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CC BY-NC-ND 4.0 · Avicenna J Med 2017; 07(01): 12-16
DOI: 10.4103/2231-0770.197508
ORIGINAL ARTICLE

Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital

Trupti Bajpai
Department of Microbiology, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh; Department of Biochemistry, IGNOU, SOS, New Delhi, India
Department of Biochemistry, IGNOU, SOS, New Delhi, India
,
M Pandey
Department of Biochemistry, IGNOU, SOS, New Delhi, India
,
M Varma
Department of Biochemistry, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh, India
,
G S Bhatambare
Department of Microbiology, Sri Aurobindo Institute of Medical Sciences Medical College and PG Institute, Indore, Madhya Pradesh; Department of Biochemistry, IGNOU, SOS, New Delhi, India
› Institutsangaben Financial support and sponsorship Nil.
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Abstract

Introduction: Extended-spectrum beta-lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as penicillins, cephalosporins, and monobactams. They are derived from the narrow-spectrum beta-lactamases (TEM-1, TEM-2, or SHV-1) by mutations that alter the amino acid configuration around the enzyme active site. Aim: To determine the prevalence of ESBL (bla TEM , bla CTX-M , and bla SHV ) genes among the members of Enterobacteriaceae. Methodology: The present prospective study was carried out from January 2015 to June 2015 in the Department of Microbiology and Molecular Medicine of a Teaching Tertiary Care Hospital. A total of 526 urine samples were studied. Seventy-eight isolates were subjected to polymerase chain reaction for detection of ESBL genes. Results: In our study, ESBL genes were detected among 18 (45%) phenotypically confirmed ESBL producers and 20 (52.5%) phenotypically confirmed non-ESBL producers. The gene that predominated was bla TEM (48.7%), followed by bla CTX-M (7.6%) and bla SHV (5.1%). Conclusion: Definitive identification of ESBL genes is only possible by molecular detection methods. Phenotypic tests need to be evaluated periodically as their performance may change with the introduction of new enzymes.

Keywords

Beta-lactam antibiotics - extended-spectrum beta-lactamases - polymerase chain reaction


Publikationsverlauf

Artikel online veröffentlicht:
09. August 2021

© 2017. Syrian American Medical Society. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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