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CC BY-NC-ND 4.0 · Eur J Dent 2015; 09(04): 523-528
DOI: 10.4103/1305-7456.172613
Original Article
Dental Investigation Society

Efficacy of etidronic acid, BioPure MTAD and SmearClear in removing calcium ions from the root canal: An in vitro study

Hemant Kumar Yadav
1   Department of Conservative Dentistry and Endodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India
,
A. P. Tikku
2   Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, King George Medical University, Lucknow, Uttar Pradesh, India
,
Anil Chandra
2   Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, King George Medical University, Lucknow, Uttar Pradesh, India
,
Rakesh Kumar Yadav
2   Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, King George Medical University, Lucknow, Uttar Pradesh, India
,
Devendra Kumar Patel
3   Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR- Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh, India
› Author Affiliations
Further Information

Publication History

Publication Date:
23 September 2019 (online)

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ABSTRACT

Objective: The purpose of this study was to quantify the amount of calcium ions removed from the root canal by etidronic acid (HEBP), BioPure MTAD, and SmearClear using atomic absorption spectrophotometer. Materials and Methods: Fifty (n = 50) freshly extracted human mandibular premolar teeth were collected and decoronated at the cementoenamel junction. The canals were prepared in a crown down fashion using the rotary system and copiously irrigated with 1.0% sodium hypochlorite. All specimens were rinsed with the deionized water. Based on the type of chelating agent used, the samples (n = 10) were randomly divided into five (four test and one negative control) groups. Accordingly, Group I - 9% HEBP, Group II - 18% HEBP, Group III - SmearClear, Group IV - BioPure MTAD, and Group V - normal Saline. Subsequent to irrigation, the solution was collected in a test tube and subjected to atomic absorption spectrophotometer for the quantification of calcium ions removed from the root canal. Results: The mean concentration of calcium ions removed from the root canal (mean ± standard deviation) in all groups (I–V) were 13.32 ± 0.54 μg/ml, 16.36 ± 0.27 μg/ml, 20.04 ± 0.24 μg/ml, 18.15 ± 0.39 μg/ml, and 8.74 ± 0.49 μg/ml, respectively. Conclusions: SmearClear was the most effective agent for the removal of calcium ions from the root canal. Hence, its combined use with an organic solvent can be recommended for efficient smear layer removal.

Key words:

Atomic absorption spectrophotometry - BioPure MTAD - etidronic acid - SmearClear
 

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