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CC BY-NC-ND 4.0 · Eur J Dent 2018; 12(02): 287-291
DOI: 10.4103/ejd.ejd_9_18
Original Article
European Journal of Dentistry

Assessment of cell viability in four novel endodontic sealers

Vassiliki Taraslia
1   Department of Genetics and Gene Therapy, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
,
Ema Anastasiadou
1   Department of Genetics and Gene Therapy, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
,
Christina Lignou
1   Department of Genetics and Gene Therapy, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
,
Georgios Keratiotis
2   Department of Endodontics, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
,
Anastasia Agrafioti
2   Department of Endodontics, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
,
Evangelos G. Kontakiotis
2   Department of Endodontics, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece
› Author Affiliations
Further Information

Publication History

Publication Date:
16 September 2019 (online)

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ABSTRACT

Objective: The aim of this study was to evaluate the viability of human periodontal ligament (PDL) cells on MTA-Fillapex, GuttaFlow 2, TotalFill Sealer, and BioRootTM RCS in comparison to conventional epoxy resin-based (AH Plus) and zinc-oxide-eugenol-based (Roth’s 801) sealers. Materials and Methods: Sealers were divided into two groups, and five coverslips for each material per group were prepared. In the first group, PDLs were added immediately after the preparation of sealers (Fresh Group), and in the second, PDLs were added after 24 h. PDLs were cultured for 72 h and afterward, counted using standard hematocytometry. A Mann–Whitney U-test and Kruskal–Wallis test were used for the statistical analysis. The level of significance was set at 5%. Furthermore, cell morphology was assessed by confocal microscopy. Results: The number of viable cells for the 24 h-set groups was higher than the freshly mixed in all sealers except Roth’s 801. In both groups, GuttaFlow 2 presented the highest number of viable cells. In a descending order of cells’ survival, TotalFill, BioRoot, and MTA-Fillapex are following and the conventional sealers, AH Plus and Roth’s 801, seem not to exhibit the biological properties of the others. Cells grown on GuttaFlow 2, TotalFill, and BioRoot were observed to be well-formed. In contrast, MTA-Fillapex exhibited untypical morphology. No cells were detected on the surfaces of AH Plus, as well as Roth’s 801. Conclusions: All novel sealers presented increased cell viability in comparison to conventional sealers. GuttaFlow 2 exhibited the highest cell viability.

Key words:

Cell morphology - cells viability - sealers
 

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