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Thromb Haemost 2010; 103(02): 450-460
DOI: 10.1160/TH09-03-0165
Animal Models
Schattauer GmbH

Protective effect of ischaemic preconditioning on ischaemia/reper-fusion-induced microvascular obstruction determined by online measurements of coronary pressure and blood flow in pigs

Aniko Posa
1   Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
,
Noemi Pavo
2   Department of Cardiology, Medical University of Vienna, Vienna, Austria
,
Rayyan Hemetsberger
2   Department of Cardiology, Medical University of Vienna, Vienna, Austria
,
Csaba Csonka
3   Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary
,
Tamas Csont
3   Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary
,
Peter Ferdinandy
3   Cardiovascular Research Group, Department of Biochemistry, University of Szeged, Szeged, Hungary
,
Zsolt Petrasi
4   Institute of Diagnostics and Radiation Oncology, University of Kaposvar, Kaposvar, Hungary
,
Csaba Varga
1   Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
,
Imre J. Pavo
2   Department of Cardiology, Medical University of Vienna, Vienna, Austria
,
Ferenc Laszlo jr
5   Institute of Physical Education and Sport Sciences, University of Szeged, Szeged, Hungary
,
Kurt Huber
6   Department of Medicine (Cardiology and Emergency Medicine), Wilhelminenhospital, Vienna, Austria
,
Mariann Gyöngyösi
2   Department of Cardiology, Medical University of Vienna, Vienna, Austria
› Author Affiliations Financial support: This study was supported by the Stiftung Aktion Österreich-Ungarn (OMAA 59üu14), Verein zur Förderung der Forschung – ATVB, Verein zur Förderung der Forschung im Bereich der experimentellen und klinischen Kardiologie and B&P Research GmbR. We thank Christoph Felsinger and the Atomed GmbH for providing the high-pressure injector for the presented experiments.
Further Information

Publication History

Received: 14 March 2009

Accepted after major revision: 25 October 2009

Publication Date:
22 November 2017 (online)

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Summary

We investigated the protective effect of ischaemic preconditioning (IP) on the maintenance of coronary patency using online measurements of coronary pressures and blood flow in a closed-chest reperfused acute myocardial infarction (MI) model in pigs. Catheter-based 90-min occlusion followed by 60-min reperfusion of the left anterior descending coronary artery (LAD) was performed in anesthetised pigs (MI group). IP was applied (IP group) through two cycles of 5-min occlusion and 5-min reperfusion of the LAD before MI induction. Coronary patency was determined by measurements of coronary wedge pressure, collateral fractional flow reserve (FFRcoll), collateral pressure index (CPI) and absolute coronary blood flow (CBF). Inducible and constitutive nitric oxide synthase (iNOS/cNOS) activities and expressions were determined in the myocardium. Plasma levels of myeloperoxidase (MPO, index of activated leukocytes) and mean platelet volume (MPV, index of activated platelets) were measured. IP resulted in significantly lower levels of MPO (0.52 ± 0.19 vs. 1.05 ± 0.24 U/l, p<0.001) and MPV (9.1 ± 0.6 vs. 9.6 ± 1.0 fl, p=0.04), higher FFRcoll (0.17 ± 0.05 vs. 0.04 ± 0.05, p<0.001), CPI (0.13 ± 0.05 vs. 0.02 ± 0.05, p<0.001) and CBF (70.7 ± 4.2 vs. 50.8 ± 4.8 m/min, p<0.001) post-reperfusion as compared with the MI group. IP resulted in significantly higher cNOS activity and eNOS expression. Significant negative correlation was found between MPO and measures of coronary patency (FFRcoll, CPI and CBF) and cNOS activity. Moreover, cNOS activity correlated significantly with FFRcoll, CPI and CBF. In conclusion, IP attenuates the release of MPO and platelet activation, thereby contributing to the maintenance of vessel patency at microvascular level after reperfusion of the infarct-related artery.

Keywords

Nitric oxide - ischaemia/reperfusion - collateral circulation
 

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