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CC BY-NC-ND 4.0 · European Journal of General Dentistry 2012; 1(03): 192-196
DOI: 10.4103/2278-9626.105386
Original Article

Comparison of centrifuged liquid based cytology method with conventional brush cytology in oral lesions

Nidhi Dwivedi
Department of Oral Pathology and Microbiology, Saraswati Dental College and Hospital, Lucknow, Uttar Pradesh, India
,
Akhil Agarwal
1   Department of Orthodontics and Dentofacial Orthopedics, Babu Banarsi Das College of Dental Sciences, Lucknow, Uttar Pradesh, India
,
Vineet Raj
Department of Oral Pathology and Microbiology, Saraswati Dental College and Hospital, Lucknow, Uttar Pradesh, India
,
Bina Kashyap
Department of Oral Pathology and Microbiology, Saraswati Dental College and Hospital, Lucknow, Uttar Pradesh, India
,
Shaleen Chandra
Department of Oral Pathology and Microbiology, Saraswati Dental College and Hospital, Lucknow, Uttar Pradesh, India
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Abstract

Background: Exfoliative cytology is the study of cells that are shed or scrapped off from mucosal surfaces. Centrifuged Liquid based cytology is a modified technique employed in the present study. Aims: To compare the utility of centrifuged liquid based cytology with conventional cytology in oral lesions after staining with Papanicolaou (PAP) stain. Materials and Methods: 50 cases of oral lesions comprising of normal mucosa (n=14), hyperkeratotic lesions (n=17), ulcerated lesions (n=7) and atrophic lesions (n=12) were selected. Two smears were obtained from the lesion using a cytological brush. One was spread on the slide using conventional technique, fixed immediately in 95% ethyl alcohol. Second sample was suspended in suspending solution for 10 minutes then spun in centrifuge for 10 minutes. The supernatant was poured off and the obtained cell pellet was used to prepare a smear by sedimentation and left to dry overnight. Both the smears were stained by PAP. The stained smears were compared for seven morphological parameters such as adequacy of smear, clear background, cell distribution, smear thickness, cell morphology, and presence of blood, inflammatory cells, microbial colonies and artifacts. Wilcoxon Signed rank test was used and P≤0.05 was considered statistically significant. Results: There was a statistically significant difference (P<0.001) between centrifuged liquid based cytology and conventional cytology when clear background was evaluated while in all other parameters the difference was not significant. Conclusion: Centrifuged Liquid based cytology showed clearer background than conventional brush cytology in oral lesions.

Keywords

Brush cytology - liquid based cytology - oral lesions - PAP


Publication History

Article published online:
01 November 2021

© 2012. European Journal of General Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/.)

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