Pneumologie 2009; 63(1): 14-22
DOI: 10.1055/s-2008-1038292
Serie: Infektiologie

© Georg Thieme Verlag KG Stuttgart · New York

Nachweis von Atemwegsviren – Wie, warum, wann und wo?

Detection of Respiratory Viruses – How, When, Where and Why?G.  Rohde1 , C.  Drosten2 , I.  Borg1 , B.  Hauptmeier1 , F.  Ringshausen1 , G.  Schultze-Werninghaus1 , K.  Überla3
  • 1Medizinische Klinik III – Pneumologie, Allergologie und Schlaf- und Beatmungsmedizin, Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil GmbH, Bochum
  • 2Institut für Virologie, Universitätsklinikum Bonn
  • 3Abteilung für Molekulare und Medizinische Virologie, Ruhr-Universität Bochum
Further Information

Publication History

Publication Date:
09 January 2009 (online)

Zusammenfassung

Atemwegsviren sind für die überwiegende Mehrzahl aller so genannten Erkältungskrankheiten und grippalen Infekte in der kalten Jahreszeit, akuter respiratorischen Erkrankungen im Kindesalter sowie akuter Exazerbationen von Asthma bronchiale und chronisch obstruktiver Lungenerkrankungen (COPD) verantwortlich und spielen eine nicht zu vernachlässigende Rolle bei der ambulant erworbenen Pneumonie. Leider ist der Nachweis von Atemwegsviren bis heute schwierig. In dieser Übersicht werden daher die wesentlichen Untersuchungsverfahren vorgestellt und der aktuelle Stand des Wissens über die klinische Bedeutung von Atemwegsviren bei den unterschiedlichen Erkrankungen präsentiert.

Abstract

Respiratory viruses trigger the majority of common colds, acute respiratory illnesses in children during the cold season as well as acute exacerbations of asthma and chronic obstructive pulmonary disease. They also play a role in community acquired pneumonia. Unfortunately their detection is still difficult. The aim of this review is therefore to introduce the methods of detection and to present the current knowledge of the clinical role of respiratory viruses in different diseases.

Literatur

  • 1 Modrow S, Falke D, Truyen U. Labormethoden zum Nachweis von Virusinfektionen. Molekulare Virologie. 2. ed. Heidelberg-Berlin: Spektrum Akademischer Verlag GmbH 2003: 114-129
  • 2 Marshall D J, Reisdorf E, Harms G. et al . Evaluation of a Multiplexed PCR Assay for Detection of Respiratory Viral Pathogens in a Public Health Laboratory Setting.  J Clin Microbiol. 2007;  45 3875-3882
  • 3 Borg I, Rohde G, Loeseke S. et al . Evaluation of a quantitative real-time PCR for the detection of RSV in pulmonary diseases.  Eur Respir J. 2003;  21 944-951
  • 4 Ieven M. Currently used nucleic acid amplification tests for the detection of viruses and atypicals in acute respiratory infections.  J Clin Virol. 2007;  40 259-276
  • 5 Stang A, Korn K, Wildner O. et al . Characterization of Virus Isolates by Particle-Associated Nucleic Acid PCR.  J Clin Microbiol. 2005;  43 716-720
  • 6 Ambrose H E, Clewley J P. Virus discovery by sequence-independent genome amplification.  Rev Med Virol. 2006;  16 365-383
  • 7 Janeway Jr C A, Travers P, Walport M. et al .D. Structure of the antibody molecule and the immunoglobulin genes. In: Janeway Jr. CA (ed). Immunobiology: the immune system in health and disease. 4. ed. London/New York: Elsevier Science Ltd/Garland Publishing 1999: 79-113
  • 8 Xiang X, Qiu D, Chan K P. et al . Comparison of three methods for respiratory virus detection between induced sputum and nasopharyngeal aspirate specimens in acute asthma.  J Virol Methods. 2002;  101 127-133
  • 9 Beckham J D, Cadena A, Lin J. et al . Respiratory viral infections in patients with chronic, obstructive pulmonary disease.  J Infect. 2005;  50 322-330
  • 10 Monto A S, Ullman B M. Acute respiratory illness in an American community. The Tecumseh study.  JAMA. 1974;  227 164-169
  • 11 Macfarlane J, Holmes W, Gard P. et al . Prospective study of the incidence, aetiology and outcome of adult lower respiratory tract illness in the community.  Thorax. 2001;  56 109-114
  • 12 Meza R A, Bridges-Webb C, Sayer G P. The management of acute bronchitis in general practice: results from the Australian Morbidity and Treatment Survey, 1990 – 1991.  Aust Fam Physician. 1994;  23 1550-1553
  • 13 Rohde G, Wiethege A, Borg I. et al . Respiratory viruses in exacerbations of chronic obstructive pulmonary disease requiring hospitalization – a case-control study.  Thorax. 2003;  58 37-42
  • 14 Forster J, Ihorst G, Rieger C H. et al . Prospective population-based study of viral lower respiratory tract infections in children under 3 years of age (the PRI.DE study).  Eur J Pediatr. 2004;  163 709-716
  • 15 Hoek L van der, Sure K, Ihorst G. et al . Croup is associated with the novel coronavirus NL63.  PLoS Med 2005. Aug ;  2 (8) e240
  • 16 Yap F H, Ho P L, Lam K F. et al . Excess hospital admissions for pneumonia, chronic obstructive pulmonary disease, and heart failure during influenza seasons in Hong Kong.  J Med Virol. 2004;  73 617-623
  • 17 Sethi S. Infectious etiology of acute exacerbations of chronic bronchitis.  Chest. 2000;  117 380S-385S
  • 18 Smith C B, Golden C A, Kanner R E. et al . Association of viral and Mycoplasma pneumoniae infections with acute respiratory illness in patients with chronic obstructive pulmonary diseases.  Am Rev Respir Dis. 1980;  121 225-232
  • 19 Gump D W, Phillips C A, Forsyth B R. et al . Role of infection in chronic bronchitis.  Am Rev Respir Dis. 1976;  113 465-474
  • 20 Buscho R O, Saxtan D, Shultz P S. et al . Infections with viruses and Mycoplasma pneumoniae during exacerbations of chronic bronchitis.  J Infect Dis. 1978;  137 377-383
  • 21 Seemungal T, Harper-Owen R, Bhowmik A. et al . Respiratory Viruses, Symptoms, and Inflammatory Markers in Acute Exacerbations and Stable Chronic Obstructive Pulmonary Disease.  Am J Respir Crit Care Med. 2001;  164 1618-1623
  • 22 Hutchinson A F, Ghimire A K, Thompson M A. et al . A community-based, time-matched, case-control study of respiratory viruses and exacerbations of COPD.  Respir Med. 2007;  101 2472-2481
  • 23 Rohde G, Borg I, Arinir U. et al . Relevance of human metapneumovirus in exacerbations of COPD.  Respir Res. 2005;  6 : 150 doi 0.1186/1465-9921 – 6-150
  • 24 Hamelin M E, Cote S, Laforge J. et al . Human metapneumovirus infection in adults with community-acquired pneumonia and exacerbation of chronic obstructive pulmonary disease.  Clin Infect Dis. 2005;  41 498-502
  • 25 Martinello R A, Esper F, Weibel C. et al . Human metapneumovirus and exacerbations of chronic obstructive pulmonary disease.  J Infect. 2006;  53 248-254
  • 26 Wilkinson T M, Donaldson G C, Johnston S L. et al . Respiratory Syncytial Virus, Airway Inflammation and FEV1 Decline in Patients with COPD.  Am J Respir Crit Care Med. 2006;  173 871-876
  • 27 Matsuse T, Hayashi S, Kuwano K. et al . Latent adenoviral infection in the pathogenesis of chronic airways obstruction.  Am Rev Respir Dis. 1992;  146 177-184
  • 28 Retamales I, Elliott W M, Meshi B. et al . Amplification of inflammation in emphysema and its association with latent adenoviral infection.  Am J Respir Crit Care Med. 2001;  164 469-473
  • 29 McManus T E, Marley A M, Baxter N. et al . Acute and latent adenovirus in COPD.  Respir Med. 2007;  101 2084-2090
  • 30 Papadopoulos N G, Xepapadaki P, Mallia P. et al . Mechanisms of virus-induced asthma exacerbations: state-of-the-art. A GA(2)LEN and InterAirways document.  Allergy. 200 726;  62 457-470
  • 31 Johnston S L, Pattemore P K, Sanderson G. et al . Community study of role of viral infections in exacerbations of asthma in 9 – 11 year old children.  BMJ. 1995;  310 1225-1229
  • 32 Nicholson K G, Kent J, Ireland D C. Respiratory viruses and exacerbations of asthma in adults.  BMJ. 1993;  307 982-986
  • 33 Johnston S L, Pattemore P K, Sanderson G. et al . The relationship between upper respiratory infections and hospital admissions for asthma: A time-trend analysis.  Am J Respir Crit Care Med. 1996;  154 654-660
  • 34 Grunberg K, Timmers M C, de Klerk E P. et al . Experimental rhinovirus 16 infection causes variable airway obstruction in subjects with atopic asthma.  Am J Respir Crit Care Med. 1999;  160 1375-1380
  • 35 Papadopoulos N G, Bates P J, Bardin P G. et al . Rhinoviruses infect the lower airways.  J Infect Dis. 2000;  181 1875-1884
  • 36 Corne J M, Marshall C, Smith S. et al . Frequency, severity, and duration of rhinovirus infections in asthmatic and non-asthmatic individuals: a longitudinal cohort study.  Lancet. 2002;  359 831-834
  • 37 Wark P A, Johnston S L, Bucchieri F. et al . Asthmatic bronchial epithelial cells have a deficient innate immune response to infection with rhinovirus.  J Exp Med. 2005;  201 937-947
  • 38 de Roux A, Marcos M A, Garcia E. et al . Viral community-acquired pneumonia in nonimmunocompromised adults.  Chest. 2004;  125 1343-1351
  • 39 Angeles M M, Camps M, Pumarola T. et al . The role of viruses in the aetiology of community-acquired pneumonia in adults.  Antivir Ther. 2006;  11 351-359
  • 40 Dowell S F, Anderson L J, Gary Jr H E. et al . Respiratory syncytial virus is an important cause of community-acquired lower respiratory infection among hospitalized adults.  J Infect Dis. 1996;  174 456-462
  • 41 Ruiz M, Ewig S, Marcos M A. et al . Etiology of community-acquired pneumonia in hospitalized patients: Impact of age, comorbidity and severity.  Am J Respir Crit Care Med. 1999;  160 397-405
  • 42 Krech T, Wegmann T, Martin H. et al . Etiology of atypical pneumonias. A serological study on 1494 patients.  Schweiz Med Wochenschr. 1986;  116 2-7
  • 43 Farr B M, Kaiser D L, Harrison B D. et al . Prediction of microbial aetiology at admission to hospital for pneumonia from the presenting clinical features. British Thoracic Society Pneumonia Research Subcommittee.  Thorax. 1989;  44 1031-1035
  • 44 Dahmash N S, Chowdhury M N. Re-evaluation of pneumonia requiring admission to an intensive care unit: a prospective study.  Thorax. 1994;  49 71-76
  • 45 Bohte R, Furth R van, Broek P J van den. Aetiology of community-acquired pneumonia: a prospective study among adults requiring admission to hospital.  Thorax. 1995;  50 543-547
  • 46 Socan M, Marinic-Fiser N, Kraigher A. et al . Microbial aetiology of community-acquired pneumonia in hospitalised patients.  Eur J Clin Microbiol Infect Dis. 1999;  18 777-782
  • 47 La S B, Marrie T J, Auffray J P. et al . Mimivirus in pneumonia patients.  Emerg Infect Dis. 2005;  11 449-542
  • 48 Woo P CY, Lau S KP, Tsoi H w. et al . Clinical and Molecular Epidemiological Features of Coronavirus HKU1-Associated Community-Acquired Pneumonia.  The Journal of Infectious Diseases. 20 051;  192 1898-1907
  • 49 Cebular S, Lee S, Tolaney P. et al . Community-acquired pneumonia in immunocompromised patients. Opportunistic infections to consider in differential diagnosis.  Postgrad Med. 2003;  113 65-70, 73
  • 50 Duffau P, Moiton M P, Lafon M E. et al . A case of severe community acquired pneumonia and non-respiratory illness induced by Influenza A.  Med Mal Infect. 2006;  36 473-475
  • 51 Lee N, Rainer T H, Ip M. et al . Role of laboratory variables in differentiating SARS-coronavirus from other causes of community-acquired pneumonia within the first 72 h of hospitalization.  Eur J Clin Microbiol Infect Dis. 2006;  25 765-772
  • 52 Jennings L C, Anderson T P, Beynon K A. et al . Incidence and Characteristics of Viral Community-Acquired Pneumonia in Adults.  Thorax. 2008;  63 42-48
  • 53 Schlosser B, Rohde G, Borg I. et al . Viral and bacterial colonization in intubated patients in the ICU.  Proc Am Thorac Soc. 2005;  2 A822
  • 54 Daubin C, Parienti J J, Vincent S. et al . Epidemiology and clinical outcome of virus-positive respiratory samples in ventilated patients: a prospective cohort study.  Crit Care. 20 065;  10 R142
  • 55 Allander T, Tammi M T, Eriksson M. et al . Cloning of a human parvovirus by molecular screening of respiratory tract samples.  Proc Natl Acad Sci. 2005;  102 12 891-12 896
  • 56 Khetsuriani N, Kazerouni N N, Erdman D D. et al . Prevalence of viral respiratory tract infections in children with asthma.  Journal of Allergy and Clinical Immunology. 2007;  119 314-321
  • 57 Kleines M, Scheithauer S, Rackowitz A. et al . High Prevalence of Human Bocavirus Detected in Young Children with Severe Acute Lower Respiratory Tract Disease by Use of a Standard PCR Protocol and a Novel Real-Time PCR Protocol.  J Clin Microbiol. 2007;  45 1032-104
  • 58 Kupfer B, Vehreschild J, Cornely O. et al . Severe pneumonia and human bocavirus in adult.  Emerg Infect Dis. 2006;  12 1614-1616
  • 59 Allander T, Jartti T, Gupta S. et al . Human bocavirus and acute wheezing in children.  Clin Infect Dis. 2007;  44 904-910
  • 60 Chung J Y, Han T H, Kim S W. et al . Detection of viruses identified recently in children with acute wheezing.  J Med Virol. 2007;  79 1238-1243
  • 61 Fry A M, Lu X, Chittaganpitch M. et al . Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand.  J Infect Dis. 2007;  195 1038-1045
  • 62 Gendrel D, Guedj R, Pons-Catalano C. et al . Human bocavirus in children with acute asthma.  Clin Infect Dis. 2007;  45 404-405
  • 63 Kim Y J, Boeckh M, Englund J A. Community respiratory virus infections in immunocompromised patients: hematopoietic stem cell and solid organ transplant recipients, and individuals with human immunodeficiency virus infection.  Semin Respir Crit Care Med. 2007;  28 222-242
  • 64 Lee J I, Chung J Y, Han T H. et al . Detection of human bocavirus in children hospitalized because of acute gastroenteritis.  J Infect Dis. 2007;  196 994-997
  • 65 Lau S K, Yip C C, Que T L. et al . Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong.  J Infect Dis. 2007;  196 986-993
  • 66 Vicente D, Cilla G, Montes M. et al . Human bocavirus, a respiratory and enteric virus.  Emerg Infect Dis. 2007;  13 636-637
  • 67 Bastien N, Brandt K, Dust K. et al . Human Bocavirus infection, Canada.  Emerg Infect Dis. 2006;  12 848-850
  • 68 Kaplan N M, Dove W, Bu-Zeid A F. et al . Human bocavirus infection among children, Jordan.  Emerg Infect Dis. 2006;  12 1418-1420
  • 69 Neske F, Blessing K, Tollmann F. et al . Real-time PCR for diagnosis of human bocavirus infections and phylogenetic analysis.  J Clin Microbiol. 2007;  45 2116-2122
  • 70 Endo R, Ishiguro N, Kikuta H. et al . Seroepidemiology of human bocavirus in Hokkaido prefecture, Japan.  J Clin Microbiol. 2007;  45 3218-3223
  • 71 Allander T, Andreasson K, Gupta S. et al . Identification of a third human polyomavirus.  J Virol. 2007;  81 4130-4136
  • 72 Bialasiewicz S, Whiley D M, Lambert S B. et al . A newly reported human polyomavirus, KI virus, is present in the respiratory tract of Australian children.  J Clin Virol. 2007;  40 15-18
  • 73 Gaynor A M, Nissen M D, Whiley D M. et al . Identification of a novel polyomavirus from patients with acute respiratory tract infections.  PLoS Pathog. 2007;  3 e64
  • 74 Norja P, Ubillos I, Templeton K. et al . No evidence for an association between infections with WU and KI polyomaviruses and respiratory disease.  J Clin Virol. 2007;  40 307-311
  • 75 Englund J A. Diagnosis and epidemiology of community-acquired respiratory virus infections in the immunocompromised host.  Biol Blood Marrow Transplant. 2001;  7 2S-4S
  • 76 Couch R B, Englund J A, Whimbey E. Respiratory viral infections in immunocompetent and immunocompromised persons.  Am J Med. 1997;  102 2-9
  • 77 Barton T D, Blumberg E A. Viral pneumonias other than cytomegalovirus in transplant recipients.  Clin Chest Med. 2005;  26 707-720
  • 78 Duncan A J, Dummer J S, Paradis I L. et al . Cytomegalovirus infection and survival in lung transplant recipients.  J Heart Lung Transplant. 1991;  10 638-644
  • 79 Ettinger N A, Bailey T C, Trulock E P. et al . Cytomegalovirus infection and pneumonitis. Impact after isolated lung transplantation. Washington University Lung Transplant Group.  Am Rev Respir Dis. 1993;  147 1017-123
  • 80 Zamora M R, Davis R D, Leonard C. Management of cytomegalovirus infection in lung transplant recipients: evidence-based recommendations.  Transplantation. 2005;  80 157-163
  • 81 Humar A, Paya C, Pescovitz M D. et al . Clinical utility of cytomegalovirus viral load testing for predicting CMV disease in D+/R− solid organ transplant recipients.  Am J Transplant. 2004;  4 644-649
  • 82 Venard V, Carret A, Corsaro D. et al . Genotyping of adenoviruses isolated in an outbreak in a bone marrow transplant unit shows that diverse strains are involved.  J Hosp Infect. 2000;  44 71-74
  • 83 Ison M G. Adenovirus infections in transplant recipients.  Clin Infect Dis. 20 061;  43 331-339
  • 84 Runde V, Ross S, Trenschel R. et al . Adenoviral infection after allogeneic stem cell transplantation (SCT): report on 130 patients from a single SCT unit involved in a prospective multi center surveillance study.  Bone Marrow Transplant. 2001;  28 51-57
  • 85 Chakrabarti S, Mautner V, Osman H. et al . Adenovirus infections following allogeneic stem cell transplantation: incidence and outcome in relation to graft manipulation, immunosuppression, and immune recovery.  Blood. 2002;  100 1619-1627
  • 86 Baldwin A, Kingman H, Darville M. et al . Outcome and clinical course of 100 patients with adenovirus infection following bone marrow transplantation.  Bone Marrow Transplant. 2000;  26 1333-1338
  • 87 Shields A F, Hackman R C, Fife K H. et al . Adenovirus infections in patients undergoing bone-marrow transplantation.  N Engl J Med. 1985;  312 529-533
  • 88 Claas E C, Schilham M W, de Brouwer C S. et al . Internally controlled real-time PCR monitoring of adenovirus DNA load in serum or plasma of transplant recipients.  J Clin Microbiol. 2005;  43 1738-1744
  • 89 Kaiser L, Aubert J D, Pache J C. et al . Chronic rhinoviral infection in lung transplant recipients.  Am J Respir Crit Care Med. 2006;  174 1392-1399
  • 90 Nichols W G, Guthrie K A, Corey L. et al . Influenza infections after hematopoietic stem cell transplantation: risk factors, mortality, and the effect of antiviral therapy.  Clin Infect Dis. 2004;  39 1300-1306
  • 91 Small T N, Casson A, Malak S F. et al . Respiratory syncytial virus infection following hematopoietic stem cell transplantation.  Bone Marrow Transplant. 2002;  29 321-327
  • 92 Lee I, Barton T D. Viral respiratory tract infections in transplant patients: epidemiology, recognition and management.  Drugs. 2007;  67 1411-1427
  • 93 Kraaij M G van, Elden L J van, Loon A M van. et al . Frequent detection of respiratory viruses in adult recipients of stem cell transplants with the use of real-time polymerase chain reaction, compared with viral culture.  Clin Infect Dis. 2005;  40 662-669
  • 94 Rohde G. Therapeutic targets in respiratory viral infections.  Curr Med Chem. 2007;  14 2776-2782

Jun.-Prof. PD Dr. med. Gernot Rohde

Medizinische Klinik III, Pneumologie, Allergologie, Schlaf- und Beatmungsmedizin, Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil GmbH

Bürkle-de-la-Camp-Platz 1

44789 Bochum

Email: Gernot.Rohde@ruhr-uni-bochum.de

    >