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DOI: 10.5999/aps.2014.41.3.241
Effects of the Diabetic Condition on Grafted Fat Survival: An Experimental Study Using Streptozotocin-Induced Diabetic Rats
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Background Autologous fat grafts have been widely used for cosmetic purposes and for soft tissue contour reconstruction. Because diabetes mellitus is one of the major chronic diseases in nearly every country, the requirement for fat grafts in diabetes patients is expected to increase continuously. However, the circulation complications of diabetes are serious and have been shown to involve microvascular problems, impairing ischemia-driven neovascularization in particular. After injection, revascularization is vital to the survival of the grafted fat. In this study, the authors attempted to determine whether the diabetic condition inhibits the survival of injected fat due to impaired neovascularization.
Methods The rat scalp was used for testing fat graft survival. Forty-four seven-week-old male Sprague-Dawley rats were allocated to a diabetic group or a control group. 1.0 mL of processed fat was injected subcutaneously into the scalp of each rat. The effect of diabetes was evaluated by calculating the volume and the weight of the grafted fat and by histologically analyzing the fat sections.
Results The surviving fat graft volume and weight were considerably smaller in the diabetic group than in the control group (P<0.05), and histological evaluations showed less vascularity, and more cysts, vacuoles, and fibrosis in the diabetic group (P<0.05). Cellular integrity and inflammation were not considerably different in the two groups.
Conclusions As the final outcome, we found that the presence of diabetes might impair the survival and the quality of fat grafts, as evidenced by lower fat graft weights and volumes and poor histologic graft quality.
Publikationsverlauf
Eingereicht: 25. Juli 2013
Angenommen: 17. Oktober 2013
Artikel online veröffentlicht:
02. Mai 2022
© 2014. The Korean Society of Plastic and Reconstructive Surgeons. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonCommercial License, permitting unrestricted noncommercial use, distribution, and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes. (https://creativecommons.org/licenses/by-nc/4.0/)
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References
- 1 Ramon Y, Shoshani O, Peled IJ. et al. Enhancing the take of injected adipose tissue by a simple method for concentrating fat cells. Plast Reconstr Surg 2005; 115: 197-201
- 2 Langer S, Sinitsina I, Biberthaler P. et al. Revascularization of transplanted adipose tissue: a study in the dorsal skinfold chamber of hamsters. Ann Plast Surg 2002; 48: 53-59
- 3 Karacaoglu E, Kizilkaya E, Cermik H. et al. The role of recipient sites in fat-graft survival: experimental study. Ann Plast Surg 2005; 55: 63-68
- 4 Nguyen A, Pasyk KA, Bouvier TN. et al. Comparative study of survival of autologous adipose tissue taken and transplanted by different techniques. Plast Reconstr Surg 1990; 85: 378-386
- 5 Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010; 87: 4-14
- 6 Capla JM, Grogan RH, Callaghan MJ. et al. Diabetes impairs endothelial progenitor cell-mediated blood vessel formation in response to hypoxia. Plast Reconstr Surg 2007; 119: 59-70
- 7 Por YC, Yeow VK, Louri N. et al. Platelet-rich plasma has no effect on increasing free fat graft survival in the nude mouse. J Plast Reconstr Aesthet Surg 2009; 62: 1030-1034
- 8 Yang G, Luo C, Yan X. et al. Extracorporeal shock wave treatment improves incisional wound healing in diabetic rats. Tohoku J Exp Med 2011; 225: 285-292
- 9 Oh DS, Cheon YW, Jeon YR. et al. Activated platelet-rich plasma improves fat graft survival in nude mice: a pilot study. Dermatol Surg 2011; 37: 619-625
- 10 Teimourian B. Repair of soft-tissue contour deficit by means of semiliquid fat graft. Plast Reconstr Surg 1986; 78: 123-124
- 11 Coleman SR. Structural fat grafting. Aesthet Surg J 1998; 18: 386-388
- 12 Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 2001; 107: 229-237
- 13 Locke MB, de Chalain TM. Current practice in autologous fat transplantation: suggested clinical guidelines based on a review of recent literature. Ann Plast Surg 2008; 60: 98-102
- 14 Del Vecchio D, Rohrich RJ. A classification of clinical fat grafting: different problems, different solutions. Plast Reconstr Surg 2012; 130: 511-522
- 15 Peer LA. Cell survival theory versus replacement theory. Plast Reconstr Surg (1946) 1955; 16: 161-168
- 16 Shoshani O, Livne E, Armoni M. et al. The effect of interleukin-8 on the viability of injected adipose tissue in nude mice. Plast Reconstr Surg 2005; 115: 853-859
- 17 Jazayeri L, Callaghan MJ, Grogan RH. et al. Diabetes increases p53-mediated apoptosis following ischemia. Plast Reconstr Surg 2008; 121: 1135-1143
- 18 Centers for Disease Control and Prevention (CDC). Diabetes-related amputations of lower extremities in the Medicare population: Minnesota, 1993-1995. MMWR Morb Mortal Wkly Rep 1998; 47: 649-652
- 19 Baumgartner-Parzer SM, Wagner L, Pettermann M. et al. High-glucose: triggered apoptosis in cultured endothelial cells. Diabetes 1995; 44: 1323-1327
- 20 Quagliaro L, Piconi L, Assaloni R. et al. Intermittent high glucose enhances apoptosis related to oxidative stress in human umbilical vein endothelial cells: the role of protein kinase C and NAD(P)H-oxidase activation. Diabetes 2003; 52: 2795-2804
- 21 The Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications Research Group. Retinopathy and nephropathy in patients with type 1 diabetes four years after a trial of intensive therapy. N Engl J Med 2000; 342: 381-389
- 22 Shoshani O, Shupak A, Ullmann Y. et al. The effect of hyperbaric oxygenation on the viability of human fat injected into nude mice. Plast Reconstr Surg 2000; 106: 1390-1396
- 23 Ullmann Y, Hyams M, Ramon Y. et al. Enhancing the survival of aspirated human fat injected into nude mice. Plast Reconstr Surg 1998; 101: 1940-1944