Download PDF
CC BY-NC 4.0 · Arch Plast Surg 2012; 39(02): 106-112
DOI: 10.5999/aps.2012.39.2.106
Original Article

The Effect of Platelet-rich Plasma on Wounds of OLETF Rats Using Expression of Matrix Metalloproteinase-2 and -9 mRNA

Ho Seong Shin
Department of Plastic and Reconstructive Surgery, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine, Bucheon, Korea
,
Hwa Young Oh
Department of Plastic and Reconstructive Surgery, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine, Bucheon, Korea
› Author AffiliationsThis article has been adapted from Ho Seong Shin's dissertation submitted to Soonchunytang University Graduate School for the Ph.D. in Plastic Surgery.
› Further Information
   Permissions and Reprints

Background Complicated diabetic patients show impaired, delayed wound healing caused by multiple factors. A study on wound healing showed that platelet-rich plasma (PRP) was effective in normal tissue regeneration. Nonetheless, there is no evidence that when plateletrich plasma is applied to diabetic wounds, it normalizes the diabetic wound healing process. In this study, we have analyzed matrix metalloproteinase (MMP)-2, MMP-9 expression to investigate the effect of PRP on diabetic wounds.

Methods Twenty-four-week-old male Otsuka Long-Evans Tokushima Fatty rats were provided by the Tokushima Research Institute. At 50 weeks, wounds were arranged in two sites on the lateral paraspinal areas. Each wound was treated with PRP gel and physiologic saline gauze. To determine the expression of MMP-2, MMP-9, which was chosen as a marker of wound healing, reverse transcription polymerase chain reaction (RT-PCR) was performed and local distribution and expression of MMP-2, MMP-9 was also observed throughout the immunohistochemical staining.

Results RT-PCR and the immunohistochemical study showed that the levels of MMP-2, MMP-9 mRNA expression in PRP applied tissues were higher than MMP-2, MMP-9 mRNA expression in saline-applied tissues. MMP-9 mRNA expression in wounds of diabetic rats decreased after healing began to occur. But no statistical differences were detected on the basis of body weight or fasting blood glucose levels.

Conclusions This study could indicate the extracellular matrix-regulating effect observed with PRP. Our results of the acceleration of wound healing events by PRP under hyperglycemic conditions might be a useful clue for future clinical treatment for diabetic wounds.

Keywords

Platelet-rich plasma - Rats, OLETF - Matrix mtalloproteinase-2 - Matrix metalloproteinase-9

This article was presented at the 68th Congress of The Korean Society of Plastic and Reconstructive Surgeons on November 4, 2010 in Seoul, Korea.




Publication History

Received: 01 October 2011

Accepted: 02 November 2011

Article published online:
24 April 2022

© 2012. The Korean Society of Plastic and Reconstructive Surgeons. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonCommercial License, permitting unrestricted noncommercial use, distribution, and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes. (https://creativecommons.org/licenses/by-nc/4.0/)

Thieme Medical Publishers, Inc.
333 Seventh Avenue, 18th Floor, New York, NY 10001, USA

 

  • REFERENCES

  • 1 Falanga V. Wound healing and its impairment in the diabetic foot. Lancet 2005; 366: 1736-1743
    CrossrefPubMedGoogle Scholar
  • 2 Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999; 14: 529-535
    Google Scholar
  • 3 Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 2004; 62: 489-496
    CrossrefPubMedGoogle Scholar
  • 4 Madlener M, Parks WC, Werner S. Matrix metalloproteinases (MMPs) and their physiological inhibitors (TIMPs) are differentially expressed during excisional skin wound repair. Exp Cell Res 1998; 242: 201-210
    CrossrefPubMedGoogle Scholar
  • 5 Kawano K, Hirashima T, Mori S. et al. Spontaneous long-term hyperglycemic rat with diabetic complications. Otsuka Long-Evans Tokushima Fatty (OLETF) strain. Diabetes 1992; 41: 1422-1428
    CrossrefPubMedGoogle Scholar
  • 6 Simman R, Hoffmann A, Bohinc RJ. et al. Role of platelet-rich plasma in acceleration of bone fracture healing. Ann Plast Surg 2008; 61: 337-344
    CrossrefPubMedGoogle Scholar
  • 7 Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg 2001; 107: 229-237
    CrossrefPubMedGoogle Scholar
  • 8 Della Valle A, Sammartino G, Marenzi G. et al. Prevention of postoperative bleeding in anticoagulated patients undergoing oral surgery: use of platelet-rich plasma gel. J Oral Maxillofac Surg 2003; 61: 1275-1278
    CrossrefPubMedGoogle Scholar
  • 9 Blanton MW, Hadad I, Johnstone BH. et al. Adipose stromal cells and platelet-rich plasma therapies synergistically increase revascularization during wound healing. Plast Reconstr Surg 2009; 123: 56S-64S
    CrossrefPubMedGoogle Scholar
  • 10 Marx RE, Carlson ER, Eichstaedt RM. et al. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998; 85: 638-646
    CrossrefPubMedGoogle Scholar
  • 11 Margolis DJ, Kantor J, Santanna J. et al. Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers. Diabetes Care 2001; 24: 483-488
    CrossrefPubMedGoogle Scholar
  • 12 Medina A, Scott PG, Ghahary A. et al. Pathophysiology of chronic nonhealing wounds. J Burn Care Rehabil 2005; 26: 306-319
    CrossrefPubMedGoogle Scholar
  • 13 Liu Y, Min D, Bolton T. et al. Increased matrix metalloproteinase-9 predicts poor wound healing in diabetic foot ulcers. Diabetes Care 2009; 32: 117-119
    CrossrefPubMedGoogle Scholar
  • 14 Lan CC, Liu IH, Fang AH. et al. Hyperglycaemic conditions decrease cultured keratinocyte mobility: implications for impaired wound healing in patients with diabetes. Br J Dermatol 2008; 159: 1103-1115
    PubMedGoogle Scholar
  • 15 Lund LR, Romer J, Bugge TH. et al. Functional overlap between two classes of matrix-degrading proteases in wound healing. EMBO J 1999; 18: 4645-4656
    CrossrefPubMedGoogle Scholar
  • 16 Wall SJ, Bevan D, Thomas DW. et al. Differential expression of matrix metalloproteinases during impaired wound healing of the diabetes mouse. J Invest Dermatol 2002; 119: 91-98
    CrossrefPubMedGoogle Scholar