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Thromb Haemost 2005; 93(01): 106-114
DOI: 10.1160/TH04-06-0340
Platelets and Blood Cells
Schattauer GmbH

RGD-modified liposomes targeted to activated platelets as a potential vascular drug delivery system

Anirban Sen Gupta
1   Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA
,
Guofeng Huang
1   Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA
,
Brian J. Lestini
1   Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA
,
Sharon Sagnella
1   Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA
,
Kandice Kottke-Marchant
2   Department of Clinical Pathology, Cleveland Clinic Foundation, Cleveland, Ohio, USA
,
Roger E. Marchant
1   Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA
› Author AffiliationsFinancial support: This study was supported by a research grant: NIH HL –70263.
Further Information

Publication History

Received 03 June 2004

Accepted after resubmission 01 November 2004

Publication Date:
14 December 2017 (online)

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Summary

Local drug delivery has become an important treatment modality for the prevention of thrombotic events following coronary angioplasty. In this study, we investigate the ability of liposomes bearing surface conjugated linear Arg-Gly-Asp (RGD) peptide (GSSSGRGD SPA) moieties to target and bind activated platelets, and the effect of such RGD-modified liposomes on platelet activation and aggregation. The binding of RGD-liposomes to human platelets was assessed by fluorescence microscopy,phase contrast microscopy and flow cytometry. The effect of RGDmodified liposomes on platelet activation and aggregation was investigated in vitro, with and without platelet agonists. RGD-liposomes were found to bind activated platelets at levels significantly greater than the control RGE-liposomes.The RGD-liposomes did not exhibit any statistically significant effect on platelet activation or aggregation.The results demonstrate the ability of the RGD-modified liposomes to target and bind activated platelets without causing significant platelet aggregation and suggests a feasible way for the development of a platelet-targeted anti-thrombogenic drug delivery system. Furthermore, the approach can be extended to the development of liposomes for other vascular targets, for application in drug delivery or gene therapy.

Keywords

Liposomes - RGD-modification - platelet-targeted - drug delivery
 

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