Horm Metab Res 2008; 40(3): 214-218
DOI: 10.1055/s-2008-1046796
Humans, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Modulation of Adipocytokines Response and Weight Loss Secondary to a Hypocaloric Diet in Obese Patients by -55CT Polymorphism of UCP3 Gene

D. A. de Luis 1 , R. Aller 1 , O. Izaola 1 , M. G. Sagrado 1 , R. Conde 1
  • 1Institute of Endocrinology and Nutrition, Medicine School and Unit of Investigation, Hospital Rio Hortega, University of Valladolid, Valladolid, Spain-RD 056/0013
Further Information

Publication History

received 23.03.2007

accepted 06.11.2007

Publication Date:
17 March 2008 (online)

Abstract

Decreased expression or function of UCP3 (uncoupling protein 3) could reduce energy expenditure and increase the storage of energy as fat. Some studies have pointed to a role of UCP3 in the regulation of whole body energy homeostasis, diet induced obesity, and regulation of lipids as metabolic substrates. The C/C genotype of a polymorphism in the UCP3 promoter (-55C→T) is associated with an increased expression of UCP3 mRNA in muscle. The aim of our study was to investigate the influence of -55CT polymorphism of UCP3 gene on adipocytokines response and weight loss secondary to a hypocaloric diet in obese patients. A population of 107 obese (body mass index >30) nondiabetic outpatients was analyzed in a prospective way. Before and after three months of a hypocaloric diet, an indirect calorimetry, tetrapolar electrical bioimpedance, blood pressure, a serial assessment of nutritional intake with 3-day written food records, and biochemical analysis were performed. The lifestyle modification program consisted of a hypocaloric diet (1520 kcal, 52% of carbohydrates, 25% of lipids and 23% of proteins). The exercise program consisted of aerobic exercise for at least 3 times per week (60 minutes each). The mean age was 49.5±34.5 years and the mean BMI 34.5±4.8, with 27 males (25.3%) and 80 females (74.7%). Ninety patients (25 males/65 females) (83.6%) had the genotype 55CC (wild group) and 17 patients (2 male/15 females) (16.4%) 55CT (mutant group). The percentage of responders (weight loss) was similar in both groups (wild group: 84.7% vs. mutant group: 81.8%). BMI, weight, fat mass, systolic blood pressure, LDL cholesterol, waist circumference, and waist-to-hip ratio decreased in the wild group and RMR and VO2 were increased. In the mutant group, BMI and weight decreased. Leptin and IL-6 levels have a significant decrease in the wild group (9.6%: p<0.05) and (30.5%: p<0.05), respectively. Patients with -55CC genotype have a significant decrease in leptin, interleukin 6, BMI, weight, fat mass, systolic blood pressure, LDL cholesterol, waist circumference, waist-to-hip ratio weight, fat mass, and systolic blood pressure.

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Correspondence

Dr. D. A. de Luis

Professor Associated of Nutrition

Executive Director of Institute of Endocrinology and Nutrition

Medicine School

Valladolid University

C/Los perales 16

Simancas 47130

Valladolid

Spain

Phone: +34/98/342 04 00

Fax: +34/98/333 15 66

Email: dadluis@yahoo.es