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CC BY-NC-ND 4.0 · J Lab Physicians 2010; 2(02): 055-060
DOI: 10.4103/0974-2727.72149
Review Article

Brucellosis: Review on the Recent Trends in Pathogenicity and Laboratory Diagnosis

Supriya Christopher
Department of Microbiology, Kempegowda Institute of Medical Sciences, BSK II Stage, Bangalore, India
,
Umapathy B L.
Department of Microbiology, Kempegowda Institute of Medical Sciences, BSK II Stage, Bangalore, India
,
Ravikumar K L.
Department of Microbiology, Kempegowda Institute of Medical Sciences, BSK II Stage, Bangalore, India
› Institutsangaben Source of Support: Nil
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ABSTRACT

Brucellosis is a zoonotic infection transmitted from animals to humans by the ingestion of infected food products, direct contact with an infected animal or inhalation of aerosols. The last method is remarkably efficient given the relatively low concentration of organisms (10 – 100 bacteria) needed to establish infection in humans, and has brought renewed attention to this old disease. Brucella is a facultative intracellular pathogen that has the ability to survive and multiply in the phagocytes and cause abortion in cattle and undulant fever in humans. Brucella spp particularly B. melitensis, B. abortus, and B. suis represent a significant public health concern. At present, B. melitensis is the principle cause of human brucellosis in India. Molecular studies have demonstrated the phylogenetic affiliation of Brucella to Agrobacterium, Ochrobactrum, and Rhizobium. Human brucellosis still presents scientists and clinicians with several challenges, with regard to the understanding of its pathogenic mechanism, severity, progression, and development of improved treatment regimens. Molecular studies have now highlighted the pathogenesis of Brucella, for the development of newer diagnostic tools that will be useful in developing countries where brucellosis is a common, but often a neglected disease. This review compiles all these issues in general and the pathogenicity and newer diagnostic tools in particular.

Keywords

Brucellosis - lipopolysaccharide - virulence - zoonotic disease - serodiagnosis


Publikationsverlauf

Artikel online veröffentlicht:
29. Januar 2020

© 2010.

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • REFERENCES

  • 1 Cultuer SJ, Whatmore AM, Commander NJ. Brucellosis- new aspects of an old disease. J Appl Microbiol 2005;98:1270-81.
  • 2 Bang B. The etiology of epizootic abortion. J Comp Pathol Therap 1897;10:125.
  • 3 Mantur BG, Amarnath S. Brucellosis in India. J Biosci 2008;33:539-47.
  • 4 Umapathy BL, Nagamani M, Bhat P, Keshavamurthy BS. Seroepidemiological studies on human brucellosis in and around Bangalore. Indian J Comp Microbiol Immunol Infect Dis 1984;5:83-7
  • 5 Godfroid J, Cloekaert A, Liautard JP. From the discovery of the Malta fever’s agent to the discovery ofa marine mammal reservoir, brucellosis has continuously been re-emerging zoonosis. Vet Res 2005;36:313-26.
  • 6 Madkour MM, Madkoure. Brucellosis. New York: Springer Verlag; 2001. p. 11.
  • 7 Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV. The new global map of human brucellosis. Lancet Infect Dis 2006;6:91-9.
  • 8 Colmenero JD, Reguera JM, Cabrera FP. Serology, clinical manifestations and treatment of brucellosis. Infection 1990;18:152-56.
  • 9 Memish Z, Mah MW, Al Mahmoud S, Al Shaalan M, Khan MY. Brucella bacteremia: clinical and laboratory observations in 160 patients. J Infect Dis 2000;40:59-63.
  • 10 Morenoe EE, Stackebrandt E, Dorsch M, Wolters J, Busch M, Mayer II. Brucella. abortus 16Sr RNA and lipid A reveal a phylogenetic relationship with members of the α 2 subdivision of the class proteobacteria. J Bacteriol 1990;172:3569-76.
  • 11 Corbel MJ, Brinley Morgan WJ. Genus Brucella. In: Bergey’s Manual of systematic Bacteriology. Kreig Nr, Holt JG, editors. Baltimore: Williams & Wilkins; 1984. p. 377-88.
  • 12 Cloeckaert A, Grayon M, Grepinet O, Boumedine KS. Classification of Brucella stains isolated from marine mammals by infrequent restriction site –PCR and development of specific PCR identification tests. Microbes Infect 2003;5:593-602.
  • 13 Michaux C, Bang SG, Jumas BE, Guigue T, Allardet A, O’ Callaghan D, et al. Genome structure & phylogeny in the genes Brucella. J Bacteriol 1997;179:3244-49.
  • 14 Harze WD. Brucella virulence. Genome biology; 2002. p. 3.
  • 15 Ko J, Splitter GA. Molecular host interaction in brucellosis: Current understanding and futureapproaches to vaccine development for mice and humans. Clin Microbial Rev 2003;16:65-78.
  • 16 Vidya C, Remi F, Stephane D, Nora C, Jorge N, Gabriel W. Structure of the outer membrane complex of a type IV secretion system. Nature 2009;462:1011-6.
  • 17 DelVecchio VG, Kapatral V, Redkar RJ, Patra G, Mujer C, Los T, et al. The genome sequence of the facultative intracellular pathogen Brucella. melitenisis. Proc Natt Acad Sci USA 2002;99:443-8.
  • 18 Sanchez D, Zandomeni RO, Cravero S, Verdun RE, Pierrou E, Faccio P, et al. Gene discovery through genomic sequencing of Brucella. abortus. Infect Immun 2001;69:865-8.
  • 19 Lapaque N, Moriyon I, Moreno E, Grovel JP. Brucella lipopolysaccharide acts as a virulence factor. Curr Opin Microbiol 2005;8:60-6.
  • 20 Araya IN, Elzer P, Rowge, Enright FM, Winter J. Temporal development of protective cell mediated immunity & humoral immunity in BALB / CMICE infected with Brucella abortus in J 774 Macrophages. Infect Immun 1989;143:3330-7.
  • 21 Bundle DR, Cherwonogrodzky JW, Gidney MAJ, Meckll RJ, Perry MB, Peters T. Definition of Brucella A & M epitopes by monoclonal typing reagents and synthetic oligosaccharides. Infect Immun 1989;57:2829-39.
  • 22 Monero E, Borowiak D, Mayer H. Brucella Lipopolysaccharides and polysaccharides. Ann Inst Pasteur Microbiol 1987;138:102-5.
  • 23 Freer E, Roja SN, Weintraub A, Lindberg AA, Moreno E. Heterogeneity of Brucella. abortus lipopolysaccharides. Res Micrbiol 1995;146:569-78.
  • 24 Raetz CR. Biochemistry of endotoxins. Ann Rev Biochem 1990;59:129-70.
  • 25 Diaz AE, Aragon V, Marin C, Alonso B, Tont M, Moreno E. Comparative analysis of Brucella Serotypes A & M And Yersinia enterocolitica 0:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep and goats. J Clin Microbiol 1993;31:3136-41.
  • 26 Halling SM, Peterson BD, Bricker BJ, Zuernei RL, Quing Z, et al. Completion of the genome sequence of Brucella abortus and comparision to the similar genomes of Brucella. melitensis and Brucella. suis. J Bacteriol 2005;8:2715-26.
  • 27 Dricot A, Rual JF, Lamesch P, Beetm N. Generation of the Brucella melitensis ORFeome version 1. Genome Research 2004;4:2201-06.
  • 28 Patricia G, Cardosa, Gilson Costa CM, Vasco A, Sergi CO. Brucella sps non conocial LPS: Structure, interaction with host immune system. Microbial cell factories 2006;5:131-11.
  • 29 Vizcaino N, Cloeckaert A, Zygmunt MS, Fernandez–Lago L. Characterization of a Brucella species 25-kilobase DNA fragment detected from Brucella abortus reveals a large gene cluster related to the synthesis of a polysaccharide. Infect Immune 2001;69:6738-48.
  • 30 Lopez G, Guzman V, Manterola L. Regulation of Brucella virulence by two component system BvrR / BvrS. Vet Microbiol 2002;90:329-39.
  • 31 Maria PF, Maximillan M, Robert HG, Henk LS. Human brucellosis. Lancet Infect Dis 2007;7:775-86.
  • 32 Arenas GN, Staskevish AS, Aballay A. Intracellular tracffiking of B abortus in J774 macrophages. Infect Immun 2000;68:4255-63.
  • 33 Wataria M. Interaction between Brucella abortus and cellular prion protein in lipid raft microdomains. Microbes Infect 2004;6:93-100.
  • 34 Kittelberger R, Hilbink F, Hansen MF, Ross GP, Joyce MA. Serological cross reactivity between B. abortus and Yersinia enterocolitica 0:9 II the use of Yersinia outer proteins for specific detection of Yersinia enterocolitica infections in ruminants. Vet Microbiol 1997;57:271-80.
  • 35 Ruiz CM. A practical method for routine blood culture in brucellosis. Proc Soc Exp Biol Med 1954;86:154-5.
  • 36 Gotuzz E, Carrillo C, Guersa J, Llosa L. An evaluation of diagnostic methods for brucellosis – the value of bone marrow culture. J Infect Dis 1986;153:122-5.
  • 37 Mantur BG, Biradar MS, Bidri C, Mallana S, Verrappa K, et al. Protean clinical manifestation & diagnostic challenges of human brucellosis in adults: 16 yrs experiancein an endemic area. J Med Microbiol 2006;55:897-903.
  • 38 Ruiz JD, Sanchez G, Rehuera JM, Martin L, Lopez P, Colmenew JD. Rose Bengal test diagnosis of human bucellosis in emergency department in endemic areas. Clin Microbial Infect 2005;11:221-5.
  • 39 Ruiz-Mesa JD, Sanchez–Gonzalez J, Reguera JM, Martin L, Lopez-Palmero S. Diagnostic yield and use for the rapid diagnosis of human brucellosis in emergency departments in endemic areas. Clin Microbiol Infect 2005;11:221-5.
  • 40 Smith HL, Kadri SM. Brucellosis in India: A deceptive infectious disease. Indian J Med Res 2005;122:375-84.
  • 41 Nielsen K, Lin M, Gall D, Jolley M. Flourescence polarization immunoassay: detection of antibody of Brucella abortus. Methods 2000;22:71-6.
  • 42 Lucero NE, Escobar GI, Ayala SM, Silva PN. Fluorescence polarization assay for diagnosis of human brucellosis. J Med Microbial 2003;52:883-7.
  • 43 Hasanjani RMR, Soleimani Amin MJ, Abdoel TH, Sits HL. Application of a rapid and simple Brucella-specific IgM and IgG antibody test for the serodiagnosis of brucellosis in a hospital in Iran. Trans R Soc Trop Med Hyg 2005;9:744-50.
  • 44 Hasan I, Turan B, Omer E, Hayrettin A, Pekcan D, Theresia H, et al. Use of the Brucella IgM and IgG flow assays in the serodiagnosis of human brucellosis in an area endemic for brucellosis. Am J Trop Med Hyg 2004;70:688-94.
  • 45 Casanova A, Ariza J, Rubio M, Masuet C, Díaz R. Brucella capt versus classical tests in serological diagnosis and management of human brucellosis. Clin Vaccine Immunol 2009;16:844-51.
  • 46 Queipo-Ortuño MI, Colmenero JD, Baeza G, Morata P. Comparision between light cycler real time polymerase chain reaction (PCR) assay with serum and PCR, enzyme linked immunosorbant assay with whole blood samples for the diagnosis of human brucellosis. Clin Infect Dis 2005;40:260-4.
  • 47 Boom R, Sol CJ, Salimans MM. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990;28:495-503.
  • 48 Debaeumont C, Falconnet PA, Maurin M. Real time PCR for detection of Brucella Spp DNA in human serum samples. Eur J Clin Microbiol Infect Dis 2005;24:842-5.
  • 49 Nimri LF. Diagnosis of recent and relapsed cases of human brucellosis by PCR assay. BMC Infect Dis 2003;3:5.
  • 50 Keil FW, Khan NJ. Analysis of 506 consecutive positive serologic tests for brucellosis in Saudi Arabia. J Clin Microbiol 1987;25:1384-7.
  • 51 Morta P, Queiyo –ortuno ML, Rodrignej TA, Ordina A. Specificity of a polymerase chain reaction assay of a target sequence on the 31 Kilodalton Brucella antigen DNA used to diagnose human Brucellosis. Eur J Clin Microbiol Infect Dis 2001;20:27-31.
  • 52 Elena N, Maria C, Javier S. Diagnosis of human brucellosis using PCR. Expert Rev Mol Diag 2004;4:115-23.