Download PDF
Thromb Haemost 2009; 102(04): 792-794
DOI: 10.1160/TH09-06-0379
Letters to the Editor
Schattauer GmbH

Normal filopodia extension in VASP-deficient platelets upon activation by adhesive matrices or soluble agonists

Sandra Eliautou
1   UMR_S 949 INSERM-Université de Strasbourg, Etablissement Français du Sang-Alsace, Strasbourg Cedex, France
,
Pierre Henri Mangin
1   UMR_S 949 INSERM-Université de Strasbourg, Etablissement Français du Sang-Alsace, Strasbourg Cedex, France
,
Ulrich Walter
2   Institute of Clinical Biochemistry and Pathobiochemistry, University of Würzburg, Würzburg, Germany
,
Christian Gachet
1   UMR_S 949 INSERM-Université de Strasbourg, Etablissement Français du Sang-Alsace, Strasbourg Cedex, France
,
François Lanza
1   UMR_S 949 INSERM-Université de Strasbourg, Etablissement Français du Sang-Alsace, Strasbourg Cedex, France
› Author Affiliations
Further Information

Publication History

Received: 19 June 2008

Accepted: 07 July 2009

Publication Date:
24 November 2017 (online)

  PDF Download  Permissions and Reprints
 

  • References

  • 1 Allen RD, Zacharski LR, Widirstky ST. et al. Transformation and motility of human platelets: details of the shape change and release reaction observed by optical and electron microscopy. J Cell Biol 1979; 83: 126-142.
    CrossrefPubMedGoogle Scholar
  • 2 Yuan Y, Kulkarni S, Ulsemer P. et al. The von Willebrand factor-glycoprotein Ib/V/IX interaction induces actin polymerization and cytoskeletal reorganization in rolling platelets and glycoprotein Ib/V/IX-transfected cells. J Biol Chem 1999; 274: 36241-36251.
    CrossrefPubMedGoogle Scholar
  • 3 Savage B, Ruggeri ZM. Selective recognition of adhesive sites in surface-bound fibrinogen by glycoprotein IIb-IIIa on nonactivated platelets. J Biol Chem 1991; 266: 11227-11233.
    PubMedGoogle Scholar
  • 4 Hensler ME, Frojmovic M, Taylor RG. et al. Platelet morphologic changes and fibrinogen receptor localization. Initial responses in ADP-activated human platelets. Am J Pathol 1992; 141: 707-719.
    PubMedGoogle Scholar
  • 5 Maxwell MJ, Dopheide SM, Turner SJ. et al. Shear induces a unique series of morphological changes in translocating platelets: effects of morphology on translocation dynamics. Arterioscler Thromb Vasc Biol 2006; 26: 663-669.
    PubMedGoogle Scholar
  • 6 Faix J, Rottner K. The making of filopodia. Curr Opin Cell Biol 2006; 18: 18-25.
    CrossrefPubMedGoogle Scholar
  • 7 Chhabra ES, Higgs HN. The many faces of actin: matching assembly factors with cellular structures. Nat Cell Biol 2007; 09: 1110-1121.
    CrossrefPubMedGoogle Scholar
  • 8 Schirenbeck A, Arasada R, Bretschneider T. et al. The bundling activity of vasodilator-stimulated phosphoprotein is required for filopodium formation. Proceedings of the National Academy of Sciences of the United States of America 2006; 103: 7694-7699.
    CrossrefPubMedGoogle Scholar
  • 9 Kwiatkowski AV, Gertler FB, Loureiro JJ. Function and regulation of Ena/VASP proteins. Trends Cell Biol 2003; 13: 386-392.
    CrossrefPubMedGoogle Scholar
  • 10 Krause M, Dent EW, Bear JE. et al. Ena/VASP proteins: regulators of the actin cytoskeleton and cell migration. Annu Rev Cell Dev Biol 2003; 19: 541-564.
    CrossrefPubMedGoogle Scholar
  • 11 Reinhard M, Jarchau T, Walter U. Actin-based motility: stop and go with Ena/VASP proteins. Trends Biochem Sci 2001; 26: 243-249.
    CrossrefPubMedGoogle Scholar
  • 12 Barzik M, Kotova TI, Higgs HN. et al. Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins. J Biol Chem 2005; 280: 28653-28662.
    CrossrefPubMedGoogle Scholar
  • 13 Harbeck B, Huttelmaier S, Schluter K. et al. Phosphorylation of the vasodilator-stimulated phosphoprotein regulates its interaction with actin. J Biol Chem 2000; 275: 30817-30825.
    CrossrefPubMedGoogle Scholar
  • 14 Schwarz UR, Geiger J, Walter U. et al. Flow cytometry analysis of intracellular VASP phosphorylation for the assessment of activating and inhibitory signal transduction pathways in human platelets – definition and detection of ticlopidine/clopidogrel effects. Thrombosis and haemostasis 1999; 82: 1145-1152.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 15 Aleil B, Ravanat C, Cazenave JP. et al. Flow cytometric analysis of intraplatelet VASP phosphorylation for the detection of clopidogrel resistance in patients with ischemic cardiovascular diseases. J Thromb Haemost 2005; 03: 85-92.
    CrossrefPubMedGoogle Scholar
  • 16 Aszodi A, Pfeifer A, Ahmad M. et al. The vasodilator-stimulated phosphoprotein (VASP) is involved in cGMP- and cAMP-mediated inhibition of agonist-induced platelet aggregation, but is dispensable for smooth muscle function. Embo J 1999; 18: 37-48.
    CrossrefPubMedGoogle Scholar
  • 17 Hauser W, Knobeloch KP, Eigenthaler M. et al. Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice. Proc Natl Acad Sci U S A 1999; 96: 8120-8125.
    CrossrefPubMedGoogle Scholar
  • 18 Begonja AJ, Teichmann L, Geiger J. et al. Platelet regulation by NO/cGMP signaling and NAD(P)H oxidase-generated ROS. Blood cells, molecules & diseases 2006; 36: 166-170.
    CrossrefPubMedGoogle Scholar
  • 19 Massberg S, Gruner S, Konrad I. et al. Enhanced in vivo platelet adhesion in vasodilator-stimulated phosphoprotein (VASP)-deficient mice. Blood 2004; 103: 136-142.
    CrossrefPubMedGoogle Scholar
  • 20 Mangin P, Yuan Y, Goncalves I. et al. Signaling role for phospholipase C gamma 2 in platelet glycoprotein Ib alpha calcium flux and cytoskeletal reorganization. Involvement of a pathway distinct from FcR gamma chain and Fc gamma RIIA. J Biol Chem 2003; 278: 32880-32891.
    CrossrefPubMedGoogle Scholar
  • 21 Pula G, Schuh K, Nakayama K. et al. PKCdelta regulates collagen-induced platelet aggregation through inhibition of VASP-mediated filopodia formation. Blood 2006; 108: 4035-4044.
    CrossrefPubMedGoogle Scholar
  • 22 Gupton SL, Gertler FB. Filopodia: the fingers that do the walking. Sci STKE 2007; Sep 08 2007(400) re5.
    PubMedGoogle Scholar
  • 23 Savage B, Shattil SJ, Ruggeri ZM. Modulation of platelet function through adhesion receptors. A dual role for glycoprotein IIb-IIIa (integrin alpha IIb beta 3) mediated by fibrinogen and glycoprotein Ib-von Willebrand factor. J Biol Chem 1992; 267: 11300-11306.
    PubMedGoogle Scholar