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Thromb Haemost 2013; 110(06): 1207-1214
DOI: 10.1160/TH13-07-0623
Platelets and Blood Cells
Schattauer GmbH

MiR-223 is dispensable for platelet production and function in mice

Simon Leierseder
1   Institut für Pharmakologie und Toxikologie, Technische Universität München, Munich, Germany
,
Tobias Petzold   *
2   Medizinische Klinik und Poliklinik I, Klinikum der Universität, Ludwig-Maximilians-Universität, Munich, Germany
,
Lin Zhang   *
2   Medizinische Klinik und Poliklinik I, Klinikum der Universität, Ludwig-Maximilians-Universität, Munich, Germany
3   Current affiliations: Heart Failure Institute, Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China
4   Current affiliation: Department of Cardiovascular Medicine, East Hospital, Tongji University School of Medicine, Shanghai, China
,
Xavier Loyer
1   Institut für Pharmakologie und Toxikologie, Technische Universität München, Munich, Germany
5   Current affiliation: Inserm U970, Paris Cardiovascular Research Center, Paris, France
,
Steffen Massberg
2   Medizinische Klinik und Poliklinik I, Klinikum der Universität, Ludwig-Maximilians-Universität, Munich, Germany
6   DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany
,
Stefan Engelhardt
1   Institut für Pharmakologie und Toxikologie, Technische Universität München, Munich, Germany
6   DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany
› Author Affiliations Financial support: This work was supported in part by grants from the Fondation Leducq and the Bavarian Ministry of Sciences, Research and the Arts in the framework of the Bavarian Molecular Biosystems Research Network (to S.E.) and the DFG (SFB914) and the FP7 EU project PRESTIGE (to S.M.).
Further Information

Publication History

Received: 29 July 2013

Accepted after major revision: 14 September 2013

Publication Date:
30 November 2017 (online)

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Referred to by:

miR-223: sailing to terra incognita for microRNAs in plateletsThromb Haemost 2013; 110(12): 1112-1113
DOI: 10.1160/TH13-10-0882

Summary

MicroRNAs (miRNAs) are key physiological regulators in multiple cell types. Here, we assessed platelet production and function in mice deficient in miR-223, one of the most abundantly expressed miRNAs in platelets and megakaryocytes. We found platelet number, size, lifespan as well as surface expression of platelet adhesion receptors to be unchanged in miR-223-deficient mice. Likewise, loss of miR-223 did not affect platelet activation, adhesion and aggregation and also had no effect on bleeding times. Moreover, miR-223 null megakaryocytes developed normally and were capable to form pro-platelets. However, we detected a transient delay in the recovery of platelet numbers following antibody-induced platelet depletion in miR-223-deficient animals. This delay was not observed after transplantation of bone marrow from miR-223-deficient animals into wild-type recipients, indicating a non-cell-autonomous role of miR-223 for thrombopoiesis. Overall, our data indicate a surprisingly modest role of miR-223 in platelet production, while the function of platelets does not seem to depend on miR-223.

Keywords

MicroRNA - platelets - megakaryocytes - transgenic mice

* These authors contributed equally.


 

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