Planta Med 2008; 74(5): 509-514
DOI: 10.1055/s-2008-1074499
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Plant polyphenols against UV-C-induced Cellular Death

Vladimir Kostyuk1 , 2 , Alla Potapovich1 , 2 , Tatiana Suhan2 , Chiara De Luca1 , Giovanna Pressi3 , Roberto Dal Toso3 , Liudmila Korkina1
  • 1Dermatology Research Hospital (Istituto Dermopatico dell'Immacolata, IDI IRCCS), Rome, Italy
  • 2Biology Department, Byelorussian State University, Minsk, Belarus
  • 3Institute for Biotechnological Research (IRB S.r.l.), Altavilla Vicentina, Italy
Further Information

Publication History

Received: November 28, 2007 Revised: February 27, 2008

Accepted: March 1, 2008

Publication Date:
10 April 2008 (online)

Abstract

The glycosylated phenylpropanoid verbascoside isolated from cultured cells of the medicinal plant Syringa vulgaris (Oleaceae) has previously been characterized as an effective scavenger of biologically active free radicals such as hydroxyl, superoxide, and nitric oxide, as a chelator of redox active transition metal ions (Fe2+, Fe3+, Cu2+, and Ni2+), and an inhibitor of lipid peroxidation. In the present work, we have compared the cytoprotective effects of the biotechnologically produced verbascoside with two commercially available polyphenols (the glycosylated flavonoid rutin and its aglycone quercetin) against free radical-mediated UVC-induced cellular death in cultures of human keratinocytes (HaCaT) and breast cancer cells (MCF 7). We have shown that all the polyphenols studied afforded effective protection against UVC-induced necrosis and did not prevent UVC-induced apoptosis in both normal and tumor cell lines. The cytoprotection did not correlate either with UVC absorbance by polyphenols or with their superoxide radical scavenging properties. However, UVC protection strongly depended on the lipid peroxidation inhibiting and Fe2+ chelating properties of polyphenols. We suggest that these plant polyphenols could be feasible for a photoprotection of human skin.

Abbreviations

AO:acridine orange

DMEM:Dulbecco’s modified Eagle’s medium

EB:ethidium bromide

EDTA:ethylenediaminetetraacetic acid

FCS:fetal calf serum

GST:glutathione S-transferase

LDH:lactate dehydrogenase

MTT:3-(4,5-dimethylthiazol-2-yl)-2,5-diphe- nyltetrazolium bromide

TBARS:thiobarbituric acid reactive substances

UV-C:ultraviolet C

VB:verbascoside

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Prof. Dr. Vladimir Kostyuk

Laboratory of Tissue Engineering & Cutaneous Physiopathology

Dermatology Research Hospital (Istituto Dermopatico dell’Immacolata, IDI IRCCS)

Via Monti di Creta 104

00167 Rome 00167

Italy

Phone: +39-06-911-2193

Fax: +39-06-6646-4253

Email: u.kastsiuk@idi.it