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CC BY-NC-ND 4.0 · Eur J Dent 2013; 07(04): 412-418
DOI: 10.4103/1305-7456.120666
Original Article
Dental Investigation Society

Insights into and relative effect of chitosan-H, chitosan-H-propolis, chitosan-H-propolis-nystatin and chitosan-H-nystatin on dentine bond strength

Victoria Tamara Perchyonok
1   Department of Research and Innovations, VTPCHEM Pty. Ltd., Glenhuntly, Melbourne, 3163, Australia
2   School of Dentistry and Oral Health, Griffith University, Gold Coast, Southport, 4215, QLD, Australia
,
Shengmiao Zhang
3   School of Material Science and Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China
,
Sias R. Grobler
4   Oral and Dental Research Institute, Faculty of Dentistry, University of the Western Cape, Private Bag X1, Tygerberg 7505, Cape Town, South Africa
,
Theunis G. Oberholzer
2   School of Dentistry and Oral Health, Griffith University, Gold Coast, Southport, 4215, QLD, Australia
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Publikationsdatum:
06. November 2019 (online)

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ABSTRACT

Objective: The purpose of the study was to design and evaluate novel functional chitosan hydrogels (chitosan-H-propolis, chitosan-H-propolis-nystatin and chitosan-H-nystatin) by using the chitosan-H polymer as "dual function restorative materials." Materials and Methods: The nystatin/antioxidant carrier gel was prepared by dispersion of the corresponding component in glycerol and 3% acetic acid with 5% chitosan gelling agent was then added to the dispersion with continuous mixing. The natural bio-adhesive functionalized chitosan hydrogels were combined with built in drug delivery system and bio-actives such as propolis in order to increase the dentin bond strength capacity and maintain therapeutic properties of the alternative drug delivery system. The surface morphology, release behaviors (physiological pH and also in acidic conditions), stability of nystatin:antioxidant:chitosan and the effect of the hydrogels on the shear bond strength of dentin were also evaluated.Statistical Analysis Used: Non-parametric ANOVA test was used to asses significance of higher shear bond values than dentine treated or not treated with phosphoric acid. Results: The release of both nystatin and propolis confer the added benefit of dual action of a functional therapeutic delivery when comparing the newly designed chitosan-based hydrogel restorative materials to commercially available nystatin alone. Neither the release of nystatin nor the antioxidant stability was affected by storage. Chitosan-H, chitosan-propolis, chitosan-nystatin and chitosan-nystatin-propolis treated dentine gives significantly (P < 0.05) higher shear bond values (P < 0.05) than dentine treated or not treated with phosphoric acid.Conclusion: The added benefits of their unique functionality involve increased dentin adhesive bond strengths (after 24 h and after 6 months) and positive influence on the nystatin release. Nystatin was a model therapeutic agent, evaluating the concept of using functional materials as carriers for pro-drugs as well as displaying a certain degree of defence mechanism for free radical damage of the novel functional drug delivery. Overall, there was an insignificant relapse in the shear bond strength after 6 months.

Key words:

Antioxidant-chitosan hydrogels - bond strength - functional restorative materials - scanning electron microscopy
 

  • REFERENCES

  • 1 Subramani K, Ahmed W. Emerging Nanotechnologies in Dentistry: Processes, Materials and Applications. 1st ed. Elsevier; 2011
    Google Scholar
  • 2 Hensten-Pettersen A, Helgeland K. Evaluation of biologic effects of dental materials using four different cell culture techniques. Scand J Dent Res 1977; 85: 291-6
    PubMedGoogle Scholar
  • 3 Vasudevan DM, Sreekumari S. Textbook of Biochemistry for Medical Students. 4 th ed. Jaypee; 2004: p. 338-40
    Google Scholar
  • 4 Sharma A, Sharma S. ROS and antioxidants in periodontics: A review. Int J Dent Clin 2011; 3: 44-7
    Google Scholar
  • 5 Diplock AT, Rice-Evans CA, Burdon RH. Is there a significant role for lipid peroxidation in the causation of malignancy and for antioxidants in cancer prevention?. Cancer Res 1994; 54: 1952-6
    Google Scholar
  • 6 Geronikaki AA, Gavalas AM. Antioxidants and inflammatory disease: Synthetic and natural antioxidants with anti-inflammatory activity. Comb Chem High Throughput Screen 2006; 9: 425-42
    CrossrefPubMedGoogle Scholar
  • 7 Subash V, Saritha G, Fareedullha M. Role of antioxidants and oxidative stress in cardiovascular diseases. Ann Biomed Res 2010; 3: 158-73
    Google Scholar
  • 8 Guo RF, Ward PA. Role of oxidants in lung injury during sepsis. Antioxid Redox Signal 2007; 9: 1991-2002
    CrossrefPubMedGoogle Scholar
  • 9 Johansen JS, Harris AK, Rychly DJ, Ergul A. Oxidative stress and the use of antioxidants in diabetes: Linking basic science to clinical practice. Cardiovasc Diabetol 2005; 4: 5
    CrossrefPubMedGoogle Scholar
  • 10 Yadav SB, Suryakar AN, Durgawale PP. Antioxidant treatment a new therapeutic approach to reversible male infertility. Biomed Res 2006; 17: 175-8
    Google Scholar
  • 11 Palmberger TF, Hombach J, Bernkop-Schnürch A. Thiolated chitosan: Development and in vitro evaluation of an oral delivery system for acyclovir. Int J Pharm 2008; 348: 54-60
    CrossrefPubMedGoogle Scholar
  • 12 Macleod GS, Fell JT, Collett JH, Sharma HL, Smith AM. Selective drug delivery to the colon using pectin: Chitosan: Hydroxypropyl methylcellulose film coated tablets. Int J Pharm 1999; 187: 251-7
    CrossrefPubMedGoogle Scholar
  • 13 Bernkop-Schnürch A, Reich-Rohrwig E, Marschütz M, Schuhbauer H, Kratzel M. Development of a sustained release dosage form for alpha-lipoic acid. II. Evaluation in human volunteers. Drug Dev Ind Pharm 2004; 30: 35-42
    CrossrefPubMedGoogle Scholar
  • 14 Kurosaki Y, Kimura T. Regional variation in oral mucosal drug permeability. Crit Rev Ther Drug Carrier Syst 2000; 17: 467-508
    PubMedGoogle Scholar
  • 15 Giamalia I, Steinberg D, Grobler S, Gedalia I. The effect of propolis exposure on microhardness of human enamel in vitro. J Oral Rehabil 1999; 26: 941-3
    CrossrefPubMedGoogle Scholar
  • 16 Banskota AH, Tezuka Y, Prasain JK, Matsushige K, Saiki I, Kadota S. Chemical constituents of Brazilian propolis and their cytotoxic activities. J Nat Prod 1998; 61: 896-900
    CrossrefPubMedGoogle Scholar
  • 17 Foucher D. Thesis. Propolis and its use in Pharmacy. PhD Thesis. Clermont; Ferrand, France: 1982
    Google Scholar
  • 18 Borrelli F, Maffia P, Pinto L, Ianaro A, Russo A, Capasso F. Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia 2002; 73 Suppl 1: 53-63
    CrossrefPubMedGoogle Scholar
  • 19 Gøtzsche PC, Johansen HK. Routine versus selective antifungal administration for control of fungal infections in patients with cancer. Cochrane Database of Systematic Reviews, 2002, Issue 2.
    Google Scholar
  • 20 Buchanan AG, Riben PD, Rayner EN, Parker SE, Ronald AR, Louie TJ. Nystatin prophylaxis of fungal colonization and infection in granulocytopenic patients: Correlation of colonization and clinical outcome. Clin Invest Med 1985; 8: 139-47
    PubMedGoogle Scholar
  • 21 Wood RA. Nystatin-Resistant mutants of yeast: Alterations in sterol content. J Bacteriol 1971; 108: 69-73
    CrossrefPubMedGoogle Scholar
  • 22 Perchyonok VT, Zhang S, Oberholzer T. Alternative chitosan based drug delivery system to fight oral mucositis: Synergy of conventional and bioactives towards the optimal solution. Curr Nanosci 2012; 8: 541-7
    CrossrefGoogle Scholar
  • 23 Akala EO, Kopecková P, Kopecek J. Novel pH-sensitive hydrogels with adjustable swelling kinetics. Biomaterials 1998; 19: 1037-47
    CrossrefPubMedGoogle Scholar
  • 24 Martin A. Physical Pharmacy. Philadelphia, London: Lea and Febiger; 1993 p. 496-7
    Google Scholar
  • 25 Chen S, Liu M, Jin S, Wang B. Preparation of ionic-crosslinked chitosan-based gel beads and effect of reaction conditions on drug release behaviors. Int J Pharm 2008; 349: 180-7
    CrossrefPubMedGoogle Scholar
  • 26 Shin SC, Kim JY. Enhanced permeation of triamcinolone acetonide through the buccal mucosa. Eur J Pharm Biopharm 2000; 50: 217-20
    CrossrefPubMedGoogle Scholar
  • 27 Hamilton-Miller JM. The effect of pH and of temperature on the stability and bioactivity of nystatin and amphotericin B. J Pharm Pharmacol 1973; 25: 401-7
    CrossrefPubMedGoogle Scholar
  • 28 Grobler SR, Majeed A, Moola MH. Effect of various tooth-whitening products on enamel microhardness. SADJ 2009; 64: 474-9
    PubMedGoogle Scholar
  • 29 Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol 1999; 64: 235-40
    CrossrefPubMedGoogle Scholar
  • 30 Zhou J, Xue X, Li Y, Zhang J, Chen F, Wu L. et al. Multi-residue determination of tetracycline antibiotics in propolis by using HPLC-UV detection with ultrasonic-assisted extraction and two-step solid phase extraction. Food Chem 2009; 115: 1074-80
    CrossrefGoogle Scholar
  • 31 Viuda-Martos M, Ruiz-Navajas Y, Fernández-López J, Pérez-Alvarez JA. Functional properties of honey, propolis, and royal jelly. J Food Sci 2008; 73: R117-24
    CrossrefPubMedGoogle Scholar
  • 32 Samšináková A, Kálalová S, Haragsim O. Effects of some antimycotics and disinfectants on the ascosphaera apis maassen fungus in vitro. Z Angew Entomol 1973; 84: 225-32
    Google Scholar
  • 33 Chapple IL. Role of free radicals and antioxidants in the pathogenesis of the inflammatory periodontal diseases. Clin Mol Pathol 1996; 49: M247-55
    CrossrefPubMedGoogle Scholar
  • 34 Allan CR, Hardwiger LA. The fungicidal effect of chitosan on fungi of varying cell wall composition. Exp Mycol 1979; 3: 285-7
    CrossrefGoogle Scholar
  • 35 Nagy I, Floyd RA. Electron spin resonance spectroscopic demonstration of the hydroxyl free radical scavenger properties of dimethylaminoethanol in spin trapping experiments confirming the molecular basis for the biological effects of centrophenoxine. Arch Gerontol Geriatr 1984; 3: 297-310
    CrossrefPubMedGoogle Scholar
  • 36 Zs-Nagy I, Floyd RA. ESR spin trapping studies on the OHFNx01 free radical reactions of idebenone. Arch Gerontol Geriatr 1990; 11: 215-31
    CrossrefPubMedGoogle Scholar
  • 37 Bartold PM, Wiebkin OW, Thonard JC. The effect of oxygen-derived free radicals on gingival proteoglycans and hyaluronic acid. J Periodontal Res 1984; 19: 390-40
    CrossrefPubMedGoogle Scholar
  • 38 Schweikl H, Hartmann A, Hiller KA, Spagnuolo G, Bolay C, Brockhoff G. et al. Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. Dent Mater 2007; 23: 688-95
    CrossrefPubMedGoogle Scholar
  • 39 Godley BF, Shamsi FA, Liang FQ, Jarrett SG, Davies S, Boulton M. Blue light induces mitochondrial DNA damage and free radical production in epithelial cells. J Biol Chem 2005; 280: 21061-6
    CrossrefPubMedGoogle Scholar
  • 40 Perchyonok VT, Zhang S, Oberholzer T. Protective effect of conventional antioxidant (â-carotene, resveratrol and vitamin E) in chitosan-containing hydrogels against oxidative stress and reversal of DNA double stranded breaks induced by common dental composites: In-vitro model. Open Nanosci J 2013; 7: 1-7
    CrossrefGoogle Scholar