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DOI: 10.5999/aps.2018.00948
Wound healing effects of paste type acellular dermal matrix subcutaneous injection
This work was supported by a 2017 Yeungnam University Research Grant.
Background Acellular dermal matrix (ADM) helps wound healing by stimulating angiogenesis, acting as a chemoattractant for endothelial cells, providing growth factors, and permitting a substrate for fibroblasts to attach. The current standard for using paste-type ADM (CG Paste) in wound healing is direct application over the wounds. The major concerns regarding this method are unpredictable separation from the wounds and absorption into negative-pressure wound therapy devices. This study aimed to investigate the effects of subcutaneous injection of paste-type ADM on wound healing in rats.
Methods Full-thickness skin defects were created on the dorsal skin of rats. Eighteen rats were randomly divided into three groups and treated using different wound coverage methods: group A, with a saline dressing; group B, standard application of CG Paste; and group C, injection of CG Paste. On postoperative days 3, 5, 7, 10, and 14, the wound areas were analyzed morphologically. Histological and immunohistochemical tissue analyses were performed on postoperative days 3 and 7.
Results Groups B and C had significantly less raw surface than group A on postoperative days 10 and 14. Collagen fiber deposition and microvessel density were significantly higher in group C than in groups A and B on postoperative days 3 and 7.
Conclusions This study showed comparable effectiveness between subcutaneous injection and the conventional dressing method of paste-type ADM. Moreover, the injection of CG Paste led to improved wound healing quality through the accumulation of collagen fibers and an increase in microvessel density.
This article was presented at EWMA 2018 on May 9-11, 2018 in Krakow, Poland.
Publication History
Received: 18 August 2018
Accepted: 23 October 2018
Article published online:
03 April 2022
© 2018. The Korean Society of Plastic and Reconstructive Surgeons. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonCommercial License, permitting unrestricted noncommercial use, distribution, and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes. (https://creativecommons.org/licenses/by-nc/4.0/)
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REFERENCES
- 1 Aramwit P. Introduction to biomaterials for wound healing. Agren MS. Wound healing biomaterials. Cambridge: Woodhead Publishing; 2016: 3-38
- 2 Rakhorst G, Ploeg RJ. Biomaterials in modern medicine: the Groningen perspective. Hackensack: World Scientific Publishing Company; 2008
- 3 Baier RE. Advanced biomaterials development from “natural products”. J Biomater Appl 1988; 2: 615-26
- 4 Brown-Etris M, Cutshall WD, Hiles MC. A new biomaterial derived from small intestine submucosa and developed into a wound matrix device. Wounds 2002; 14: 150-66
- 5 Pizzo AM, Kokini K, Vaughn LC. et al. Extracellular matrix (ECM) microstructural composition regulates local cell-ECM biomechanics and fundamental fibroblast behavior: a multidimensional perspective. J Appl Physiol (1985) 2005; 98: 1909-21
- 6 Hodde JP, Record RD, Liang HA. et al. Vascular endothelial growth factor in porcine-derived extracellular matrix. Endothelium 2001; 8: 11-24
- 7 Li R, Guo W, Yang B. et al. Human treated dentin matrix as a natural scaffold for complete human dentin tissue regeneration. Biomaterials 2011; 32: 4525-38
- 8 Hodde JP, Ernst DM, Hiles MC. An investigation of the long-term bioactivity of endogenous growth factor in OASIS Wound Matrix. J Wound Care 2005; 14: 23-5
- 9 Brigido SA, Schwartz E, McCarroll R. et al. Use of an acellular flowable dermal replacement scaffold on lower extremity sinus tract wounds: a retrospective series. Foot Ankle Spec 2009; 2: 67-72
- 10 Knox RL, Hunt AR, Collins JC. et al. Platelet-rich plasma combined with skin substitute for chronic wound healing: a case report. J Extra Corpor Technol 2006; 38: 260-4
- 11 Banta MN, Eaglstein WH, Kirsner RS. Healing of refractory sinus tracts by dermal matrix injection with Cymetra. Dermatol Surg 2003; 29: 863-6
- 12 Weidner N. Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors. Breast Cancer Res Treat 1995; 36: 169-80
- 13 Boateng JS, Matthews KH, Stevens HN. et al. Wound healing dressings and drug delivery systems: a review. J Pharm Sci 2008; 97: 2892-923
- 14 Barber FA, Aziz-Jacobo J. Biomechanical testing of commercially available soft-tissue augmentation materials. Arthroscopy 2009; 25: 1233-9
- 15 Chen WF, Barounis D, Kalimuthu R. A novel cost-saving approach to the use of acellular dermal matrix (AlloDerm) in postmastectomy breast and nipple reconstructions. Plast Reconstr Surg 2010; 125: 479-81
- 16 Mao J, Yin Y. et al. Chitosan/gelatin network based biomaterials in tissue engineering. Biomed Eng (Singapore) 2002; 14: 115-21
- 17 Crovetti G, Martinelli G, Issi M. et al. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci 2004; 30: 145-51
- 18 Gibson T. Evolution of catgut ligatures: the endeavours and success of Joseph Lister and William Macewen. Br J Surg 1990; 77: 824-5
- 19 Chattopadhyay S, Raines RT. Review collagen-based biomaterials for wound healing. Biopolymers 2014; 101: 821-33