Planta Med 2007; 73(14): 1517-1522
DOI: 10.1055/s-2007-990262
Analytical Methods
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Spectrophotometric Method for Quantitative Determination of Total Anthocyanins and Quality Characteristics of Roselle (Hibiscus sabdariffa)

Tasamaporn Sukwattanasinit1 , Jankana Burana-osot1 , Uthai Sotanaphun1
  • 1Faculty of Pharmacy, Silpakorn University, Nakhon-pathom, Thailand
Further Information

Publication History

Received: April 9, 2007 Revised: August 29, 2007

Accepted: October 5, 2007

Publication Date:
08 November 2007 (online)

Abstract

A simple, rapid and cost-saving method for the determination of total anthocyanins in roselle has been developed. The method was based on pH-differential spectrophotometry. The calibration curve of the major anthocyanin in roselle, delphinidin 3-sambubioside (Dp-3-sam), was constructed by using methyl orange and their correlation factor. The reliability of this developed method was comparable to the direct method using standard Dp-3-sam and the HPLC method. Quality characteristics of roselle produced in Thailand were also reported. Its physical quality met the required specifications. The overall chemical quality was herein surveyed for the first time and it was found to be the important parameter corresponded to the commercial grading of roselle. Total contents of anthocyanins and phenolics were proportional to the antiradical capacity.

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Associate Prof. Dr. Uthai Sotanaphun

Department of Pharmacognosy

Faculty of Pharmacy

Silpakorn University

Mueang

Nakhon-pathom 73000

Thailand

Phone: +66-34-255-800

Fax: +66-34-255-801

Email: uthai@email.pharm.su.ac.th