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Thromb Haemost 2009; 101(05): 911-915
DOI: 10.1160/TH08-06-0399
Platelets and Blood Cells
Schattauer GmbH

Influence of platelet count on the expression of platelet collagen receptor glycoprotein VI (GPVI) in patients with acute coronary syndrome

Boris Bigalke
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Konstantinos Stellos
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Dimitrios Stakos
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Thomas Joos
2   Biochemistry Department, NMI – Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany
,
Oliver Pötz
2   Biochemistry Department, NMI – Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany
,
Tobias Geisler
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Christian Bischofs
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Elisabeth Kremmer
3   Institute for Molecular Immunology, GSF-National Research Center for Environment and Health, Helmoltz Center, Munich, Germany
,
Björn F. Krämer
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Peter Seizer
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Andreas E. May
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Stephan Lindemann
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
,
Meinrad Gawaz
1   Medizinische Klinik, Klinik für Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany
› Author AffiliationsFinancial support: The study was supported by grants of Deutsche Forschungsgemeinschaft (Li849/3–1 to S.L., MA2186/3–1 and GK794 to M.G.), Karl-und-Lore-Klein-Stiftung (D.30.08886), AKF-Program (No.231–0–0), Karl-Kuhn-Stiftung, and Wilhelm-Sanders-Stiftung (Nr.2003.0601).
Further Information

Publication History

Received: 22 June 2008

Accepted after major revision: 20 January 2009

Publication Date:
24 November 2017 (online)

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Summary

Platelets play a key role in the development of an acute coronary syndrome (ACS) and contribute to cardiovascular events. Platelet collagen receptor glycoprotein VI (GPVI) contributes significantly to platelet adhesion and thrombus formation in ACS. We consecutively investigated both the platelet count and the platelet surface expression of GPVI in 843 patients with a symptomatic coronary artery disease verified by coronary angiography. Four hundred fourteen patients presented with stable angina pectoris and 429 patients with ACS. Platelet surface expression of GPVI and CD62P was determined by flow cytometry and platelet count with a coulter counter, plasmatic soluble GPVI was measured by ELISA. Platelet GPVI expression in patients with ACS was compared to platelet count. Patients with ACS showed significantly elevated GPVI expression levels in the first and second quartiles of platelet count compared to patients with higher platelet count [mean fluorescence intensity (MFI) ± standard deviation): 1st vs. 4th: 20.44 ± 6.1 vs. 18.62 ± 3.7; p=0.012; 2ndvs.3rd:21.2±8.5vs.18.76±3.7;P=0.03; 2ndvs.4th: 21.2±8.5vs.18.62±3.7;P=0.004], which was paralleled in trend for the CD62P expression [MFI: 1st vs. 4th: 11.2 ± 6.8 vs. 12.3 ± 9; p=0.057; 2nd vs. 3rd: 16.3 ± 16 vs.12.7 ± 5.3; p=0.138; 2nd vs. 4th: 16.3 ± 16 vs.11 ± 4.4; p=0.043]. In a subgroup of 48 patients with ACS, determination of soluble GPVI showed similar results [plasma GPVI (ng/ml): 1stvs.4th: 1.6 ± 0.6 vs. 1.2 ± 0.4; p=0.046; 1st vs. 3rd: 1.6 ± 0.6 vs. 1.1 ± 0.5; p=0.038; 2nd vs. 3rd: 1.9 ± 0.8 vs. 1.1 ± 0.5; p=0.04; 2nd vs. 4th: 1.9 ± 0.8 vs. 1.2 ± 0.4; p=0.056]. Thus, a lower platelet count comes along with a higher GPVI surface expression and plasma concentration in patients with ACS, which potentially reflects increased activation and enhanced recruitment of platelets to the site of vascular injury.

Keywords

Platelet count - glycoprotein VI - acute coronary syndrome
 

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