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CC BY-NC-ND 4.0 · J Lab Physicians 2016; 8(01): 019-024
DOI: 10.4103/0974-2727.176229
Original Article

Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens

Nasar Yousuf Alwahaibi
Department of Allied Health Sciences, Sultan Qaboos University, Muscat, Oman
,
Mariam Said Alsubhi
Department of Pathology, Sultan Qaboos University Hospital, Muscat, Oman
,
Najat Aldairi
Department of Pathology, Sultan Qaboos University Hospital, Muscat, Oman
,
Amna Alshukaili
Department of Pathology, Sultan Qaboos University Hospital, Muscat, Oman
,
Usha Rani Bai
Department of Pathology, Sultan Qaboos University Hospital, Muscat, Oman
› Author Affiliations Financial support and sponsorship: Nil.
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ABSTRACT

Introduction: Most cytology laboratories in all Gulf countries including Oman, use the standard papanicolaou (PAP) method to stain various cytological specimens. The aim of this study was to investigate the possible application of ultrafast PAP (UF‑PAP) method in cytology laboratory.

Materials and Methods: Samples from 46 patients containing 26 body fluids and 20 fine needle aspirations (FNAs) (9 thyroids and 11 breasts) were collected. Two air dried and two wet smears from each sample were prepared and stained by UF‑PAP and the standard PAP stains, respectively. Background, nuclear staining, cell morphology, and overall staining were independently reviewed by two cytoscreeners.

Results: In all cases of FNA, UF-PAP stain gave a good score for the background, nuclear staining, cell morphology, and overall staining when compared with the standard PAP method. Although the correct diagnosis was made in all cases of body cavity fluids cases except in one case, UF-PAP stain gave a fewer score in the assessment of body cavity fluid samples.

Conclusion: The findings of this study support the use of UF‑PAP method in cytology laboratory with a high emphasis on FNA samples.

Key words

Body cavity fluids - cytology - fine needle aspiration - papanicolaou - ultrafast papanicolaou


Publication History

Article published online:
19 April 2020

© 2016.

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  • REFERENCES

  • 1 Gupta S, Chachra KL, Bhadola P, Sodhani P. Modified Papanicolaou staining protocol with minimum alcohol use: A cost-cutting measure for resource-limited settings. Cytopathology 2010;21:229-33.
  • 2 Dighe SB, Ajit D, Pathuthara S, Chinoy R. Papanicolaou stain: Is it economical to switch to rapid, economical, acetic acid, Papanicolaou stain? Acta Cytol 2006;50:643-6.
  • 3 Lemos LB, Baliga M, Cason Z. Ultrafast Papanicolaou stain: One year's experience in a fine needle aspiration service. Acta Cytol 1997;41:1630-1.
  • 4 Miller AB, Chamberlain J, Day NE, Hakama M, Prorok PC. Report on a workshop of the UICC project on evaluation of screening for cancer. Int J Cancer 1990;46:761-9.
  • 5 Yang GC, Alvarez II. Ultrafast Papanicolaou stain. An alternative preparation for fine needle aspiration cytology. Acta Cytol 1995;39:55-60.
  • 6 Sato M, Taniguchi E, Kagiya T, Nunobiki O, Yang Q, Nakamura M, et al. A modified rapid Papanicolaou stain for imprint smears. Acta Cytol 2004;48:461-2.
  • 7 Biswas RR, Paral CC, Dey R, Biswas SC. Rapid economic, acetic acid, Papanicolaou stain (REAP) – Is it suitable alternative to standard PAP stain? Al Ameen J Med Sci 2008;1:99-103.
  • 8 Choudhary P, Sudhamani S, Pandit A, Kiri V. Comparison of modified ultrafast Papanicolaou stain with the standard rapid Papanicolaou stain in cytology of various organs. J Cytol 2012;29:241-5.
  • 9 Shinde PB, Pandit AA. Application of modified Ultrafast Papanicolaou stain in cytology of various organs. Diagn Cytopathol 2006;34:135-9.
  • 10 Kamal MM, Kulkarni MM, Wahane RN. Ultrafast Papanicolaou stain modified for developing countries: Efficacy and pitfalls. Acta Cytol 2011;55:205-12.
  • 11 Yang GC, Papellas J, Wu HC, Waisman J. Application of Ultrafast Papanicolaou stain to body fluid cytology. Acta Cytol 2001;45:180-5.
  • 12 Maruta J, Hashimoto H, Yamashita H, Yamashita H, Noguchi S. Quick aspiration cytology for thyroid nodules by modified Ultrafast Papanicolaou staining. Diagn Cytopathol 2003;28:45-8.
  • 13 Yang GC. Clear nuclei are specific to papillary carcinoma in thyroid fine-needle aspirates processed by Ultrafast Papanicolaou stain. Diagn Cytopathol 2003;29:236-7.
  • 14 Yang GC, Liebeskind D, Messina AV. Ultrasound-guided fine-needle aspiration of the thyroid assessed by Ultrafast Papanicolaou stain: Data from 1135 biopsies with a two- to six-year follow-up. Thyroid 2001;11:581-9.
  • 15 Bandlish U, Kapoor K, Shukla S. Efficacy of a modified Ultrafast Papanicolaou (UFP) stain for breast aspirates. J Indian Med Assoc 2004;102:309, 312, 326.
  • 16 Yang GC, Liebeskind D, Messina AV. Diagnostic accuracy of follicular variant of papillary thyroid carcinoma in fine-needle aspirates processed by Ultrafast Papanicolaou stain: Histologic follow-up of 125 cases. Cancer 2006;108:174-9.
  • 17 Einstein AJ, Yang GC, Silberfarb JB, Gil J. Effect of Ultrafast Papanicolaou staining on nuclear and textural features in breast cancer cytology. Anal Quant Cytol Histol 1997;19:361-7.
  • 18 Kamal MM, Bodele A, Munshi MM, Bobhate SK, Kher AV. Efficacy of a modified Ultra Fast Papanicolaou (UFP) stain for breast aspirates. Indian J Pathol Microbiol 2000;43:417-21.
  • 19 Bando K, Haba R, Kushida Y, Kadota K, Katsuki N, Ohmichi K, et al. Utility of immediate cytologic diagnosis of lung masses using Ultrafast Papanicolaou stain. Lung Cancer 2011;72:172-6.