Planta Med 1980; 39(8): 375-382
DOI: 10.1055/s-2008-1074932
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Protoplast-derived Clones from Cell Cultures of Digitalis purpurea

B. Diettrich1 , D. Neumann2 , M. Luckner1
  • 1Martin-Luther-University, Section of Pharmacy, Halle, German Democratic Republic
  • 2Institute of Plant Biochemistry, Academy of Sciences of the German Democratic Republic, Halle
Further Information

Publication History

Publication Date:
29 April 2008 (online)

Abstract

Protoplasts were obtained with high yield from cells of suspension cultures of Digitalis purpurea by incubation with a mixture of cellulase R 10 Onozuka and macerozyme RIO. They were cultivated in a solidified, conditioned Murashige-Skoog medium with 30 μM kinetin and 0.3 M mannitol. Within 24 hours the protoplasts regenerated walls. These single cells underwent subsequent divisions forming clusters of 30-40 cells within 4 weeks. The protoplast-derived cells, as well as the cells of the suspension cultures used for protoplast preparation, obviously divide by means of a centripetal mechanism, deviating from the centrifugal one typical for cells of higher plants.