CC BY-NC-ND 4.0 · Eur J Dent 2014; 08(04): 509-514
DOI: 10.4103/1305-7456.143634
Original Article
Dental Investigation Society

Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: An in vitro study

Liliana Guimarães Oliveira Ricatto
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
,
Luis Augusto Lopato Conrado
2   Department of Biomedical Engineering, Unicastelo, São José dos Campos, São Paulo, Brazil
,
Cecilia Pedroso Turssi
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
,
Fabiana Mantovani Gomes França
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
,
Roberta Tarkany Basting
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
,
Flávia Lucisano Botelho Amaral
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
› Institutsangaben
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Publikationsverlauf

Publikationsdatum:
25. September 2019 (online)

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ABSTRACT

Objective: The aim of this study was to evaluate in vitro the effect of photodynamic therapy (PDT) using LASER or light emitting diode (LED) on cariogenic bacteria (Streptococcus mutans [SM] and Lactobacillus casei [LC]) in bovine dentin. Materials and Methods: Twenty five fragments of dentin were contaminated with SM and LC strands and divided into five experimental groups according to the therapy they received (n = 5): C – control (no treatment), SCLED – no dye/LED application (94 J/cm2), SCLASER – no dye/LASER application (94 J/cm2), CCLED – dye/LED application (94 J/cm2) and CCLASER – dye/LASER application (94 J/cm2). The dye used was methylene blue at 10 mM. Dentin scrapes were harvested from each fragment and prepared for counts of colony forming units (CFU)/mL. The data were analyzed using Kruskal–Wallis, followed by Student–Newman–Keuls (⍺ =0.05). Results: Regarding SM, groups CCLASER and CCLED showed a significant reduction in CFU/mL, which was statistically superior to the SCLASER, SCLED and C groups. Regarding LC, the groups CCLASER and CCLED caused a significant reduction in CFU/mL when compared with SCLASER, which showed intermediate values. SCLED and C had a lesser effect on reducing CFU/mL, where the former showed values similar to those of SCLASER. Conclusions: In conclusion, PDT combined with LASER or LED and methylene blue had a significant antimicrobial effect on cariogenic bacteria in the dentin.