Planta Med 2008; 74(4): 407-410
DOI: 10.1055/s-2008-1034330
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Anti-Infective and Cytotoxic Compounds Present in Blepharodon nitidum

José C. Aponte1 , Yannick Estevez2 , Robert H. Gilman3 , 4 , Walter H. Lewis5 , Rosario Rojas4 , Michel Sauvain2 , Abraham J. Vaisberg4 , Gerald B. Hammond1
  • 1Department of Chemistry, University of Louisville, Louisville, KY, U.S.A.
  • 2UMR 152 IRD - Université de Toulouse III, Mission IRD, Lima, Perú
  • 3Department of International Health, Johns Hopkins University School of Public Health, Baltimore, MD, U.S.A.
  • 4Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Perú
  • 5Department of Biology, Washington University, St. Louis, MO, U.S.A.
Further Information

Publication History

Received: December 1, 2007 Revised: January 27, 2008

Accepted: February 6,2008

Publication Date:
11 March 2008 (online)

Abstract

A pharmacological screening of the ethanol extract and fractions of Blepharodon nitidum led to the isolation of fourteen compounds, two of which, 24-hydroperoxycycloart-25-en-3β-ol and 25-hydroperoxycycloart-23-en-3β-ol, exhibited in vitro anti-Mycobacterium tuberculosis and antileishmanial activities, as well as significant cytotoxic activity against a panel of human tumor cell lines.

References

  • 1 Moura-Letts G, Villegas L F, Marçalo A, Vaisberg A J, Hammond G B. In Vivo wound-healing activity of oleanolic acid derived from the acid hydrolysis of Anredera diffusa. .  J Nat Prod. 2006;  69 978-9
  • 2 Rojas R, Caviedes L, Aponte J C, Vaisberg A J, Lewis W H, Lamas G. et al . Aegicerin, the first oleanane triterpene with wide-ranging antimycobacterial activity, isolated from Clavija procera. .  J Nat Prod. 2006;  69 845-6
  • 3 Neto C C, Vaisberg A J, Zhou B -N, Kingston D GI, Hammond G B. Cytotoxic triterpene acids from the Peruvian medicinal plant Polylepis racemosa. .  Planta Med. 2000;  66 483-4
  • 4 Kato T, Frei B, Heinrich M, Sticher O. Antibacterial hydroperoxysterols from Xanthosoma robustum. .  Phytochemistry. 1996;  41 1191-5
  • 5 Lee W B, Kwon H C, Cho O R, Lee K C, Choi S U, Baek N I. et al . Phytochemical constituents of Cirsium setidens Nakai and their cytotoxic against human cancer cell lines.  Arch Pharm Res. 2002;  25 628-35
  • 6 Wang J, Sporns P. MALDI-TOF MS analysis of food flavonol glycosides.  J Agric Food Chem. 2000;  48 1657-62
  • 7 Jautelat M, Grutzner J B, Roberts J D. Natural-abundance carbon-13 nuclear magnetic resonance spectra of terpenes and carotenes.  Proc Natl Acad Sci USA. 1970;  65 288-92
  • 8 Mallavadhani U V, Mahapatra A, Jamil K, Reddy P S. Antimicrobial activity of some pentacyclic triterpenes and their synthesized 3-O-lipophilic chains.  Biol Pharm Bull. 2004;  27 1576-9
  • 9 Mahato S, Kundu A. 13C NMR spectra of pentacyclic triterpenoids - A compilation and some salient features.  Phytochemistry. 1994;  37 1517-75
  • 10 Agrawal P K, Jain D C. 13C NMR spectroscopy of oleanane triterpenes.  Prog NMR Spectrom. 1992;  24 1-90.
  • 11 Nes W D, Koike K, Jia Z, Sakamoto Y, Satou T, Nikaido T. et al . 9β,19-Cyclosterol analysis by 1H and 13C NMR, crystallographic observations, and molecular mechanics calculations.  J Am Chem Soc. 1998;  120 5970-80
  • 12 Yoshida K, Hirose Y, Imai I, Kondo T. Conformational analysis of cycloartenol, 24-methylenecycloartanol and their derivatives.  Agric Biol Chem. 1989;  53 1901-12
  • 13 Cabrera G M, Seldes A M. Hydroperoxycycloartanes from Tillandsia recurvata. .  J Nat Prod. 1995;  58 1920-4.
  • 14 De-Eknamkul W, Potduang B. Biosynthesis of beta-sitosterol and stigmasterol in Croton sublyratus proceeds via a mixed origin of isoprene units.  Phytochemistry. 2003;  62 389-98
  • 15 Markham K R. Carbon-13 NMR studies of flavonoids-III, naturally occurring flavonoid glycosides and their acylated derivatives.  Tetrahedron. 1978;  34 1389-97
  • 16 Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D. et al . New colorimetric cytotoxicity assay for anticancer-drug screening.  J Nat Cancer Inst. 1990;  4 1107-12.
  • 17 Caviedes L, Delgado J, Gilman R HJ. Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis. .  Clin Microbiol. 2002;  40 1873-4
  • 18 Castillo D, Arevalo J, Herrera F, Ruiz C, Rojas R, Rengifo E. et al . Spirolactone iridoids might be responsible for the antileishmanial activity of a Peruvian traditional remedy made with Himatanthus sucuuba (Apocynaceae).  J Ethnopharmacol. 2007;  112 410-4.
  • 19 Sereno D, Lemesre J L. Axenically cultured amastigote forms as an in vitro model for investigation of antileishmanial agents.  Antimicrob Agents Chemother. 1997;  41 972-6
  • 20 Madureira A M, Spengler G, Molnár A, Varga A, Molnár J, Abreu P M. Effect of cycloartanes on reversal of multidrug resistance and apoptosis induction on mouse lymphoma cells.  Anticancer Res. 2004;  24 859-64

Prof. Dr. Gerald Hammond

Department of Chemistry

University of Louisville

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KY 40292

USA

Phone: +1-502-852-5998

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Email: gb.hammond@louisville.edu