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Int J Sports Med 1981; 02(2): 106-109
DOI: 10.1055/s-2008-1034592
© Georg Thieme Verlag Stuttgart · New York

Bioluminescent Assay for Determination of Picomole Amounts of AMP, ADP, and ATP in Adipose and Muscle Tissue Biopsies

M. Feraudi, J. Merkt, H. Weicker
  • Department of Internal Medicine, Institute of Pathophysiology and Sports Medicine, University of Heidelberg, FRG
The authors wish to thank Miss Carmen Gärtner for skillful technical assistance.
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Publikationsdatum:
14. März 2008 (online)

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Abstract

A method is described for quantitative determinations of AMP, ADP, and ATP in tissue samples weighing a few milligrams by utilizing constant bioluminescent light intensity after reagent mixing. The smallest measurable quantity of adenosine nucleotide amounts to 0.2 pmol (coefficient of variation = 0.05) in the bioluminescent assay.

Key words

AMP - ADP - ATP - bioluminescent assay - firefly luciferase - adipose tissue biopsy - muscle biopsy