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DOI: 10.1055/s-2007-987151
Compounds from Salvia ringens Sibth. et Sm. with cytotoxic activity
The genus Salvia (Lamiaceae) is a rich source of non-volatile compounds (e.g. flavonoids, phenolics, di- and triterpenes) [1]. These components have several beneficial biological effects [2,3,4]. Our previous study revealed a promising cytotoxic activity of Salvia ringens Sibth. et Sm. Literature provides only a few data on this sage species, so it was decided to investigate it for its bioactive compounds. The plant material was collected in Bulgaria (Shumensko Plato National Park). An acetone extract of the roots was subjected to multiple chromatographic separations under the guidance of antiproliferative assay on HeLa cells using MTT assay. From the most active fractions seven abietane diterpenes were isolated by preparative column and thin-layer chromatographic purification. Until now, royleanone, horminone, 7-O-methylhorminone and 7-acetylhorminone were unambiguosly elucidated. The structures were established by ESI-mass spectroscopy and advanced two-dimensional NMR methods, including 1H NMR, JMOD, 1H-1H COSY, NOESY, HSQC and HMBC experiments. All the elucidated compounds were evaluated for cytotoxic activity and displayed marked concentration-dependent effects.
References: [1] Kintzios, S.E. (2000) Sage. The genus Salvia. Harwood Academic Publishers, Amsterdam. [2] Miliauskas, G. et al. (2004) Food Chem. 85: 231–237. [3] Ulubelen, A. (2003) Phytochemistry 64: 395–399. [4] Chen, X. et al. (2002) J. Nat. Prod. 65: 1016–1020.