The Relationship of Serum Lipoprotein Lipase Mass with Fasting Serum Apolipoprotein B-48 and Remnant-like Particle Triglycerides in Type 2 Diabetic Patients

Abstract

Background: There have been no previous reports showing specifically the relation between lipoprotein lipase (LPL) and apolipoprotein (apo) B-48 or remnant metabolism. In this study, we have clarified the relationships of LPL mass in pre-heparin with serum apo B-48 measured by enzyme-linked immunosorbent assay, triglycerides (TG), and remnant-like particle triglycerides (RLP-TG).

Material and Methods: Seventy-nine type 2 diabetic subjects [age, 55±13; body mass index (BMI), 25±5.0 kg/m²; fasting plasma glucose (FPG), 7.39±2.22 mmol/l, HbA1c, 6.5±1.3%, total cholesterol (TC), 5.36±1.09 mmol/l, TG, 2.32±2.53 mmol/l; HDL-C, 1.22±0.44 mmol/l; serum LPL mass, 45±22 ng/ml; apo B-48, 6.6±6.3 μg/ml] were recruited in this study. Fasting serum apo B-48 were measured by ELISA using anti-human apo B-48 monoclonal antibodies (MoAb) and LPL mass by ELISA using anti-bovine milk LPL MoAb. RLP-TG levels were measured using monoclonal antibodies to apo B-100 and apo A-1.

Results: There was no relationship of LPL mass to age, BMI, FPG, and HbA1c. Serum LPL mass was correlated inversely with TG (r=-0.529 p<0.0001) and positively with HDL-C (r=0.576, p<0.0001). Also, LPL mass showed inverse correlations with apo B-48 (r=-0.383 p<0.0001) and RLP-TG (r=-0.422 p<0.0001, n=51). Multiple regression analysis with TG, apo B-48, or RLP-TG as dependent variables, and age, gender, BMI, plasma glucose, and LPL mass as independent variables showed that LPL mass was associated independently with TG, apo B-48, or RLP-TG.

Conclusion: The decrease in LPL protein mass could cause an increase in serum apo B-48 and RLP-TG levels, which is related to the retardation of remnant metabolism.

Key words

Chylomicrons - ELISA - plasma glucose - body mass index - HDL-cholesterol

References

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