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CC BY-NC-ND 4.0 · Eur J Dent 2016; 10(03): 341-344
DOI: 10.4103/1305-7456.184157
Original Article
Dental Investigation Society

Nestin expressions of exposed pulp after direct pulp capping by calcium hydroxide and platelet rich plasma

Sartika Puspita
1   Biomedical Department, School of Dentistry, Medical and Health Sciences Faculty Universitas Muhammadiyah Yogyakarta (UMY), Indonesia
,
Totok Utoro
2   Department of Pathology Anatomi, Faculty of Medicine, Gadjah Mada University, Yogyakarta, Indonesia
,
Tetiana Haniastuti
3   Department of Oral Biology, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia
› Author Affiliations
Further Information

Publication History

Publication Date:
24 September 2019 (online)

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ABSTRACT

Objective: The aim of this study was to evaluate nestin expression of pulp tissue following direct pulp capping with platelet-rich plasma (PRP). Materials and Methods: The thirty sound teeth from Sprague-Dawley rats were used and divided into two groups: Groups 1, teeth were capped with calcium hydroxide/Ca(OH)2 (n = 15) and Group 2 with PRP (n = 15). After 1st, 7th, and 21st days, respectively, 5 teeth each group (American Dental Association 41) were processed for light microscopic examination. Expressions of nestin were assessed by immunohistochemical techniques. Results: Nestin expression of Ca(OH)2 on the distance place of exposure at 1st and 7th days were 80% and at 21st day were 60%. Nestin expression of PRP on the distance place at 1st day was 80%, 7th 100%, and 21st day was 80%. At day 21 observation, Kruskal–Wallis test shows nestin expression was increased significantly in PRP groups (P < 0.05), but it was not increase significantly compare with Ca(OH)2. Conclusion: PRP had ability as a direct pulp capping material to induce nestin expression.

Key words:

Nestin expressions - platelet rich plasma - pulp capping
 

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