Download PDF
CC BY-NC-ND 4.0 · J Lab Physicians 2018; 10(04): 437-442
DOI: 10.4103/JLP.JLP_157_17
Original Article

Evaluation of diagnostic utility of imprint cytology in paediatric renal tumours with special references to Ki 67 proliferative marker

Namrata Maity
Department of Pathology, IPGME and R, Apollo Gleneagles Hospital, Calcutta National Medical College and Hospital, Kolkata, West Bengal, India
,
Chhanda Das
Department of Pathology, IPGME and R, Apollo Gleneagles Hospital, Calcutta National Medical College and Hospital, Kolkata, West Bengal, India
,
Madhumita Mukhopadhyay
Department of Pathology, IPGME and R, Apollo Gleneagles Hospital, Calcutta National Medical College and Hospital, Kolkata, West Bengal, India
,
Tamanna Parvin
Department of Pathology, IPGME and R, Apollo Gleneagles Hospital, Calcutta National Medical College and Hospital, Kolkata, West Bengal, India
,
Ashis Kumar Saha
Department of Surgery, Calcutta National Medical College and Hospital, Kolkata, West Bengal, India
,
Biswanath Mukhopadhyay
Department of Paediatric Surgery, Apollo Gleneagles Hospital, Calcutta National Medical College and Hospital, Kolkata, West Bengal, India
› Author Affiliations Financial support and sponsorship Nil.
› Further Information
  PDF Download Permissions and Reprints

ABSTRACT

BACKGROUND: Pediatric renal neoplasms comprise about 7%–8% of all neoplasms in children. Wilms tumour (WT) is the most common among pediatric renal tumours.

AIMS AND OBJECTIVES: The study was undertaken to study the epidemiological occurrence of pediatric renal tumours in a tertiary care hospital and to ascertain the validity and reliability of touch smear imprint cytology in intraoperative diagnosis of renal tumours and correlate with subsequent histopathological diagnosis and to assess the expression of proliferation marker Ki-67 in different components and stages of WT.

MATERIALS AND METHODS: It was a single-institution-based prospective and observational study, conducted for 2 years (from October 2013 to September 2015) in the department of pathology at our hospital. A total of fifty cases were enrolled in this study, all were below 15 years of age.

RESULTS: Imprint cytology showed sensitivity, specificity, and diagnostic accuracy of 83%, 98%, and 95.74%, respectively, in diagnosing benign and malignant renal tumours. There was statistically significant correlation of imprint cytology with confirmatory histopathological examination of excision specimen (P < 0.001). Immunohistochemical analysis of Ki-67 was done in all WT cases. Epithelial component had higher proliferative index than blastemal component with P = 0.0082, which was highly statistically significant.

CONCLUSION: Imprint cytology is found to be a less expensive, simple, and rapid method, which can be used as an adjunct to histopathology. Correlation between proliferation index as measured with Ki-67 antibody and tumour stage was found. Ki-67 is thus a relevant marker for assessing the proliferative activity.

Keywords

Imprint cytology - kidney tumour - proliferative index


Publication History

Received: 16 October 2017

Accepted: 30 May 2018

Article published online:
20 February 2020

© 2018.

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Sebire NJ, Vujanic GM. Paediatric renal tumours: Recent developments, new entities and pathological features. Histopathology 2009;54:516-28.
  • 2 Murphy WM, Grignon DJ, Perlman EJ. Kidney tumours in children. In: AFIP Atlas of Tumour Pathology 4th Series Fascicle 1: Tumours of the Kidney, Bladder and Related Urinary Structures. Washington DC: American Registry of Pathology; 2004.
  • 3 van den Heuvel-Eibrink MM, Grundy P, Graf N, Pritchard-Jones K, Bergeron C, Patte C, et al. Characteristics and survival of 750 children diagnosed with a renal tumour in the first seven months of life: A collaborative study by the SIOP/GPOH/SFOP, NWTSG, and UKCCSG Wilms tumour study groups. Pediatr Blood Cancer 2008;50:1130-4.
  • 4 Gupta DK. Progress in treatment of Wilms’ tumour. In: Hemal AK, editor. Advances in Uro-Oncology. New Delhi: Saurabh Publishers; 1995.
  • 5 Gupta DK, Sharma S, Agarwala S, Carachi R. Saga of Wilms’ tumour –Lessons learnt from the past. (Department of Pediatric Surgery, All India Institute of Medical Sciences, New Delhi and *York Hill Children’s Hospital, Glasgow, U.K.). J Indian Assoc Pediatr Surg 2005;10:217-28.
  • 6 Suen KC, Wood WS, Syed AA, Quenville NF, Clement PB. Role of imprint cytology in intraoperative diagnosis: Value and limitations. J Clin Pathol 1978;31:328-37.
  • 7 Hajdu SI, Melamed MR. Limitations of aspiration cytology in the diagnosis of primary neoplasms. Acta Cytol 1984;28:337-45.
  • 8 Wen JG, van Steenbrugge GJ, Egeler RM, Nijman RM. Progress of fundamental research in Wilms’ tumour. Urol Res 1997;25:223-30.
  • 9 Cattoretti G, Becker MH, Key G, Duchrow M, Schlüter C, Galle J, et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 1992;168:357-63.
  • 10 Ghanem MA, Van der Kwast TH, Sudaryo MK, Mathoera RB, van den Heuvel MM, Al-Doray AA, et al. MIB-1 (KI-67) proliferation index and cyclin-dependent kinase inhibitor p27(Kip1) protein expression in nephroblastoma. Clin Cancer Res 2004;10:591-7.
  • 11 Vujanić GM, Sandstedt B, Harms D, Kelsey A, Leuschner I, de Kraker J, et al. Revised International Society of Paediatric Oncology (SIOP) working classification of renal tumours of childhood. Med Pediatr Oncol 2002;38:79-82.
  • 12 Breslow N, Olshan A, Beckwith JB, Green DM. Epidemiology of Wilms tumour. Med Pediatr Oncol 1993;21:172-81.
  • 13 Breslow N, Beckwith JB, Ciol M, Sharples K. Age distribution of Wilms’ tumour: Report from the national Wilms’ tumour study. Cancer Res 1988;48:1653-7.
  • 14 Blute ML, Kelalis PP, Offord KP, Breslow N, Beckwith JB, D’Angio GJ, et al. Bilateral Wilms tumour. J Urol 1987;138:968-73.
  • 15 Charles AK, Vujanić GM, Berry PJ. Renal tumours of childhood. Histopathology 1998;32:293-309.
  • 16 Shet T, Viswanathan S. The cytological diagnosis of paediatric renal tumours. J Clin Pathol 2009;62:961-9.
  • 17 Vujanić GM, Sandstedt B. The pathology of Wilms’ tumour (nephroblastoma): The international society of paediatric oncology approach. J Clin Pathol 2010;63:102-9.
  • 18 Jurić I, Pogorelić Z, Kuzmić-Prusac I, Biocić M, Jakovljević G, Stepan J, et al. Expression and prognostic value of the Ki-67 in Wilms’ tumour: Experience with 48 cases. Pediatr Surg Int 2010;26:487-93.