Thromb Haemost 2006; 95(05): 886-892
DOI: 10.1160/TH06-02-0100
New Technologies, Diagnostic Tools and Drugs
Schattauer GmbH

ADAMTS13-binding IgG are present in patients with thrombotic thrombocytopenic purpura

Han-Mou Tsai
1   Division of Hematology, Montefiore Medical Center and
,
Mojgan Raoufi
2   Department of Pathology, Albert Einstein College of Medicine, Bronx, New York
,
Wenhua Zhou
1   Division of Hematology, Montefiore Medical Center and
,
Enriqueta Guinto
3   American Diagnostica Inc., Stamford, Connecticut; USA
,
Nickolas Grafos
3   American Diagnostica Inc., Stamford, Connecticut; USA
,
Safi Ranzurmal
3   American Diagnostica Inc., Stamford, Connecticut; USA
,
Robert S. Greenfield
3   American Diagnostica Inc., Stamford, Connecticut; USA
,
Jacob H. Rand
1   Division of Hematology, Montefiore Medical Center and
2   Department of Pathology, Albert Einstein College of Medicine, Bronx, New York
› Institutsangaben
Financial support: This study was supported in part by grants R01 HL62136 and R01 HL72876 (H-MT) from the National Heart Lung and Blood Institute of the National Institutes of Health.
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Publikationsverlauf

Received 17. Februar 2006

Accepted after revision 27. März 2006

Publikationsdatum:
01. Dezember 2017 (online)

Summary

Functional assays are commonly used to measure the antibodies of ADAMTS13 found in patients of thrombotic thrombocytopenic purpura (TTP). In this study we used an enzyme-linked immunoassay to analyze the ADAMTS13-binding IgG levels in six groups of individuals: normal, random hospitalized patients, acute TTP,TTP after receiving plasma therapy,TTP in remission, and other types of thrombotic microangiopathy (TMA).The results showed that ADAMTS13-binding IgG levels were elevated in 100% of the acute TTP group, 75% of the TTP group after receiving plasma therapy, and 40% of the remission group. Overall, the ADAMTS13-binding IgG levels correlated with the inhibitory activity levels againstADAMTS13 (r = -0.69,P<0.0001).The assay also detected elevated IgG binding levels in 5% - 15% of the normal, random, and other TMA control groups.Addition of purified ADAMTS13 protein to the plasma samples suppressed the IgG binding in each of the acute TTP patients, but in none of the non-TTP groups. Serial measurement in a patient that had two exacerbations of TTP within the first three weeks revealed that the ADAMTS13 activity levels remained < 0.1 U/ml during this period, and the ADAMTS13-binding IgG remained elevated, suggesting that ADAMTS13 analysis may provide valuable insight to the disease status during the course of therapy. Analysis of ADAMTS13-binding IgG is helpful for the diagnosis and management of TTP.

 
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