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CC BY-NC-ND 4.0 · Eur J Dent 2016; 10(03): 345-350
DOI: 10.4103/1305-7456.184163
Original Article
Dental Investigation Society

Frequency of sucrose exposure on the cariogenicity of a biofilm-caries model

Natalia Díaz-Garrido
1   Department of Oral Rehabilitation, Cariology Unit, University of Talca, Talca, Chile
,
Carla Lozano
2   Oral Biology and Biochemistry Laboratory, Institute for Research in Dental Sciences, Faculty of Dentistry, University of Chile, Santiago, Chile
,
Rodrigo A. Giacaman
1   Department of Oral Rehabilitation, Cariology Unit, University of Talca, Talca, Chile
3   Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), University of Talca, Talca, Chile
› Author Affiliations
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Publication History

Publication Date:
24 September 2019 (online)

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ABSTRACT

Objective: Although sucrose is considered the most cariogenic carbohydrate in the human diet, the question of how many exposures are needed to induce damage on the hard dental tissues remains unclear. To approach this question, different frequencies of daily sucrose exposure were tested on a relevant biological caries model. Materials and Methods: Biofilms of the Streptococcus mutans were formed on enamel slabs and exposed to cariogenic challenges with 10% sucrose for 5 min at 0, 1, 3, 5, 8, or 10 times per day. After 5 days, biofilms were retrieved to analyze biomass, protein content, viable bacteria, and polysaccharide formation. Enamel demineralization was evaluated by percentage of microhardness loss (percentage surface hardness loss [%SHL]). Results: Biomass, protein content, polysaccharide production, acidogenicity of the biofilm, and %SHL proportionally increased with the number of daily exposures to sucrose (P < 0.05). One daily sucrose exposure was enough to induce 20% more demineralization than the negative unexposed control. Higher frequencies induced greater demineralization and more virulent biofilms, but eight and ten exposures were not different between them in most of the analyzed variables (P > 0.05). Conclusions: Higher sucrose exposure seems to increase cariogenicity, in a frequency-dependent manner, by the modification of bacterial virulent properties.

Key words:

Caries - diet - nutrition - Streptococcus mutans - sucrose - sugar
 

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