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CC BY-NC-ND 4.0 · World J Nucl Med 2017; 16(01): 45-50
DOI: 10.4103/1450-1147.181155
Original article

99mTc-Tetrofosmin uptake correlates with the sensitivity of glioblastoma cell lines to temozolomide

Authors

  • George Alexiou

    Neurosurgical Institute, University of Ioannina, Ioannina

  • Xanthi Xourgia

    1   Department of Nuclear Medicine, University Hospital of Ioannina, Ioannina

  • Paraskevi Gerogianni

    Neurosurgical Institute, University of Ioannina, Ioannina

  • Evrysthenis Vartholomatos

    Neurosurgical Institute, University of Ioannina, Ioannina

  • John Kalef-Ezra

    2   Department of Medical Physics, University of Ioannina, Ioannina

  • Andreas Fotopoulos

    1   Department of Nuclear Medicine, University Hospital of Ioannina, Ioannina

  • Athanasios Kyritsis

    Neurosurgical Institute, University of Ioannina, Ioannina
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99mTc-tetrofosmin (99mTc-TF) is a single-photon emission computed tomography tracer that has been used for brain tumor imaging. The aim of the study was to assess if 99mTc-TF uptake by glioblastoma cells correlates with their response to temozolomide (TMZ). We investigated the correlation of TMZ antitumor effect with the 99mTc-TF uptake in two glioblastoma cell lines. The U251MG cell line is sensitive to TMZ, whereas T98G is resistant. Viability and proliferation of the cells were examined by trypan blue exclusion assay and xCELLigence system. Cell cycle was analyzed with flow cytometry. The radioactivity in the cellular lysate was measured with a gamma scintillation counter. TMZ induced G2/M cell cycle arrest in U251MG cells, whereas there was no effect on cell cycle in T98G cells. Lower 99mTc-TF uptake was observed in U251MG cells that were exposed to TMZ compared to control (P = 0.0159). No significant difference in respect to 99mTc-TF uptake was found in T98G cells when exposed to TMZ compared to control (P = 0.8). With 99mTc-TF, it was possible to distinguish between TMZ-sensitive and resistant glioblastoma cells within 6 h of treatment initiation. Thus, 99mTc-TF uptake may consist a novel approach to assess an early response of glioblastoma to chemotherapy and deserves further investigation.

Keywords

99mTc-tetrofosmin - glioma - temozolomide


Publication History

Article published online:
18 May 2022

© 2017. Sociedade Brasileira de Neurocirurgia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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