.27, 69.79, 35.99, 29.63, 27.43, 22.41.

MS (ESI): m/z = 233 [M + H]+.

HRMS: m/z calcd for C15H21O2 [M + H]+: 233.1541; found: 233.1536.


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(R)-{[8-(Benzyloxy)oct-1-yn-4-yl]oxy}(tert-butyl)dimethylsilane (13)

Compound 12 (3.2.0 g, 13.7 mmol) was dissolved in anhydrous CH2Cl2 (60 mL) and treated with 2,6-lutidine (3.68 mL, 34.4 mmol) and TBSOTf­ (4.96 mL, 20 mmol) at 0 °C. The reaction mixture was stirred for 2 h, quenched with saturated aqueous NaHCO3 (20 mL) and extracted with EtOAc (2 × 120 mL). The combined organic extracts were washed with saturated aqueous CuSO4 (2 × 40 mL), water (2 × 40 mL), and brine (50 mL). After drying the organic extract over anhydrous Na2SO4 and filtering, the solvent was evaporated under reduced pressure, and the crude product was purified by column chromatography (SiO2, 20% EtOAc/hexane), which afforded the title compound 13 as a colorless oil (4.32 g, 91%). Rf = 0.3 (SiO2, 20% EtOAc/hexane); [α]D 30 +12.5 (c 1.8, CHCl3).

IR (neat): νmax = 3311, 2930, 2856, 2349, 1389, 1253, 1102, 834, 774, 698 cm–1.

1H NMR (400 MHz, CDCl3): δ = 7.28–7.18 (m, 5 H), 4.43 (s, 2 H), 3.72 (m, 1 H), 3.41 (t, J = 6.6 Hz, 2 H), 2.26–2.21 (m, 2 H), 1.89 (t, J = 2.7 Hz, 1 H), 1.61–1.52 (m, 3 H), 1.51–1.41 (m, 2 H), 1.29 (m, 1 H), 0.81 (s, 9 H), 0.00 (s, 3 H), –0.01 (s, 3 H).

13C NMR (100 MHz, CDCl3): δ = 138.9, 128.5, 127.8, 127.7, 81.9, 73.0, 71.5, 70.5, 70.0, 36.6, 30.0, 27.6, 26.1, 22.1, 18.3, –4.3, –4.4.

MS (ESI): m/z = 347 [M + H]+.

HRMS: m/z calcd for C19H26O4 [M + H]+: 347.2401; found: 347.2401.


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(R)-5-[4-(Benzyloxy)butyl]-2,2,3,3,16,16-hexamethyl-15,15-diphenyl-4,14-dioxa-3,15-disilaheptadec-7-yn-9-one (15)

To a solution of 13 (4.5 g, 13 mmol) in dry THF (70 mL) under argon, n-BuLi (1.6 M in hexane, 9.75 mL, 15.6 mmol) was added at –78 °C. After stirring for 30 min at –78 °C, aldehyde 20 (7.48 g, 16.9 mmol) dissolved in anyhydrous THF (20 mL) was added dropwise via cannula. After stirring for 2.5 h at –78 °C, saturated aqueous NH4Cl (50 mL) was added to the reaction mixture, and the temperature was allowed to rise to rt slowly. The reaction mixture was extracted with EtOAc (2 × 150 mL), and the combined organic extracts were washed with water (2 × 40 mL) and brine (30 mL). After drying the organic extracts with anhydrous Na2SO4 and filtering, the solvent was evaporated under reduced pressure to give a residue that was subjected to flash chromatography (SiO2, 12% EtOAc/hexane) to afford compound 14 as a colorless oil (7.14 g, 80%).

To a solution of compound 14 (7.14 g, 10.3 mmol) in CH2Cl2 (100 mL) at 0 °C was added NaHCO3 (2.2 g, 25.7 mmol) under nitrogen. Then DMP (8.8 g, 20 mmol) was added portionwise at the same temperature. The reaction mixture was allowed to warm to rt and stirred for 4 h. Saturated aqueous Na2S2O3 (50 mL) and saturated aqueous NaHCO3 (30 mL) were added, and the resultant biphasic mixture was stirred for 50 min and then extracted with EtOAc (2 × 150 mL). The combined organic extracts were washed with water (50 mL) and brine (40 mL). The organic extracts wer dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the residue by column chromatography (SiO2, 10% EtOAc/hexane) afforded compound 15 as a colorless oil (6.76 g, 95%). Rf = 0.5 (SiO2, 20% EtOAc/ hexane­); [α]D 30 +3.2 (c 1.5, CHCl3).

IR (neat): νmax = 2931, 2856, 2214, 1716, 1427, 1247, 1110, 849, 701, 606 cm–1.

1H NMR (400 MHz, CDCl3): δ = 7.63–7.57 (m, 4 H), 7.38–7.26 (m, 8 H), 7.25–7.16 (m, 2 H), 4.43 (s, 2 H), 3.78 (pent, J = 6 Hz, 1 H), 3.59 (t, J = 6.2 Hz, 2 H), 3.40 (t, J = 6.4 Hz, 2 H), 2.45 (t, J = 7.2 Hz, 2 H), 2.41 (d, J = 6 Hz, 2 H), 1.69 (pent, J = 7.2 Hz, 2 H), 1.58–1.47 (m, 6 H), 1.44–1.26 (m, 2 H), 0.98 (s, 9 H), 0.82 (s, 9 H), 0.01 (s, 3 H), 0.00 (s, 3 H).

13C NMR (100 MHz, CDCl3): δ = 188.1, 138.8, 135.7, 134.1, 129.8, 128.5, 127.8, 127.8, 127.7, 91.4, 82.4, 73.1, 70.5, 70.4, 63.6, 45.4, 37.0, 31.9, 29.9, 28.0, 27.0, 26.0, 22.0, 20.7, 19.4, 18.21, –4.3, –4.4.

MS (ESI): m/z = 685 [M + H]+.

HRMS: m/z calcd for C42H61O4Si2 [M + H]+: 685.4103; found: 685.4107.


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(5R,9S)-5-[4-(Benzyloxy)butyl]-2,2,3,3,16,16-hexamethyl-15,15-diphenyl-4,14-dioxa-3,15-disilaheptadec-7-yn-9-ol (16)

Alkynone 15 (6.5 g, 9.48 mmol) was dissolved in i-PrOH (120 mL) at rt and treated with Ru[(S,S)-Tsdpen](p-cymene) (30 mg, 948 μmol). After stirring for 36 h at rt, the solvent was removed under reduced pressure to give the crude product, that on purification by column chromatography (SiO2, 20% EtOAc/hexane) afforded compound 16 as a colorless oil (5.4 g, 83%). Rf = 0.3 (SiO2, 20% EtOAc/hexane); [α]D 29 +5.09 (c 16.5, CHCl3).

IR (neat): νmax = 3443 (br), 2930, 2857, 2214, 1414, 1254, 1106, 824, 775, 700, 613 cm–1.

1H NMR (400 MHz, CDCl3): δ = 7.63–7.57 (m, 4 H), 7.36–7.28 (m, 6 H), 7.27 (m, 3 H), 7.23–7.17 (m, 2 H), 4.43 (s, 2 H), 4.24 (m, 1 H), 3.71 (pent, 6.4 Hz), 3.60 (t, J = 6.3 Hz, 2 H), 3.40 (t, J = 6.5 Hz, 2 H), 2.27–2.25 (m, 2 H), 1.72 (d, J = 4.8 Hz, 1 H), 1.60–1.49 (m, 7 H), 1.45 (m, 3 H), 1.34–1.30 (m, 1 H), 0.98 (s, 9 H), 0.81 (s, 9 H), 0.00 (s, 3 H), –0.01 (s, 3 H).

13C NMR (100 MHz, CDCl3): δ = 138.8, 135.8, 134.2, 129.7, 128.5, 127.8, 127.7, 83.1, 82.7, 73.1, 71.1, 70.5, 63.9, 62.9, 3f8.0, 36.8, 32.4, 30.0, 27.8, 27.1, 26.1, 22.1, 21.7, 19.4, 18.3, –4.2, –4.4.

MS (ESI): m/z = 669 [M + NH4]+.

HRMS: m/z calcd for C42H66O4Si2N [M + NH4]+: 704.4525; found: 704.4524.


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(5R,9S)-5-[4-(Benzyloxy)butyl]-2,2,3,3,16,16-hexamethyl-15,15-diphenyl-4,14-dioxa-3,15-disilaheptadec-7-yn-9-ol (17)

Compound 16 (6.0 g, 8.7 mmol) was dissolved in anhydrous CH2Cl2 (100 mL) and treated with 2,6-lutidine (2 mL, 17.4 mmol) and TBSOTf (3.12 mL, 13.1 mmol) at 0 °C. The reaction mixture was stirred for 1 h, quenched with saturated aqueous NaHCO3 (20 mL), and extracted with EtOAc (2 × 150 mL). The combined organic extracts were washed with saturated aqueous CuSO4 (2 × 50 mL), water (50 mL), and brine (50 mL). After drying the organic extract over anhydrous Na2SO4 and filtering, the solvent was evaporated under reduced pressure, and the residue was purified by column chromatography (SiO2, 20% EtOAc/ hexane) to afford 17 as a colorless oil (6.69 g, 96%). Rf = 0.3 (SiO2, 20% EtOAc/hexane); [α]D 30 +5.2 (c 3.3, CHCl3).

IR (neat): νmax = 2937, 2858, 2349, 1463, 1370, 1254, 1102, 833, 772, 701, 613 cm–1.

1H NMR (500 MHz, CDCl3): δ = 7.67 (m, 4 H), 7.42–7.33 (m, 8 H), 7.29–7.24 (m, 2 H), 4.50 (s, 2 H), 4.30 (m, 1 H), 3.74 (m, 1 H), 3.65 (t, J = 6.2 Hz, 2 H), 3.46 (t, J = 6.6 Hz, 2 H), 2.34 (ddd, J = 16.5, 5.0, 2.0 Hz 1 H), 2.28 (ddd, J = 16.5, 7.0, 2.0 Hz 1 H) 1.67–1.57 (m, 7 H), 1.53–1.45 (m, 4 H), 1.38–1.33 (m, 1 H), 1.04 (s, 9 H), 0.90 (s, 9 H), 0.88 (s, 9 H), 0.11 (s, 3 H), 0.09 (s, 3 H), 0.06 (s, 3 H), 0.04 (s, 3 H).

13C NMR (125 MHz, CDCl3): δ = 138.9, 135.8, 134.3, 129.7, 128.5, 127.8, 127.7, 83.8, 81.5, 73.1, 71.3, 70.6, 64.1, 63.4, 39.0, 36.6, 32.5, 30.1, 28.0, 27.1, 26.1, 26.1, 22.1, 22.0, 19.4, 18.5, 18.3, –4.2, –4.5, –4.7.

MS (ESI): m/z = 818 [M + NH4]+.

HRMS: m/z calcd for C48H80O4Si3N [M + NH4]+: 818.5390; found: 818.5403.


#

(5R,9S)-5-[(tert-Butyldimethylsilyl)oxy]-13-[(tert-butyldiphenylsilyl)oxy]tridecane-1,9-diol (18)

A stirred solution of compound 17 (6.5 g, 8.1 mmol) in anhydrous EtOAc (100 mL) was treated with 10% Pd/C (86 mg, 20% w/w) and hydrogenated using a hydrogen-filled balloon at rt for 5 h. The reaction mixture was filtered through a Celite plug, and the plug was washed with EtOAc (2 ×100 mL). The combined filtrate and washings were concentrated under reduced pressure to give the crude product, which was purified by silica gel column chromatography (SiO2, 100–200 mesh, 10% EtOAc/hexane) to afford compound 18 (4.92 g 85%) as a colorless oil. Rf = 0.5 (SiO2, 20% EtOAc/hexane); [α]D 25 –2.3 (c 1.8, CHCl­3).

IR (neat): νmax = 3071 (br), 2929, 2856, 1413, 1390, 1253, 1108, 833, 772, 700, 613 cm–1.

1H NMR (500 MHz, CDCl3): δ = 7.68–7.66 (m, 4 H), 7.44–7.34 (m, 6 H), 3.66–3.60 (m, 6 H), 1.59–1.53 (m, 6 H), 1.45–1.36 (m, 12 H), 1.04 (s, 9 H), 0.88 (s, 9 H), 0.87 (s, 9 H), 0.03 (s, 6 H), 0.03 (s, 3 H), 0.02 (s, 3 H).

13C NMR (100 MHz, CDCl3): δ = 135.8, 134.3, 129.7, 127.8, 72.5, 72.4, 64.2, 63.2, 37.6, 37.1, 36.9, 33.2, 33.1, 30.0, 29.8, 27.1, 26.2, 21.9, 21.6, 21.2, 19.4, 18.4, –4.2.

MS (ESI): m/z = 716 [M + H]+.

HRMS: m/z calcd for C41H78O4Si3N [M + NH4]+: 732.5232; found: 732.5233.


#

(5S,9S,13S)-9,13-bis[(tert-Butyldimethylsilyl)oxy]-5-{3-[(4R,6S)-6-{2-[(4-methoxybenzyl)oxy]ethyl}-2,2-dimethyl-1,3-dioxan-4-yl]prop-1-yn-1-yl}-2,2,3,3,20,20-hexamethyl-19,19-diphenyl-4,18-dioxa-3,19-disilahenicosane (3)

Alcohol 18 (200 mg, 0.28 mmol) was dissolved in anhydrous CH2Cl2 (10 mL) and the solution cooled to 0 °C. NaHCO3 (84 mg, 0.97 mmol) and DMP (294 mg, 0.68 mmol) were added sequentially at the same temperature, and the resulting reaction mixture was stirred under nitrogen for 3 h at rt. Saturated aqueous Na2S2O3 (10 mL) and saturated aqueous NaHCO3 (10 mL) were added to the reaction mixture, and the resultant biphasic mixture was stirred for 30 min. The mixture was extracted with EtOAc (2 × 15 mL), and the combined organic extracts were washed with water (10 mL) and brine (10 mL), dried over anhydrous Na2SO4, and filtered. The solvents were evaporated under reduced pressure to give aldehyde 4 (175 mg, 88%; Rf = 0.4, 10% EtOAc/ hexane), which was used directly for the next reaction.

To a stirred solution of 5 (238 mg, 0.75 mmol) in dry THF (8 mL) under nitrogen, n-BuLi (1.6 M in hexane, 0.4 mL, 0.625 mmol) was added at –78 °C. After stirring for 30 min at –78 °C, crude aldehyde 4 (175 mg, 0.25 mmol) dissolved in THF (2 × 4 mL) was added dropwise via cannula. After stirring for 45 min at –78 °C , saturated aqueous NH4Cl (5 mL) was added, and the temperature was allowed to raise to rt slowly. The reaction mixture was extracted with EtOAc (2 × 15 mL) and the combined organic extracts washed with water (10 mL) and brine (10 mL). After drying the organic extracts over anhydrous Na2SO­4 and filtering, the solvent was evaporated under reduced pressure to give a crude product that was subjected to flash chromatography (SiO2, 15% EtOAc/hexane) to afford 21 as a mixture of diastereomers as a colorless oil (218 mg, 85%).

To a solution of compound 21 (218 mg, 0.21 mmol) in CH2Cl2 (10 mL) at 0 °C was added NaHCO3 (54 mg, 0.63 mmol) under nitrogen, then DMP (178 mg, 0.42 mmol) was added portion-wise. The reaction mixture was warmed to rt and stirred for 4 h. Saturated aqueous Na2S2O3 (5 mL) and saturated aqueous NaHCO3 (5 mL) were added, and the resultant biphasic mixture was stirred for 10 min and then extracted with EtOAc (2 × 15 mL). The combined organic extracts were washed with water (10 mL) and brine (10 mL), dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the residue by column chromatography (SiO2, 10% EtOAc/ hexane) afforded the ketone as a colorless oil (164 mg, 76%). Rf = 0.4 (SiO2, 20% EtOAc/hexane).

The purified alkynone (164 mg, 0.16 mmol) was dissolved in i-PrOH (4 mL) at rt and treated with Ru[(S,S)-Tsdpen](p-cymene) (5 mg, 8 μmol). After stirring for 48 h at rt, the solvent was removed under reduced pressure to give that crude product that, on purification by flash chromatography (SiO2, 15% EtOAc/hexane), afforded the alcohol compound as a colorless oil (143 mg, 87%). Rf = 0.6 (SiO2, 20% EtOAc/ hexane).

The purified alcohol (143 mg, 0.14 mmol) was dissolved in anhydrous CH2Cl2 (5 mL) and treated with 2,6-lutidine (16 μmL, 0.27 mmol) and TBSOTf (50 μmL, 0.21 mmol) at 0 °C. The reaction mixture was stirred for 1 h and quenched with saturated aqueous NaHCO3 (5 mL) and extracted with EtOAc (2 × 10 mL). The combined organic extracts were washed with saturated aqueous CuSO4 (2 × 8 mL), water (2 × 8 mL), and brine (10 mL). After drying over anhydrous Na2SO4 and filtering, the solvent was evaporated under reduced pressure, and the residue was purified by column chromatography (SiO2, 4% EtOAc/hexane), which afforded the target 3 as a colorless oil (111 mg, 69%). Rf = 0.4 (SiO2, 20% EtOAc/hexane); [α]D 30 –15.9 (c 1.8, CHCl3).

IR (neat): νmax = 2929, 2856, 1514, 1464, 1249, 1106, 835, 773, 702 cm–1.

1H NMR (400 MHz, CDCl3): δ = 7.70–7.64 (m, 4 H), 7.44 (m, 6 H), 7.25–7.22 (m, 2 H), 6.91–6.85 (m, 2 H), 4.43 (q, J = 11.7 Hz, 2 H), 4.31 (m, 1 H), 4.07–3.88 (m, 2 H), 3.80 (s, 3 H), 3.68–3.47 (m, 6 H), 2.35 (dd, J = 16.5, 4.8 Hz, 1 H), 2.59 (dd, J = 16.5, 3.6 Hz, 1 H),1.88–1.66 (m, 3 H), 1.66–1.58 (m, 4 H), 1.58–1.43 (m, 5 H), 1.42 (s, 3 H), 1.41–1.37 (m, 7 H), 1.41 (s, 3 H), 1.37 (s, 3 H), 1.05 (s, 9 H), 0.89 (s, 9 H), 0.88 (s, 18 H), 0.11 (s, 3 H), 0.09 (s, 3 H), 0.03 (s, 12 H).

13C NMR (100 MHz, CDCl3): δ = 159.4, 135.8, 134.4, 131.7, 130.8, 130.8, 129.7, 129.4, 127.8, 114.0, 99.0, 84.2, 80.2, 72.9, 72.5, 72.5, 68.2, 66.2, 66.1, 64.2, 63.4, 55.5, 39.5, 37.7, 37.7, 37.1, 37.0, 36.8, 36.6, 33.1, 30.3, 27.1, 26.8, 26.2, 26.1, 21.9, 21.5, 21.3, 20.1, 19.4, 18.5, 18.4, –4.1, –4.7.

MS (ESI): m/z = 1162 [M + NH4]+.

HRMS: m/z calcd for C66H116O8Si4N [M + NH4]+: 1162.7773; found: 1162.7772.


#
#

Conflict of Interest

The authors declare no conflict of interest.

Acknowledgment

We wish to thank the KIM department for providing the IICT ccommunication No. IICT/Pubs./2021/254.

Supporting Information

  • References

    • 1a Pallela R, Na-Young Y, Kim SK. Mar. Drugs 2010; 8: 1189
    • 1b Kuttruff CA, Eastgate MD, Baron PS. Nat. Prod. Rep. 2014; 31: 419
    • 1c Blunt JW, Copp BR, Keyzers RA, Munro MH. G, Prinsep MR. Nat. Prod. Rep. 2012; 29: 144
  • 2 Shao C.-L, Linington RG, Balunas MJ, Centeno A, Boudreau P, Zhang C, Engene N, Spadafora C, Mutka TS, Kyle DE, Gerwick L, Wang C.-Y, Gerwick WH. J. Org. Chem. 2015; 80: 7849
    • 3a Ghosh S, Kumar SU, Shashidhar J. J. Org. Chem. 2008; 73: 1582
    • 3b Ghosh S, Pradhan TK. J. Org. Chem. 2010; 75: 2107
    • 3c Athe S, Chandrasekhar B, Roy S, Pradhan TK, Ghosh S. J. Org. Chem. 2012; 77: 9840
    • 3d Reddy KM, Yamini V, Singarapu KK, Ghosh S. Org. Lett. 2014; 16: 2658
    • 3e Chandrasekhar B, Athe S, Reddy PP, Ghosh S. Org. Biomol. Chem. 2015; 13: 115
    • 3f Rao KN, Kanakaraju M, Kunwar AC, Ghosh S. Org. Lett. 2016; 18: 4092
    • 3g Athe A, Sharma A, Marumudi K, Ghosh S. Org. Biomol. Chem. 2016; 14: 6769
    • 3h Sharma A, Athe S, Ghosh S. ACS Omega 2018; 3: 16563
  • 4 Quintard A, Sperandio C, Rodriguez J. Org. Lett. 2018; 20: 5274
  • 5 Schleicher DK, Jamison TF. Beilstein J. Org. Chem. 2013; 9: 1533
  • 7 Sayini R, Srihari P. Synthesis 2018; 50: 663
  • 8 Nicolaou KC, Daines RA, Uenishi J, Li WS, Papahatjis PD, Chakraborty TK. J. Am. Chem. Soc. 1988; 110: 4672
  • 9 Bujaranipalli S, Das S. Tetrahedron: Asymmetry 2016; 27: 254
  • 10 Cook C, Guinchard X, Liron F, Roulland E. Org. Lett. 2010; 12: 744
  • 11 Nilewski C, Deprez NR, Fessard TC, Li D, Geisser RW, Carreira EM. Angew. Chem. Int. Ed. 2011; 50: 7940
  • 12 Dess DB, Martin JC. J. Am. Chem. Soc. 1991; 113: 7277
  • 13 Matsumura K, Hashiguchi S, Ikariya T, Noyori R. J. Am. Chem. Soc. 1997; 119: 8738

Corresponding Author

Subhash Ghosh
Department of Organic Synthesis & Process Chemistry, CSIR-Indian Institute of Chemical Technology
Hyderabad-500007
India   

Publication History

Received: 30 August 2021

Accepted after revision: 16 September 2021

Accepted Manuscript online:
17 September 2021

Article published online:
06 October 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • References

    • 1a Pallela R, Na-Young Y, Kim SK. Mar. Drugs 2010; 8: 1189
    • 1b Kuttruff CA, Eastgate MD, Baron PS. Nat. Prod. Rep. 2014; 31: 419
    • 1c Blunt JW, Copp BR, Keyzers RA, Munro MH. G, Prinsep MR. Nat. Prod. Rep. 2012; 29: 144
  • 2 Shao C.-L, Linington RG, Balunas MJ, Centeno A, Boudreau P, Zhang C, Engene N, Spadafora C, Mutka TS, Kyle DE, Gerwick L, Wang C.-Y, Gerwick WH. J. Org. Chem. 2015; 80: 7849
    • 3a Ghosh S, Kumar SU, Shashidhar J. J. Org. Chem. 2008; 73: 1582
    • 3b Ghosh S, Pradhan TK. J. Org. Chem. 2010; 75: 2107
    • 3c Athe S, Chandrasekhar B, Roy S, Pradhan TK, Ghosh S. J. Org. Chem. 2012; 77: 9840
    • 3d Reddy KM, Yamini V, Singarapu KK, Ghosh S. Org. Lett. 2014; 16: 2658
    • 3e Chandrasekhar B, Athe S, Reddy PP, Ghosh S. Org. Biomol. Chem. 2015; 13: 115
    • 3f Rao KN, Kanakaraju M, Kunwar AC, Ghosh S. Org. Lett. 2016; 18: 4092
    • 3g Athe A, Sharma A, Marumudi K, Ghosh S. Org. Biomol. Chem. 2016; 14: 6769
    • 3h Sharma A, Athe S, Ghosh S. ACS Omega 2018; 3: 16563
  • 4 Quintard A, Sperandio C, Rodriguez J. Org. Lett. 2018; 20: 5274
  • 5 Schleicher DK, Jamison TF. Beilstein J. Org. Chem. 2013; 9: 1533
  • 7 Sayini R, Srihari P. Synthesis 2018; 50: 663
  • 8 Nicolaou KC, Daines RA, Uenishi J, Li WS, Papahatjis PD, Chakraborty TK. J. Am. Chem. Soc. 1988; 110: 4672
  • 9 Bujaranipalli S, Das S. Tetrahedron: Asymmetry 2016; 27: 254
  • 10 Cook C, Guinchard X, Liron F, Roulland E. Org. Lett. 2010; 12: 744
  • 11 Nilewski C, Deprez NR, Fessard TC, Li D, Geisser RW, Carreira EM. Angew. Chem. Int. Ed. 2011; 50: 7940
  • 12 Dess DB, Martin JC. J. Am. Chem. Soc. 1991; 113: 7277
  • 13 Matsumura K, Hashiguchi S, Ikariya T, Noyori R. J. Am. Chem. Soc. 1997; 119: 8738

Zoom Image
Figure 1 Bastimolide A (1)
Zoom Image
Scheme 1 Retrosynthetic analysis
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Scheme 2 Synthesis of compound 5
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Scheme 3 Synthesis of compound 4
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Scheme 4 Synthesis of the C19–C39 fragment of bastimolide A