Resveratrol oligomers from Vitis Vinifera L. stems

Stilbenes an important polyphenolic subclass are widely distributed in the family Vitaceae and Leguminosae, they are reported to decrease the incidence of coronary heart disease [1], reduce platelet aggregation and provide antioxidative and carcinogenic protection [2], moreover, stilbenes are known for their beneficial effect for the plant itself as they are produced in response to fungal infection or abiotic stresses such as heavy metal ions or UV light [3]. In this survey, we report the isolation of trans scirpusin A, a resveratrol dimer for the first time, from stems of Vitis vinifera, along with known stilbenes, trans resveratrol, (+)trans-ε-viniferin, and resveratrol tetramers, hopeaphenol and isohopeaphenol. Centrifugal Partition Chromatography was used for fractionation. Final purification was achieved using semi-preparative HPLC. Identification of the compounds was performed using 1H NMR, 2D correlations and mass spectrometry.

References: [1] A. St Leger et al. (1979) Lancet 1: 1017–1020. [2] E. Frankel et al. (1993) Lancet 341: 454–457. [3] P. Jeandet et al. (1997) Anal. Chem. 69: 5172–5177