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DOI: 10.1055/s-2008-1081357
© Georg Thieme Verlag KG Stuttgart · New York
Withanolide Z, a New Chlorinated Withanolide from Withania somnifera
Publication History
Received: March 22, 2008
Revised: July 9, 2008
Accepted: July 11, 2008
Publication Date:
05 November 2008 (online)
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Abstract
Reverse-phase preparative HPLC analysis of the n-butanol fraction of the methanolic extract of Withania somnifera Dunal (leaves) afforded a novel chlorinated withanolide, namely withanolide Z (1), along with four known withanolides, withanolide B (2), withanolide A (3), 27-hydroxywithanolide B (4) and withaferin A (5). Their structures were elucidated by IR, MS, CD and a combination of 1 D and 2 D NMR spectral analyses. The Leishmania donovani DNA topoisomerase I inhibitory activities of the isolated compounds were determined.
Key words
Solanaceae - Withania somnifera - withanolide - HPLC - Leishmania donovani DNA topoisomerase I
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Dr. Sibabrata Mukhopadhyay
Medicinal Chemistry Division
Indian Institute of Chemical Biology
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