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Z Geburtshilfe Neonatol 2021; 225(03): 226-231
DOI: 10.1055/a-1206-1064
Original Article

Calcitriol Effect on Outcomes of in Vitro Fertilization in Infertile Women with Vitamin D Deficiency: A Double-Blind Randomized Clinical Trial

Leila Doryanizadeh
1   Department of Midwifery, School of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran (the Islamic Republic of)
,
Bahar Morshed-Behbahani
1   Department of Midwifery, School of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran (the Islamic Republic of)
,
Mohammad Ebrahim Parsanezhad
2   Department of Gynecology and Obstetrics, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran (the Islamic Republic of)
,
Mohammad Hossein Dabbaghmanesh
3   Shiraz endocrine research center, Shiraz University of Medical Sciences, Shiraz, Iran (the Islamic Republic of)
,
Azam Jokar
1   Department of Midwifery, School of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran (the Islamic Republic of)
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Abstract

Introduction Recent studies have addressed the role of micronutrients in fertilization, such as vitamin D. The present study aims to explore the effects of using calcitriol, an active form of vitamin D, on IVF results in women with vitamin D deficiency.

Method This double-blinded randomized clinical trial was done on 180 infertile women, undergone IVF treatment. Out of them, 95 were found to have vitamin D deficiency (blood serum 25-dihydroxy vitamin D <30 ng/ml). Fifty one women in experimental group were treated with two 0.25 µg calcitriol pills daily during 4 weeks (discontinued 8 hours prior to the embryo transfer) and 44 subjects to the placebo group (mean vitamin D deficiency 27.5 ±1.8 in case group vs. 27.6±1.8 in control group, P>0.05). Final analysis includes outcomes of chemical and clinical pregnancy was done on 74 women (including 36 in case and 38 in control group).

Results Our study showed that in the experimental group, chemical pregnancy success was significantly higher than that in the control group, 31.4 vs. 18.2% (P<0.05). However, there were no significant differences between the 2 groups in reaching the clinical pregnancy stage (25.5% in case group vs. 13.6% in control group) and continuation of pregnancy into week 20 (9.8% in case group vs. 11.6% in control group) (P>0.05).

Conclusion Calcitriol administration by improving the implantation process can significantly increase the chances of successful IVF cycle results in infertile women with vitamin D deficiency.

Key words

calcitriol-IVF - infertility - pregnancy rate


Publication History

Received: 30 November 2019

Accepted after revision: 30 May 2020

Article published online:
14 September 2020

© 2020. Thieme. All rights reserved.

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  • References

  • 1 Morshed-Behbahani B, Mossalanejad L, Shahsavari S. et al. The experiences of infertile women on assistant reproductive treatments: a phenomenological study. Iran Red Crescent Med J 2012; 14: 382
    PubMedSearch in Google Scholar
  • 2 Mosalanejad L, Koolaeen AK, Behbahani BM. Looking out for the secret wound: the effect of E-cognitive group therapy with emotional disclosure on the status of mental health in infertile women. Int J Fertil Steril 2012; 6: 87
    PubMedSearch in Google Scholar
  • 3 Barbieri RL. Female infertility. In: Strauss JF, Barbieri RL, Eds. Yen and Jaffeʼs Reproductive Endocrinology. 8th ed. Philadelphia: Elsevier; 2019: 556–581.e7
  • 4 Rudick BJ, Ingles SA, Chung K. et al. Influence of vitamin D levels on in vitro fertilization outcomes in donor-recipient cycles. Fertil Steril 2014; 101: 447-452
    CrossrefPubMedSearch in Google Scholar
  • 5 Soares JB, Beck RT, Neto TBB. et al. Vitamin D: an effect on fertility and in vitro fertilization. Hum Reprod Arch 2018; 32: 1-7
    CrossrefPubMedSearch in Google Scholar
  • 6 Lv SS, Wang JY, Wang XQ. et al. Serum vitamin D status and in vitro fertilization outcomes: a systematic review and meta-analysis. Arch Gynecol Obstet 2016; 293: 1339-1345
    CrossrefPubMedSearch in Google Scholar
  • 7 Nasri H, Behradmanesh S, Maghsoudi AR. et al. Efficacy of supplementary vitamin D on improvement of glycemic parameters in patients with type 2 diabetes mellitus; a randomized double blind clinical trial. J Renal Inj Prev 2014; 3: 31
    PubMedSearch in Google Scholar
  • 8 Skowrońska P, Pastuszek E, Kuczyński W. et al. The role of vitamin D in reproductive dysfunction in women – a systematic review. Ann Agric Environ Med 2016; 23: 671-676
    CrossrefPubMedSearch in Google Scholar
  • 9 Tavakoli M, Jeddi-Tehrani M, Salek-Moghaddam A. et al. Effects of 1, 25 (OH) 2 vitamin D3 on cytokine production by endometrial cells of women with recurrent spontaneous abortion. Fertil Steril 2011; 96: 751-757
    CrossrefPubMedSearch in Google Scholar
  • 10 Maha Q, Laiba M, Rehana R. Vitamin D receptor polymorphism and male factor infertility. J Pak Med Assoc 2019; 69 (4): 603-604
    PubMedSearch in Google Scholar
  • 11 Rudick B, Ingles S, Chung K. et al. Characterizing the influence of vitamin D levels on IVF outcomes. Hum Reprod 2012; 27: 3321-3327
    CrossrefPubMedSearch in Google Scholar
  • 12 Barrera D, Avila E, Hernández G. et al. Calcitriol affects hCG gene transcription in cultured human syncytiotrophoblasts. Reprod Biol Endocrinol 2008; 6: 3
    CrossrefPubMedSearch in Google Scholar
  • 13 Jindal S, Sharma JC, Sharma M. Association of deficiency of maternal vitamin D levels with severity of preeclampsia. Epidemiology International (E-ISSN: 2455-7048) 2019; 4: 10-16
    CrossrefPubMedSearch in Google Scholar
  • 14 Hanif QA, Qamar S, Aslam P. et al. Association of vitamin D deficiency with polycystic ovarian syndrome. Pakistan Armed Forces Medical Journal 2019; 69: 241-44
    PubMedSearch in Google Scholar
  • 15 Kalaitzopoulos DR, Lempesis IG, Athanasakiet F. et al. Association between vitamin D and endometriosis: a systematic review. Hormones 2019; 1-13
    PubMedSearch in Google Scholar
  • 16 Sharef AAR, Hussien SS, Noori FM. Vitamin D3 deficiency and early pregnancy loss. Middle East J Fam Med 2020; 7: 76
    CrossrefPubMedSearch in Google Scholar
  • 17 Aleyasin A, Hosseini MA, Mahdavi A. et al. Predictive value of the level of vitamin D in follicular fluid on the outcome of assisted reproductive technology. Eur J Obstet Gynecol Reprod Biol 2011; 159: 132-137
    CrossrefPubMedSearch in Google Scholar
  • 18 Saki F, Dabbaghmanesh MH, Omrani GR. et al. Vitamin D deficiency and its associated risk factors in children and adolescents in southern Iran. Public Health Nutr 2017; 20: 1851-1856
    CrossrefPubMedSearch in Google Scholar
  • 19 Tabrizi R, Moosazadeh M, Akbari M. et al. High prevalence of vitamin D deficiency among Iranian population: a systematic review and meta-analysis. Iran J Med Sci 2018; 43: 125
    PubMedSearch in Google Scholar
  • 20 Ozkan S, Jindal S, Greenseid K. et al. Replete vitamin D stores predict reproductive success following in vitro fertilization. Fertil Steril 2010; 94: 1314-1319
    CrossrefPubMedSearch in Google Scholar
  • 21 Liu X, Zhang W, Xu Y. et al. Effect of vitamin D status on normal fertilization rate following in vitro fertilization. Reprod Biol Endocrinol 2019; 17: 59
    CrossrefPubMedSearch in Google Scholar
  • 22 Garbedian K, Boggild M, Moody J. et al. Effect of vitamin D status on clinical pregnancy rates following in vitro fertilization. CMAJ Open 2013; 1: E77-82
    CrossrefPubMedSearch in Google Scholar
  • 23 Abedi S, Taebi M, Esfahani MHN. Effect of vitamin D supplementation on intracytoplasmic sperm injection outcomes: a randomized double-blind placebo-controlled trial. Int J Fertil Steril 2019; 13: 18
    PubMedSearch in Google Scholar
  • 24 Kamar SS. Histological evaluation of vitamin-D deficiency on the endometrial decidualization in early pregnancy in rat model. Egypt J Histol 2018; 41: 300-310
    PubMedSearch in Google Scholar
  • 25 Fabris A, Pacheco A, Cruz M. et al. Impact of circulating levels of total and bioavailable serum vitamin D on pregnancy rate in egg donation recipients. Fertil Steril 2014; 102: 1608-1612
    CrossrefPubMedSearch in Google Scholar
  • 26 Lexicomp. Drug Information Handbook with International Trade Names Index. Lexi-Comp; 2016
  • 27 Abdullah UH, Lalani S, Syed F. et al. Association of vitamin D with outcome after intra cytoplasmic sperm injection. J Matern Fetal Neonatal Med 2017; 30: 117-120
    CrossrefPubMedSearch in Google Scholar
  • 28 Aflatoonian A, Arabjahvani F, Eftekhar M. et al. Effect of vitamin D insufficiency treatment on fertility outcomes in frozen-thawed embryo transfer cycles: a randomized clinical trial. Iran J Reprod Med 2014; 12: 595
    PubMedSearch in Google Scholar
  • 29 Franasiak JM, Molinaro TA, Dubell EK. et al. Vitamin D levels do not affect IVF outcomes following the transfer of euploid blastocysts. Am J Obstet Gynecol 2015; 212 (315) e1-e6
    CrossrefPubMedSearch in Google Scholar
  • 30 Paffoni A, Ferrari S, Viganò P. et al. Vitamin D deficiency and infertility: insights from in vitro fertilization cycles. J Clin Endocrinol Metab 2014; 99: E2372-E2376
    CrossrefPubMedSearch in Google Scholar
  • 31 Banker M, Sorathiya D, Shah S. Vitamin D deficiency does not influence reproductive outcomes of IVF-ICSI: A study of oocyte donors and recipients. J Hum Reprod Sci 2017; 10: 79-85
    CrossrefPubMedSearch in Google Scholar