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DOI: 10.4103/ijmpo.ijmpo_217_17
Eosinophil Density in Common Benign and Malignant Salivary Gland Tumors with Congo Red Staining
Financial support and sponsorship This study was supported by Babol Medical University.![](https://www.thieme-connect.de/media/10.1055-s-00050348/201902/lookinside/thumbnails/10-4103-ijmpo-ijmpo_217_17_212-1.jpg)
Abstract
Purpose: Eosinophils are multifunctional leukocytes with unknown mechanisms in salivary gland tumors. Some researchers associate eosinophils with tumor progression and others have proposed them as antitumoral. The present study was conducted to compare the density of eosinophils in common salivary gland tumors and its relationship with the degree of differentiation in malignant salivary gland tumors. Materials and Methods: In this present descriptive-analytical, cross-sectional study, 15 cases of pleomorphic adenoma, 15 cases of adenoid cystic carcinoma (ACC), 30 cases of mucoepidermoid carcinoma (MEC), and 5 cases of normal salivary glands were extracted. Sections were prepared of these cases for Congo red staining. The malignant salivary gland tumors were classified as high-grade and low-grade malignancies. The slides were observed under ×10, and the highest-density areas were selected and counted in ten microscopic fields under ×40. Results: The density of the eosinophils was 4.5 ± 5.6 in the pleomorphic adenoma, 16.2 ± 6.01 in the low-grade MEC, 1.05 ± 1.1 in the high-grade MEC (Grade III), and the ACC, and 0.4 ± 0.89 in the normal salivary gland. Eosinophil density was significantly higher in low-grade malignancies compared to in benign or high-grade malignant neoplasms and normal salivary gland (P < 0.001). Conclusion: This is suggested which the density of eosinophils is associated with the process of tumorigenesis and the degree of malignancy in malignant salivary gland tumors.
Keywords
Adenoid cystic - carcinoma - Congo red - eosinophil - mucoepidermoid carcinoma - pleomorphic adenomaPublication History
Article published online:
03 June 2021
© 2019. Indian Society of Medical and Paediatric Oncology. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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References
- 1 Sando Z, Fokouo JV, Mebada AO, Djomou F, NDjolo A, Oyono JL. et al. Epidemiological and histopathological patterns of salivary gland tumors in cameroon. Pan Afr Med J 2016; 23: 66
- 2 Nagamine K, Kitamura T, Yanagawa-Matsuda A, Ohiro Y, Tei K, Hida K. et al. Expression of parathyroid hormone-related protein confers malignant potential to mucoepidermoid carcinoma. Oncol Rep 2013; 29: 2114-8
- 3 Ata-Ali J, Zurriaga O, Alberich C. Incidence and survival rates for malignant salivary gland tumors. J Oral Sci 2016; 58: 67-73
- 4 Pires FR, Pringle GA, de Almeida OP, Chen SY. Intra-oral minor salivary gland tumors: A clinicopathological study of 546 cases. Oral Oncol 2007; 43: 463-70
- 5 Boukheris H, Curtis RE, Land CE, Dores GM. Incidence of carcinoma of the major salivary glands according to the WHO classification, 1992 to 2006: A population-based study in the United States. Cancer Epidemiol Biomarkers Prev 2009; 18: 2899-906
- 6 Jain M, Kasetty S, Sudheendra US, Tijare M, Khan S, Desai A. et al. Assessment of tissue eosinophilia as a prognosticator in oral epithelial dysplasia and oral squamous cell carcinoma-an image analysis study. Patholog Res Int 2014; 2014: 507512
- 7 Gomes P, Torres SM, Plager DA, Jessen CR, Lee JJ. Comparison of three staining methods to identify eosinophils in formalin-fixed canine skin. Vet Dermatol 2013; 24: 323-8, e71-2
- 8 Sahni P, Patel A, Md S, Hallur J, Gujjar PK. Tumor associated tissue eosinophilia in oral squamous cell carcinoma: A histo-chemical analysis. Malays J Med Sci 2015; 22: 21-5
- 9 Saravani SH, Kadeh H, Derakhshanfar GH. Tumor-associated tissue eosinophilia in squamous cell carcinoma of head and neck. Dent Clin Exp 2015; 1: e5634
- 10 Yellapurkar S, Natarajan S, Boaz K, Baliga M, Shetty P, Manaktala N. et al. Tumour-associated tissue eosinophilia in oral squamous cell carcinoma – A boon or a bane?. J Clin Diagn Res 2016; 10: ZC65-8
- 11 Brandwein MS, Ferlito A, Bradley PJ, Hille JJ, Rinaldo A. Diagnosis and classification of salivary neoplasms: Pathologic challenges and relevance to clinical outcomes. Acta Otolaryngol 2002; 122: 758-64
- 12 Regezi J, Sciubba J, Gordan CK. Oral Pathology, Clinical Pathology Correlations. California: W.B. Saunders Co.; 2008
- 13 Jain M, Kasetty S, Khan S, Jain NK. Tissue eosinophilia in head and neck squamous neoplasia: An update. Exp Oncol 2014; 36: 157-61
- 14 Radhi J, Otero C, Reid S. Significant eosinophilic infiltrate of the breast. Int J Pathol 2008; 9: 1-5
- 15 Lorena SC, Dorta RG, Landman G, Nonogaki S, Oliveira DT. Morphometric analysis of the tumor associated tissue eosinophilia in the oral squamous cell carcinoma using different staining techniques. Histol Histopathol 2003; 18: 709-13
- 16 Davis BP, Rothenberg ME. Eosinophils and cancer. Cancer Immunol Res 2014; 2: 1-8
- 17 Gatault S, Legrand F, Delbeke M, Loiseau S, Capron M. Involvement of eosinophils in the anti-tumor response. Cancer Immunol Immunother 2012; 61: 1527-34
- 18 Joshi PS, Kaijkar MS. A histochemical study of tissue eosinophilia in oral squamous cell carcinoma using Congo red staining. Dent Res J (Isfahan) 2013; 10: 784-9
- 19 Costello R, O'Callaghan T, Sébahoun G. Eosinophils and antitumour response. Rev Med Interne 2005; 26: 479-84
- 20 Martinelli-Kläy CP, Mendis BR, Lombardi T. Eosinophils and oral squamous cell carcinoma: A short review. J Oncol 2009; 2009: 310132
- 21 Muniz VS, Weller PF, Neves JS. Eosinophil crystalloid granules: Structure, function, and beyond. J Leukoc Biol 2012; 92: 281-8
- 22 Tadbir AA, Ashraf MJ, Sardari Y. Prognostic significance of stromal eosinophilic infiltration in oral squamous cell carcinoma. J Craniofac Surg 2009; 20: 287-9
- 23 Alkhabuli JO, High AS. Significance of eosinophil counting in tumor associated tissue eosinophilia (TATE). Oral Oncol 2006; 42: 849-50