Effects of von Willebrand factor concentration and platelet collision on shear-induced platelet activation
Zhenyue Gao
1
Bioengineering Department of Beijing University of Aeronautics and Astronautics, Beijing, China
,
Fang Liu
2
Jiangsu Institute of Hematology, Suzhou, China
,
Ziqiang Yu
2
Jiangsu Institute of Hematology, Suzhou, China
,
Xia Bai
2
Jiangsu Institute of Hematology, Suzhou, China
,
Chun Yang
1
Bioengineering Department of Beijing University of Aeronautics and Astronautics, Beijing, China
,
Fengyuan Zhuang
1
Bioengineering Department of Beijing University of Aeronautics and Astronautics, Beijing, China
,
Changgeng Ruan
2
Jiangsu Institute of Hematology, Suzhou, China
› InstitutsangabenFinancial support: This work was supported by a grant from the National Natural Sciences Foundation of PR China (No. 10402002 and 30670904), and a Foundation for the Author of National Excellent Doctoral Dissertation of PR China (No. 200356).
The binding of plasma von Willebrand factor (vWF) to platelet glycoprotein (GP) Ibα in a high shear stress field, and subsequent integrin-GPIIb/IIIa-vWF conjunction induces platelet aggregation (SIPA). However, the specific biomechanical mechanism of the vWF-GPIb interaction still remains to be elucidated. A parallel-plate rectangular flow chamber was built to simulate a stenopeic artery flow pattern. Using the flow chamber, we examined shear- induced platelet activation (SIPAct) at different vWF concentrations (5–25 µg/ml) and several simulated stenotic high shear rates. P-selectin expression on the platelets and annexin V binding to the platelets were used as two markers of platelet activation. At different localized shear rates (3,000 s-1–9,500 s-1), the percentage of annexin V and P-selectin positive cells increased from 8.3 ± 0.4% to 22.3 ± 1.8% ( p 0.05) and from 17.4 ± 0.5% to 33.5 ± 2.5% (p 0.05),respectively. As the vWF concentration increased from 5 µg/ml to 25 µg/ml, the annexinV binding rate increased from 7.2 ± 0.6% to 53.4 ± 3.8% (p 0.05), and P-selectin expression increased from 16.5 ± 1.2% to 65.9 ± 5.2% (p 0.05). A test in a uniform shear field using cone-plate viscometer rheometry showed that the platelet activation rate was proportional to the platelet concentration. This result suggests that platelet collision is one of the impact factors of SIPAct.
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