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CC BY-NC-ND 4.0 · Eur J Dent 2013; 07(04): 442-448
DOI: 10.4103/1305-7456.120683
Original Article
Dental Investigation Society

The effect of different root canal medicaments on the elimination of Enterococcus faecalis ex vivo

Till Dammaschke
1   Department of Operative Dentistry, Westphalian Wilhelms-University Münster, Germany
,
Nina Jung
2   Private Practice, Berlin, Germany
,
Inga Harks
3   Department of Periodontology, Westphalian Wilhelms-University, Münster, Germany
,
Edgar Schafer
4   Central Interdisciplinary Ambulance in the School of Dentistry, Münster, Germany
› Institutsangaben
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Publikationsdatum:
26. September 2019 (online)

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ABSTRACT

Objective: The aim of this study was to evaluate the antimicrobial effect of chlorhexidine gel (CHX-G) 2%, chlorhexidine powder (CHX-P) 1%, povidone-iodine (PVP-I), polyhexanide and camphorated-and-mentholated chlorophenol (ChKM) ex vivo. Materials and Methods: For every medicament group 10 root segments (15 mm long) of extracted human teeth were prepared to ISO-size 45 and sterilized (n = 50). The root segments were then inoculated with Enterococcus faecalis and aerobically incubated at 37°C. After 1 week, ten root canals were filled with one of the medicaments, respectively and aerobically incubated at 37°C for another week. Ten teeth served as positive controls and were filled with sterile saline solution. After 7 days, the medicaments were inactivated and all root canals were instrumented to ISO-size 50. The obtained dentin samples were dispersed in Ringer solution followed by the preparation of serial dilutions. 10 ΅l per sample were applied to an agar plate and incubated at 37°C for 48 h. The colony forming units were counted and the reduction factors (RFs) were calculated and statistically analyzed. Results: Compared with the positive controls all medicaments exhibited an antibacterial effect against E. faecalis. The RFs for CHX-G, CHX-P and ChKM were significantly higher compared to PVP-I and polyhexanide (P < 0.05). In contrast to PVP-I and polyhexanide, CHX-G, CHX-P and ChKM were able to eliminate E. faecalis from all dentin samples. Conclusions: Within the limitations of this ex vivo investigation, 2% CHX-G and CHX-P were as effective as ChKM against E. faecalis. Thus, when choosing a root canal medicament the better biocompatibility of CHX compared with ChKM should be taken in consideration.

Key words:

Camphorated-and-mentholated chlorophenol - chlorhexidine - polyhexanide - povidone-iodine - root canal medication
 

  • REFERENCES

  • 1 Love RM. Enterococcus faecalis: A mechanism for its role in endodontic failure. Int Endod J 2001; 34: 399-405
    CrossrefPubMedGoogle Scholar
  • 2 Rôças IN, Siqueira Jr JF, Aboim MC, Rosado AS. Denaturing gradient gel electrophoresis analysis of bacterial communities associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004; 98: 741-9
    CrossrefPubMedGoogle Scholar
  • 3 Orstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol 1990; 6: 142-9
    CrossrefPubMedGoogle Scholar
  • 4 Figdor D, Davies JK, Sundqvist G. Starvation survival, growth and recovery of Enterococcus faecalis in human serum. Oral Microbiol Immunol 2003; 18: 234-9
    CrossrefPubMedGoogle Scholar
  • 5 Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res 1987; 66: 1375-9
    CrossrefPubMedGoogle Scholar
  • 6 Lenet BJ, Komorowski R, Wu XY, Huang J, Grad H, Lawrence HP. et al. Antimicrobial substantivity of bovine root dentin exposed to different chlorhexidine delivery vehicles. J Endod 2000; 26: 652-5
    CrossrefPubMedGoogle Scholar
  • 7 Basrani B, Tjäderhane L, Santos JM, Pascon E, Grad H, Lawrence HP. et al. Efficacy of chlorhexidine-and calcium hydroxide-containing medicaments against Enterococcus faecalis in vitro . Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003; 96: 618-24
    CrossrefPubMedGoogle Scholar
  • 8 Gomes BP, Souza SF, Ferraz CC, Teixeira FB, Zaia AA, Valdrighi L. et al. Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro . Int Endod J 2003; 36: 267-75
    CrossrefPubMedGoogle Scholar
  • 9 Kanisavaran ZM. Chlorhexidine gluconate in endodontics: An update review. Int Dent J 2008; 58: 247-57
    CrossrefPubMedGoogle Scholar
  • 10 Ansorg RA, Azem T, Fabry WH, Rath PM. Influence of mucin on the activity of the antiseptic lavasept against Staphylococcus aureus . Chemotherapy 2002; 48: 129-33
    CrossrefPubMedGoogle Scholar
  • 11 Ryssel H, Kloeters O, Germann G, Schäfer T, Wiedemann G, Oehlbauer M. The antimicrobial effect of acetic acid - An alternative to common local antiseptics?. Burns 2009; 35: 695-700
    CrossrefPubMedGoogle Scholar
  • 12 Hirsch T, Koerber A, Jacobsen F, Dissemond J, Steinau HU, Gatermann S. et al. Evaluation of toxic side effects of clinically used skin antiseptics in vitro . J Surg Res 2010; 164: 344-50
    CrossrefPubMedGoogle Scholar
  • 13 Koburger T, Hübner NO, Braun M, Siebert J, Kramer A. Standardized comparison of antiseptic efficacy of triclosan, PVP-iodine, octenidine dihydrochloride, polyhexanide and chlorhexidine digluconate. J Antimicrob Chemother 2010; 65: 1712-9
    CrossrefPubMedGoogle Scholar
  • 14 Kaehn K. Polihexanide: A safe and highly effective biocide. Skin Pharmacol Physiol 2010; 23Suppl 1: 7-16
    Google Scholar
  • 15 Hübner NO, Kramer A. Review on the efficacy, safety and clinical applications of polihexanide, a modern wound antiseptic. Skin Pharmacol Physiol 2010; 23Suppl 1: 17-27
    Google Scholar
  • 16 Kramer A, Rudolph P, Werner HP. Antimicrobial efficacy of contact lens care products and critical comment on ISO/FDIS 14729. In: Kramer A, Behrens-Baumann W, editors Antiseptic Prophylaxis and Therapy in Ocular Infections. Basel, Switzerland: Karger; 2002: p. 343-61
    Google Scholar
  • 17 Rosin M, Welk A, Kocher T, Majic-Todt A, Kramer A, Pitten FA. The effect of a polyhexamethylene biguanide mouthrinse compared to an essential oil rinse and a chlorhexidine rinse on bacterial counts and 4-day plaque regrowth. J Clin Periodontol 2002; 29: 392-9
    CrossrefPubMedGoogle Scholar
  • 18 Gottardi W. Iodine and iodine compounds. In: Block SS, editor Disinfection, Sterilization, and Preservation. Philadelphia, USA: Lippincott Williams and Wilkins; 2001. 29. 159-83
    Google Scholar
  • 19 Mohammadi Z. Iodine compounds in endodontics: An update review. Dent Today 2009; 28:58: 60-3
    Google Scholar
  • 20 König B, Reimer K, Fleischer W, König W. Effects of Betaisodona on parameters of host defense. Dermatology 1997; 195Suppl 2: 42-8
    Google Scholar
  • 21 Abdullah M, Ng YL, Gulabivala K, Moles DR, Spratt DA. Susceptibilties of two Enterococcus faecalis phenotypes to root canal medications. J Endod 2005; 31: 30-6
    CrossrefPubMedGoogle Scholar
  • 22 Da Silva GN, De Camargo EA, Salvadori DM, Ribeiro DA. Genetic damage in human peripheral lymphocytes exposed to antimicrobial endodontic agents. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007; 104: 58-61
    Google Scholar
  • 23 Violich DR, Chandler NP. The smear layer in endodontics: A review. Int Endod J 2010; 43: 2-15
    CrossrefPubMedGoogle Scholar
  • 24 Messer HH, Feigal RJ. A comparison of the antibacterial and cytotoxic effects of parachlorophenol. J Dent Res 1985; 64: 818-21
    Google Scholar
  • 25 Koontongkaew S, Silapichit R, Thaweboon B. Clinical and laboratory assessments of camphorated monochlorophenol in endodontic therapy. Oral Surg Oral Med Oral Pathol 1988; 65: 757-62
    CrossrefPubMedGoogle Scholar
  • 26 Versümer J, Hülsmann M. The use of chlorphenol as an endodontic medicament. Endodontie 2003; 12: 165-78
    Google Scholar
  • 27 Schäfer E, Bössmann K. Antimicrobial efficacy of chlorhexidine and two calcium hydroxide formulations against Enterococcus faecalis . J Endod 2005; 31: 53-6
    CrossrefPubMedGoogle Scholar
  • 28 Schäfer E, Bossmann K. Antimicrobial efficacy of chloroxylenol and chlorhexidine in the treatment of infected root canals. Am J Dent 2001; 14: 233-7
    PubMedGoogle Scholar
  • 29 Rödig T, Vogel S, Zapf A, Hülsmann M. Efficacy of different irrigants in the removal of calcium hydroxide from root canals. Int Endod J 2010; 43: 519-27
    CrossrefPubMedGoogle Scholar
  • 30 Fleischer W, Reimer K. Povidone-iodine in antisepsis - State of the art. Dermatology 1997; 195Suppl 2: 3-9
    Google Scholar
  • 31 Haapasalo MP, Endal U, Zandi H, Coil JM. Eradication of endodontic infection by instrumentation and irrigation solutions. Endod Top 2005; 10: 77-102
    CrossrefGoogle Scholar
  • 32 Lin S, Kfir A, Laviv A, Sela G, Fuss Z. The in vitro antibacterial effect of iodine-potassium iodide and calcium hydroxide in infected dentinal tubules at different time intervals. J Contemp Dent Pract 2009; 10: 59-66
    PubMedGoogle Scholar
  • 33 Safavi KE, Spangberg LS, Langeland K. Root canal dentinal tubule disinfection. J Endod 1990; 16: 207-10
    CrossrefPubMedGoogle Scholar
  • 34 Haapasalo HK, Sirén EK, Waltimo TM, Ørstavik D, Haapasalo MP. Inactivation of local root canal medicaments by dentine: An in vitro study. Int Endod J 2000; 33: 126-31
    CrossrefPubMedGoogle Scholar
  • 35 Spångberg L, Rutberg M, Rydinge E. Biologic effects of endodontic antimicrobial agents. J Endod 1979; 5: 166-75
    CrossrefPubMedGoogle Scholar
  • 36 Tepel J, Darwisch el Sawaf M, Hoppe W. Reaction of inflamed periapical tissue to intracanal medicaments and root canal sealers. Endod Dent Traumatol 1994; 10: 233-8
    CrossrefPubMedGoogle Scholar
  • 37 Soekanto A, Kasugai S, Mataki S, Ohya K, Ogura H. Toxicity of camphorated phenol and camphorated parachlorophenol in dental pulp cell culture. J Endod 1996; 22: 284-9
    CrossrefPubMedGoogle Scholar
  • 38 Llamas R, Segura JJ, Jiménez-Rubio A, Jiménez-Planas A. In vitro effect of parachlorophenol and camphorated parachlorophenol on macrophages. J Endod 1997; 23: 728-30
    CrossrefPubMedGoogle Scholar
  • 39 Chang YC, Tai KW, Chou LS, Chou MY. Effects of camphorated parachlorophenol on human periodontal ligament cells in vitro . J Endod 1999; 25: 779-81
    CrossrefPubMedGoogle Scholar
  • 40 Shehata M, Durner J, Thiessen D, Shirin M, Lottner S, Van Landuyt K. et al. Induction of DNA double-strand breaks by monochlorophenol isomers and ChKM in human gingival fibroblasts. Arch Toxicol 2012; 86: 1423-9
    CrossrefPubMedGoogle Scholar