Horm Metab Res 2019; 51(11): 741-748
DOI: 10.1055/a-0926-3991
Endocrine Research
© Georg Thieme Verlag KG Stuttgart · New York

Secreted-Osteopontin Contributes to Brown Adipogenesis In Vitro via a CD44-Dependent Pathway

Mengxi Wang
1   Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang, Jiangxi, P. R. China
2   Department of Pharmacology, School of Pharmacy, Nanchang University, Nanchang, Jiangxi, P. R. China
,
Yaoyao Guo
3   Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, P. R. China
4   Nanchang Joint Programme, Queen Mary University of London, Nanchang, Jiangxi, P. R. China
,
Yumeng Zhou
1   Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang, Jiangxi, P. R. China
2   Department of Pharmacology, School of Pharmacy, Nanchang University, Nanchang, Jiangxi, P. R. China
,
Wanwan Yuan
1   Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang, Jiangxi, P. R. China
2   Department of Pharmacology, School of Pharmacy, Nanchang University, Nanchang, Jiangxi, P. R. China
,
Huixia Li
3   Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, P. R. China
4   Nanchang Joint Programme, Queen Mary University of London, Nanchang, Jiangxi, P. R. China
,
Shaofeng Xiong
1   Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang, Jiangxi, P. R. China
2   Department of Pharmacology, School of Pharmacy, Nanchang University, Nanchang, Jiangxi, P. R. China
,
Kun Wang
1   Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang, Jiangxi, P. R. China
2   Department of Pharmacology, School of Pharmacy, Nanchang University, Nanchang, Jiangxi, P. R. China
,
Guohua Zeng
1   Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang, Jiangxi, P. R. China
2   Department of Pharmacology, School of Pharmacy, Nanchang University, Nanchang, Jiangxi, P. R. China
,
Qiren Huang
1   Key Provincial Laboratory of Basic Pharmacology, Nanchang University, Nanchang, Jiangxi, P. R. China
2   Department of Pharmacology, School of Pharmacy, Nanchang University, Nanchang, Jiangxi, P. R. China
› Author Affiliations
Further Information

Publication History

received 16 November 2018

accepted 14 May 2019

Publication Date:
11 July 2019 (online)

Abstract

Osteopontin (OPN), a secreted glycoprotein, is involved in various pathophysiological processes including immune response, inflammation, tumor formation, and metabolism. OPN exists in 2 forms, secreted-OPN (sOPN) and intracellular-OPN (iOPN). While they might have different biological activities, it remains largely unknown whether sOPN and iOPN induce the differentiation of brown adipocytes. To test this possibility, 3T3-L1 cells were induced by DMI induction with or without recombinant human OPN (rhOPN, 10, 50, 100, 200 μM), respectively. Meanwhile, another batch of 3T3-L1 cells were infected with Ad-GFP-ap2-OPN and followed by DMI differentiation. Subsequently, the infected cells were treated with either anti-CD44 antibody or immunoglobulin G (Ig G). Accumulation of lipid droplets was visualized by Oil red O staining and protein levels were assayed by western blotting analysis. The results showed that sOPN and not rhOPN, notably increased the accumulation of lipid droplets and the expression of brown adipocyte-related genes. Moreover, neutralization of CD44 partially abrogated the effects induced by sOPN. These data demonstrate that sOPN and not rhOPN has the capacity to induce the differentiation of white preadipocytes into brown adipocytes through a CD44-dependent mechanism. The findings might provide a potential target for sOPN to combat obesity.

Supplementary Material

 
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