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CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2019; 77(02): 106-114
DOI: 10.1590/0004-282X20180147
Article

The anticonvulsant effects of Ducrosia anethifolia (Boiss) essential oil are produced by its main component alpha-pinene in rats

Os efeitos anticonvulsivantes do óleo essencial de Ducrosia anethifolia (Boiss) são realizados pelo seu principal componente alfa-pineno em ratos
Mahnaz Zamyad
1   Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, Kerman, Iran
,
Mehdi Abbasnejad 
1   Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, Kerman, Iran
,
Saeed Esmaeili-Mahani
1   Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, Kerman, Iran
,
Ali Mostafavi
2   Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Chemistry, Kerman, Iran
,
Vahid Sheibani
3   Kerman University of Medical Sciences, Kerman Neuroscience Research Center (KNRC), Laboratory of Molecular Neuroscience, Kerman, Iran
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ABSTRACT

Ducrosia anethifolia has been recommended as a remedy for neurological disorders. However, the anticonvulsant effects of D. anethifolia essential oil (DAEO) and its major constituent α-pinene have not yet been clarified.

Methods: A rat model of pentylenetetrazole (PTZ)-induced convulsions was used. Oxidant and antioxidant parameters were assayed in the temporal lobe.

Results: The data showed that DAEO (50, 100 and 200 mg/kg, i.p.) and α-pinene (0.2 and 0.4 mg/kg i.p.) delayed the initiation time, and reduced the duration of myoclonic and tonic-clonic seizures following PTZ injection. The PTZ produced oxidative stress so that malondialdehyde and hydrogen peroxide levels were increased and catalase and peroxidase activity decreased. Pretreatment with DAEO and α-pinene significantly inhibited the above-mentioned enzymatic changes in PTZ-treated animals.

Conclusion: The results suggest that α-pinene, at teast in part, was responsible for the induction of the anticonvulsant and antioxidant effects of DAEO in rats.

RESUMO

A Ducrosia anethifolia tem sido recomendada como remédio para os distúrbios neurológicos. No entanto, os efeitos anticonvulsivantes do óleo essencial de Ducrosia anethifolia (DAEO) e do seu principal constituinte atfa-pineno (α-pineno) ainda não foram clarificados.

Métodos: Foi utilizado um modelo de rato de convulsões induzidas por pentilenotetrazol (PTZ). Os parâmetros oxidante e antioxidante foram ensaiados no lobo temporal do cérebro.

Resultados: Os dados mostraram que DAEO (50, 100 e 200 mg / kg, i.p.) e α-pineno (0,2 e 0,4 mg / kg i.p.) retardaram o tempo de iniciação e reduziram a duração das crises mioclçnicas e tçnico-clçnicas após a injeção de PTZ. O PTZ produziu estresse oxidativo, de modo que os níveis de malondialdeído (MDA) e de peróxido de hidrogênio aumentaram e a atividade da catalase e da peroxidase diminuiu. O pré-tratamento com DAEO e α-pineno inibiu significativamente as alterações enzimáticas mencionadas em animais tratados com PTZ.

Conclusão: O resultado sugere que α-pineno, peto menos em parte, é responsável peta indução dos efeitos anticonvulsivantes e antioxidantes da DAEO em ratos.

Keywords:

Pentytenetetrazote - seizures - oxidative stress

Palavras-chave:

Ducrosia anethifolia - α-pinene - Pentilenotetrazol - Crise - Estresse oxidativo

Support

Shahid Bahonar University and Kerman Neuroscience Research Center (KNRC/94).




Publikationsverlauf

Eingereicht: 29. November 2017

Angenommen: 16. Oktober 2018

Artikel online veröffentlicht:
21. August 2023

© 2023. Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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