Download PDF
CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2022; 80(05): 469-474
DOI: 10.1590/0004-282X-ANP-2021-0002
Articles

Study on the serum level of CoQ10B in patients with Moyamoya disease and its mechanism of affecting disease progression

Estudo sobre o nível sérico de CoQ10B em pacientes com doença de Moyamoya e seu mecanismo de afetar a progressão da doença
Jian MA 
1   Zhengzhou University, The Fifth Affiliated Hospital of Zhengzhou, Department of Neurosurgery, Henan, China.
,
Xudong FU 
1   Zhengzhou University, The Fifth Affiliated Hospital of Zhengzhou, Department of Neurosurgery, Henan, China.
,
Shaolong ZHOU 
1   Zhengzhou University, The Fifth Affiliated Hospital of Zhengzhou, Department of Neurosurgery, Henan, China.
,
Enping MENG 
1   Zhengzhou University, The Fifth Affiliated Hospital of Zhengzhou, Department of Neurosurgery, Henan, China.
,
Zhuo YANG 
1   Zhengzhou University, The Fifth Affiliated Hospital of Zhengzhou, Department of Neurosurgery, Henan, China.
,
Hengwei ZHANG 
1   Zhengzhou University, The Fifth Affiliated Hospital of Zhengzhou, Department of Neurosurgery, Henan, China.
› Author AffiliationsSupport: The present study was supported by the Joint Co-construction Project of Henan Medical Science and Technology Research Plan, [LHGJ20190416].
› Further Information
Also available at
   Permissions and Reprints

ABSTRACT

Background: At present, the etiology and pathogenesis of Moyamoya disease (MMD) are not completely clear. Patients are usually diagnosed after cerebrovascular events. Therefore, it is of great clinical significance to explore the predictive factors of MMD. Objective: This study aimed to investigate the serum level of CoQ10B, the amount of endothelial progenitor cells (EPCs), and mitochondrial function of EPCs in MMD patients. Methods: Forty-one MMD patients and 20 healthy controls were recruited in this study. Patients with MMD were divided into two groups: Ischemic type (n=23) and hemorrhagic type (n=18). Blood samples were collected from the antecubital vein and analyzed by CoQ10B ELISA and flow cytometry. Measures of mitochondrial function of EPCs include oxygen consumption rate (OCR), mitochondrial membrane potential, Ca2+ concentration, adenosine triphosphatases activity and ROS level. Results: The serum CoQ10B level in MMD patients was significantly lower than that in healthy controls (p<0.001). The relative number of EPCs in MMD patients was significantly higher than that in healthy controls (p<0.001). Moreover, the OCR, mitochondrial membrane potential and ATPase activity were decreased and the Ca2+ and reactive oxygen species levels were increased in MMD patients (p<0.001). Conclusions: Our results showed obviously decreased serum CoQ10B level and increased EPCs number in patients with MMD compared with healthy patients, and the mitochondria function of EPCs in MMD patients was abnormal.

RESUMO

Antecedentes: No momento, a etiologia e a patogênese da doença de Moyamoya (DMM) não são completamente claras. Os pacientes geralmente são diagnosticados após eventos cerebrovasculares. Sendo assim, é de grande importância clínica explorar os fatores preditivos de DMM. Objetivo: Este estudo teve como objetivo investigar o nível sérico de CoQ10B, a quantidade de células progenitoras endoteliais (CPE) e a função mitocondrial de CPE em pacientes com DMM. Métodos: Quarenta e um pacientes com DMM e 20 controles saudáveis foram recrutados neste estudo. Aqueles com DMM foram divididos em dois grupos: tipo isquêmico (n=23) e tipo hemorrágico (n=18). Amostras de sangue foram coletadas da veia antecubital e analisadas por CoQ10B Ensaio de Imunoadsorção Enzimática (ELISA) e citometria de fluxo. As medidas da função mitocondrial de CPE incluem taxa de consumo de oxigênio (TCO), potencial de membrana mitocondrial, concentração de Ca2+, atividade de adenosina trifosfatases (ATPase) e nível de espécies reativas de oxigênio (ROS). Resultados: O nível sérico de CoQ10B em pacientes com DMM foi significativamente menor do que em controles saudáveis (p<0,001). O número relativo de CPE em pacientes com MMD foi significativamente maior do que em controles saudáveis (p<0,001). Além disso, a TCO, o potencial de membrana mitocondrial e a atividade ATPase diminuíram e os níveis de Ca2+e ROS aumentaram em pacientes com MMD (p<0,001). Conclusões: Nossos resultados mostraram obviamente diminuição do nível sérico de CoQ10B e aumento do número de CPE em pacientes com DMM em comparação com pacientes saudáveis, e a função mitocondrial de CPE em pacientes com DMM estava anormal.

Keywords:

Moyamoya Disease - Endothelial Progenitor Cells - Mitochondria

Palavras-chave:

Doença de Moyamoya - Células Progenitoras Endoteliais - Mitocôndrias

Authors’ contributions:

JM: conducted most of the experiments and wrote the manuscript; SLZ, EPM, ZY, HWZ: conducted the experiments and analyzed the data; XDF designed the study and revised the manuscript. All authors have read and approved the manuscript.


Support

The present study was supported by the Joint Co-construction Project of Henan Medical Science and Technology Research Plan, [LHGJ20190416].




Publication History

Received: 29 March 2021

Accepted: 21 July 2021

Article published online:
06 February 2023

© 2022. Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Thieme Revinter Publicações Ltda.
Rua do Matoso 170, Rio de Janeiro, RJ, CEP 20270-135, Brazil