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Planta Med 2008; 74(8): 794-801
DOI: 10.1055/s-2008-1074550
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Comparison of the Phytohormones Genistein, Resveratrol and 8-Prenylnaringenin as Agents for Preventing Osteoporosis

Stephan Sehmisch1 , Frauke Hammer1 , Julie Christoffel2 , Dana Seidlova-Wuttke2 , Mohammad Tezval1 , Wolfgang Wuttke2 , Klaus Michael Stuermer1 , Ewa Klara Stuermer1
  • 1Department of Trauma and Reconstructive Surgery, University of Goettingen, Goettingen, Germany
  • 2Department of Clinical and Experimental Endocrinology, University of Goettingen, Goettingen, Germany
Further Information

Publication History

Received: January 6, 2008 Revised: April 18, 2008

Accepted: April 22, 2008

Publication Date:
06 June 2008 (online)

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Abstract

As the average age of society increases, identifying and preventing osteoporosis becomes more important. According to the results of the Women’s Health Initiative study, substitution of estradiol is not recommended in hormone replacement therapy (HRT), although phytoestrogens might be a safe alternative. In this study, the osteoprotective effects of genistein (Gen), resveratrol (Res) and 8-prenylnaringenin (8PN) were evaluated by analysing bone biomechanical strength and bone mineral density. After ovariectomy, 88 female rats received soy-free food (C), and according to their grouping, were fed estradiol (E), GEN, RES or 8PN for 12 weeks. The phytohormones were given in two dosages. To analyse the osteoprotective effects of the tested substances, bone biomechanical properties and bone mineral density (BMD) were evaluated on the upper tibial metaphysis. Bone biomechanical properties were significantly improved after treatment with E (Fmax: 90.6 N) and 8PN (85.0 N) compared to GEN (76.0 N), RES (72.6 N) and C (76.6 N). Bone biomechanical properties with 8PN (yL: 55.7 N) supplementation reached a level similar to that seen after E (49.3 N) supplementation. Treatment with GEN (38.5 N) was not as effective as E and 8PN, but demonstrated improved biomechanical properties compared to C (40.1 N) and RES (36.3 N). E (Cn.Dn. 217 mg/cm3) and 8PN (165 mg/cm3) showed superior results in the analysis of bone mineral density compared to C (112 mg/cm3). GEN (164 mg/cm3) also demonstrated superior results, though not as good as E and 8PN. RES (124 mg/cm3) revealed no effect on bone density. Treatment with 8PN resulted in very good biomechanical properties and showed an increased BMD. GEN had a smaller effect on bone biomechanical strength, while RES did not have an effect on bone biomechanical strength or BMD. Therefore, 8PN might be a safe alternative for HRT, but further studies are needed.

Abbreviations

BMD:total bone mineral density

C:control

Cn.Dn.:cancellous bone density

Ct.Dn.:cortical bone density

E:estradiol

fL:failure load

Fmax:maximum load

GEN:genistein

HRT:hormone replacement therapy

8PN:8-prenylnaringenin

RES:resveratrol

S:stiffness

yL:yield load

Key words

osteoporosis - biomechanical testing - 8-prenylnaringenin - genistein - resveratrol

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Dr. Stephan Sehmisch

Department of Trauma and Reconstructive Surgery

University of Goettingen

Robert Koch-Str. 40

37075 Goettingen

Germany

Phone: +49-551-39-2462

Fax: +49-551-39-8981

Email: stephan.sehmisch@med.uni-goettingen.de