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DOI: 10.4103/0971-5851.138994
Is there any role of mast cell density and microvessel density in cervical squamous cell carcinoma? A histologic study with special reference to CD-34 immunomarker staining
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Abstract
Background: Mast cells are involved in induction of angiogenesis in the early-stages of tumor development and in modulating blood vessel growth in the later stages of tumor progression. Aims and Objectives: This study was carried out to evaluate the association between mast cell density (MCD) and microvessel density (MVD) in carcinoma in situ (CIS), microinvasive carcinoma (CA) and invasive squamous cell CA of cervix. Materials and Methods: Six cases of CIS, four cases of microinvasive CA and 38 cases of invasive CA were studied over a period of 2 years from August, 2011 to June, 2013. Ten control samples were included in the study. Routine histologic examination was done. Toluidine blue stain was used for MCD determination. Immunohistochemical analysis with CD-34 was done for assessing MVD. Student′s t-test was used to calculate the statistical significance of MCD and MVD. Results: Both MCD and MVD increased from normal samples through CIS to invasive cervical CA. In the four cases of microinvasive CA, the MCD and MVD were more than that of the control samples, but less than that of the six cases of CIS. Conclusion: There is a correlation between mast cell accumulation and angiogenesis in CIS, microinvasive CA and invasive cervical squamous cell CA. MCD and MVD in invasive CA exceed those in CIS and microinvasive CA. It gives us an opportunity to postulate that therapeutic strategies against mast cell mediators and angiogenesis may be of benefit in patients of early-stage cervical CA.
Publikationsverlauf
Artikel online veröffentlicht:
19. Juli 2021
© 2014. Indian Society of Medical and Paediatric Oncology. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/.)
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References
-
1 Rodewald HR, Feyerabend TB. Widespread immunological functions of mast cells: Fact or fiction? Immunity 2012;37:13-24.
-
2 de Souza DA Jr, Toso VD, Campos MR, Lara VS, Oliver C, Jamur MC. Expression of mast cell proteases correlates with mast cell maturation and angiogenesis during tumor progression. PLoS One 2012;7:e40790.
-
3 Folkman J, Merler E, Abernathy C, Williams G. Isolation of a tumor factor responsible for angiogenesis. J Exp Med 1971;133:275-88.
-
4 Kessler DA, Langer RS, Pless NA, Folkman J. Mast cells and tumor angiogenesis. Int J Cancer 1976;18:703-9.
-
5 Starkey JR, Crowle PK, Taubenberger S. Mast-cell-deficient W/Wv mice exhibit a decreased rate of tumor angiogenesis. Int J Cancer 1988;42:48-52.
-
6 Acikalin MF, Oner U, Topçu I, Yasar B, Kiper H, Colak E. Tumour angiogenesis and mast cell density in the prognostic assessment of colorectal carcinomas. Dig Liver Dis 2005;37:162-9.
-
7 Bochner BH, Cote RJ, Weidner N, Groshen S, Chen SC, Skinner DG, et al. Angiogenesis in bladder cancer: Relationship between microvessel density and tumor prognosis. J Natl Cancer Inst 1995;87:1603-12.
-
8 Erovic BM, Neuchrist C, Berger U, El-Rabadi K, Burian M. Quantitation of microvessel density in squamous cell carcinoma of the head and neck by computer-aided image analysis. Wien Klin Wochenschr 2005;117:53-7.
-
9 Rosai J, Ackerman LV. Uterus, cervix. In: Rosai J, Ackerman LV, editors. Rosai and Ackerman′s Surgical Pathology. 10 th ed. New Delhi: Elsevier; 2011. p. 1436-76.
-
10 Ehrlich P. Contributions to the theory and practice of histological staining (Inaugural dissertation, University of Leipzig, 1878). In: Himmelweit F, editor. Collected Papers of Paul Ehrlich. Vol. 1. London: Pergamon Press; 1956. p. 65-98.
-
11 Galinsky DS, Nechushtan H. Mast cells and cancer - no longer just basic science. Crit Rev Oncol Hematol 2008;68:115-30.
-
12 Maltby S, Khazaie K, McNagny KM. Mast cells in tumor growth: Angiogenesis, tissue remodelling and immune-modulation. Biochim Biophys Acta 2009;1796:19-26.
-
13 Moon TC, St Laurent CD, Morris KE, Marcet C, Yoshimura T, Sekar Y, et al. Advances in mast cell biology: New understanding of heterogeneity and function. Mucosal Immunol 2010;3:111-28.
-
14 Theoharides TC, Conti P. Mast cells: The Jekyll and Hyde of tumor growth. Trends Immunol 2004;25:235-41.
-
15 Kalesnikoff J, Galli SJ. New developments in mast cell biology. Nat Immunol 2008;9:1215-23.
-
16 Gilfillan AM, Beaven MA. Regulation of mast cell responses in health and disease. Crit Rev Immunol 2011;31:475-529.
-
17 Wilk M, Liszka L, Palen P, Gabriel A, Laudanski P. Intensity of angiogenesis and mast cell infiltration in cervical intraepithelial and invasive lesions - are they correlated? Pathol Res Pract 2010;206:217-22.
-
18 Cabanillas-Saez A, Schalper JA, Nicovani SM, Rudolph MI. Characterization of mast cells according to their content of tryptase and chymase in normal and neoplastic human uterine cervix. Int J Gynecol Cancer 2002;12:92-8.
-
19 Utrera-Barillas D, Castro-Manrreza M, Castellanos E, Gutiérrez-Rodríguez M, Arciniega-Ruíz de Esparza O, García-Cebada J, et al. The role of macrophages and mast cells in lymphangiogenesis and angiogenesis in cervical carcinogenesis. Exp Mol Pathol 2010;89:190-6.
-
20 Benítez-Bribiesca L, Wong A, Utrera D, Castellanos E. The role of mast cell tryptase in neoangiogenesis of premalignant and malignant lesions of the uterine cervix. J Histochem Cytochem 2001;49:1061-2.
-
21 Sotiropoulou M, Diakomanolis E, Elsheikh A, Loutradis D, Markaki S, Michalas S. Angiogenic properties of carcinoma in situ and microinvasive carcinoma of the uterine cervix. Eur J Gynaecol Oncol 2004;25:219-21.
-
22 Naik R, Pai MR, Poornima Baliga B, Nayak KS, Shankarnarayana, Dighe P. Mast cell profile in uterine cervix. Indian J Pathol Microbiol 2004;47:178-80.
-
23 Jain PC, Singh SN, Pratap VK, Lahiri B. Connective tissue changes and mast cell variations in benign and malignant lesions of the uterine cervix. Int Surg 1977;62:358-60.
-
24 Vieira SC, Zeferino LC, Da Silva BB, Aparecida Pinto G, Vassallo J, Carasan GA, et al. Quantification of angiogenesis in cervical cancer: A comparison among three endothelial cell markers. Gynecol Oncol 2004;93:121-4.
-
25 Randall LM, Monk BJ, Darcy KM, Tian C, Burger RA, Liao SY, et al. Markers of angiogenesis in high-risk, early-stage cervical cancer: A Gynecologic Oncology Group study. Gynecol Oncol 2009;112:583-9.
-
26 Ancuta C, Ancuta E, Zugun-Eloae F, Carasevici E. Neoangiogenesis in cervical cancer: Focus on CD34 assessment. Rom J Morphol Embryol 2010;51:289-94.
-
27 Lenczewski A, Terlikowski SJ, Sulkowska M, Famulski W, Sulkowski S, Kulikowski M. Prognostic significance of CD34 expression in early cervical squamous cell carcinoma. Folia Histochem Cytobiol 2002;40:205-6.
-
28 Tjalma W, Van Marck E, Weyler J, Dirix L, Van Daele A, Goovaerts G, et al. Quantification and prognostic relevance of angiogenic parameters in invasive cervical cancer. Br J Cancer 1998;78:170-4.
-
29 Mukherjee S, Bandyopadhyay G, Dutta C, Bhattacharya A, Karmakar R, Barui G. Evaluation of endoscopic biopsy in gastric lesions with a special reference to the significance of mast cell density. Indian J Pathol Microbiol 2009;52:20-4.
-
30 Ribatti D, Guidolin D, Marzullo A, Nico B, Annese T, Benagiano V, et al. Mast cells and angiogenesis in gastric carcinoma. Int J Exp Pathol 2010;91:350-6.
-
31 Elezoglu B, Tolunay S. The relationship between the stromal mast cell number, microvessel density, c-erbB-2 staining and survival and prognostic factors in colorectal carcinoma. Turk Patoloji Derg 2012;28:110-8.