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DOI: 10.1160/th15-06-0462
Zinc promotes clot stability by accelerating clot formation and modifying fibrin structure
Financial support: This work was supported in part by the Canadian Institutes of Health Research and the Heart and Stroke Foundation. J.I.W. holds the Heart and Stroke Foundation J. Fraser Mustard Endowed Chair in Cardiovascular Research and the Canada Research Chair (Tier 1) in Thrombosis. The work by J. X., H. W., and D. A. W. was supported in part by the Harvard MRSEC (DMR 14–20570).Publication History
Received:
08 June 2015
Accepted after major revision:
14 September 2015
Publication Date:
20 March 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/201603/lookinside/thumbnails/10-1160-th15-06-0462-1.jpg)
Summary
Zinc released from activated platelets binds fibrin(ogen) and attenuates fibrinolysis. Although zinc also affects clot formation, the mechanism and consequences are poorly understood. To address these gaps, the effect of zinc on clot formation and structure was examined in the absence or presence of factor (F) XIII. Zinc accelerated a) plasma clotting by 1.4-fold, b) fibrinogen clotting by 3.5- and 2.3-fold in the absence or presence of FXIII, respectively, c) fragment X clotting by 1.3-fold, and d) polymerisation of fibrin monomers generated with thrombin or batroxobin by 2.5– and 1.8-fold, respectively. Whereas absorbance increased up to 3.3-fold when fibrinogen was clotted in the presence of zinc, absorbance of fragment X clots was unaffected by zinc, consistent with reports that zinc binds to the αC-domain of fibrin(ogen). Scanning electron microscopic analysis revealed a twofold increase in fibre diameter in the presence of zinc and in permeability studies, zinc increased clot porosity by 30-fold with or without FXIII. Whereas FXIII increased clot stiffness from 128 ± 19 Pa to 415 ± 27 Pa in rheological analyses, zinc reduced clot stiffness by 10– and 8.5-fold in the absence and presence of FXIII, respectively. Clots formed in the presence of zinc were more stable and resisted rupture with or without FXIII. Therefore, zinc accelerates clotting and reduces fibrin clot stiffness in a FXIII-independent manner, suggesting that zinc may work in concert with FXIII to modulate clot strength and stability.
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