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DOI: 10.1590/S1809-48722011000200014
Organic Bovine Graft Associated With PRP In Rabbit Calvaria
Enxerto Bovino Orgânico Associado ao PRP em Calvária de CoelhosPublikationsverlauf
18. November 2010
09. März 2011
Publikationsdatum:
12. Februar 2014 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00026220/201102/lookinside/thumbnails/10-1590-s-1809-48722011000200014_150214-1.jpg)
Summary
Introduction: Repairing large bone defects is a huge challenge that reconstructive surgery currently faces.
Objective: The objective of this study was to perform the histological evaluation of bone repair in rabbit calvaria when using bovine bone graft (Gen-ox-organic®) associated with platelet-rich plasma (PRP).
Method: 12 rabbits were used and two bone fragments were bilaterally removed from calvaria. Then, 24 surgical sites were randomly divided into 3 groups: coagulum (group I), organic (group II) and PRP-included organic (group III). After four weeks, the animals were sacrificed and the grafted area removed, fixed in 10% formalin with PBS 0.1 M, and embedded in paraffin.
Study method: The analyzed histological parameters were: defective area filled with the newly-formed bone, graft's giant cells and particles, as well as the new bone formation associated with the particles. Group I's defects were filled with fibrous tissue attaching the periosteum and revealed a little bone formation peripherally. In both groups II and III, a similar standard was noticed in addition to the absence of graft particles and giant cells. There was no significant difference in the number of giant cells, graft particles and new bone formation around the particles between the grafted material and the PRP-related group.
Conclusion: The results achieved indicate that the organic biomaterial neither separately nor jointly with PRP improves bone regeneration.
Resumo
Introdução: O reparo ósseo de grandes defeitos é um grande desafio para a cirurgia reconstrutora atualmente.
Objetivo: O objetivo desse estudo foi realizar avaliação histológica do reparo ósseo em calvária de coelhos depois do uso de enxerto ósseo bovino (Gen-ox-organic®) associado a plasma rico em plaquetas (PRP).
Método: Foram utilizados 12 coelhos, e dois fragmentos ósseos foram removidos da calvária bilateralmente. Então 24 sítios cirúrgicos foram aleatoriamente separados em 3 grupos: coágulo (grupo I), orgânico (grupo II) e orgânico com PRP (grupo III). Depois de quatro semanas, os animais foram sacrificados e a área enxertada foi removida, fixada em formol a 10%, em PBS 0,1M e incluídas em parafina.
Resultados: Os parâmetros histológicos analisados foram: área do defeito preenchida com osso neoformado, presença de células gigantes e partículas do enxerto, e neoformação óssea associada com as partículas. Os defeitos do grupo I foram preenchidos com tecido fibroso que condicionou o periósteo e apresentou uma pequena formação óssea na periferia. Nos grupos II e III, um padrão semelhante foi observado e também ausência de partículas do enxerto e células gigantes. Não houve diferença significativa no número de células gigantes, partículas do enxerto e neoformação óssea em volta das partículas entre o material enxertado e o grupo com PRP associado.
Conclusão: Os resultados obtidos indicam que o biomaterial orgânico isolado ou em associação com o PRP não melhoraram a regeneração óssea.
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