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CC BY-NC-ND 4.0 · Eur J Dent 2018; 12(03): 358-362
DOI: 10.4103/ejd.ejd_342_17
Original Article
Dental Investigation Society

A potential therapy of human umbilical cord mesenchymal stem cells for bone regeneration on osteoporotic mandibular bone

Nike Hendrijantini
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Tuti Kusumaningsih
2   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Rostiny Rostiny
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Pungky Mulawardhana
3   Department of Obstetrics and Gynecology, Medical Faculty, Universitas Airlangga, Surabaya, Indonesia
4   Department of Obstetrics and Gynecology, Dr. Soetomo Hospital, Surabaya, Indonesia
,
Coen Pramono Danudiningrat
5   Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fedik Abdul Rantam
6   Department of Microbiology and Virology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations
Further Information

Publication History

Publication Date:
16 September 2019 (online)

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ABSTRACT

Objective: The aim of this study is to prove that human umbilical cord mesenchymal stem cell (hUCMSC) therapy on mandibular osteoporotic model is able to increase transforming growth factor-beta-1 (TGF)-β1 expression, Runx2, and osteoblasts. Materials and Methods: This research is true experimental posttest control group design. Thirty female Wistar rats were divided into 6 groups randomly, which consisted of sham surgery for control (T1), ovariectomy as osteoporotic group (T2), osteoporotic group injected with gelatine for 4 weeks (T3), 8 weeks (T4) injected with hUCMSC-gelatine for 4 weeks (T5) and 8 weeks (T6). All mice were presented for immunohistochemistry examination for TGF-β1, Runx2, and histology for osteoblasts. Results: The lowest level of osteoblast was osteoporotic group injected with gelatine in 4 weeks compared to other groups. There were increases of TGF-β1, Runx2, and osteoblasts from osteoporotic group compared to osteoporotic post-hUCMSC-gelatine injection group. Conclusion: The hUCMSC has a high osteogenic effect and increases the osteoporotic mandibular bone regeneration on the animal model that is showed by the increase of the level of TGF-β1, Runx2, and osteoblasts.

Key words:

Human umbilical cord mesenchymal stem cells - mandibular - osteoporosis
 

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