α-Glucosidase Inhibitor Proteins from Sesbania grandiflora Flowers

Abstract

Two alpha-glucosidase inhibitors were isolated from the flowers of Sesbania grandiflora and named SGF60 and SGF90. The procedure involved extraction with phosphate buffer, precipitation with ammonium sulfate, ion-exchange chromatography on DEAE-cellulose and gel filtration on Superdex-200. These proteins were identified by using tandem mass spectrometry. The results show partial amino acid sequences of SGF60 similar to p27SJ, a protein from Hypericum perforatum found to suppress HIV-1 gene expression. SGF90 matched a beta-glucosidase from Arabidopsis thaliana.

Key words

Sesbania grandiflora - alpha-glucosidase inhibition - Leguminosae -

References

• 1 Lee S C, Gepts P L, Whitaker J R. Protein structures of common bean (Phaseolus vulgaris) α-amylase inhibitors.  J Agric Food Chem. 2002;  50 6618-27.
• 2 Charles S AD, Paulo A MM, Maura V P, Carlos B J, Octavio L F, Maria F G. Identification of a novel bean α-amylase inhibitor with chitinolytic activity.  FEBS Lett. 2005;  579 5616-20.
• 3 Ye X, Ng T B. A chitinase with antifungal activity from the mung bean.  Protein Express Purif. 2005;  40 230-6.
• 4 Elvira G, Valentin I. Lectins as bioactive plant proteins: a potential in cancer treatment.  Crit Rev Food Sci Nutr. 2005;  45 425-45.
• 5 Wong J H, Ng T B. Isolation and characterization of a glucose/mannose/rhamnose-specific lectin from the knife bean Canavalia gladiata .  Arch Biochem Biophys. 2005;  91-8.
• 6 Ye X Y, Ng T B. Isolation of a novel peroxidase from French bean legumes and first demonstration of antifungal activity of a non-milk peroxidase.  Life Sci. 2002;  71 1667-80.
• 7 Ye X Y, Ng T B, Paul W KT, Wang J. Isolation of a homodimeric lectin with antifungal and antiviral activities from red kidney bean (Phaseolus vulgaris) seeds. J.  Protein Chem. 2001;  20 365-7.
• 8 Cesar A SA, Maria T SC, Luana C BBC, Silene C N, Nereide S SM. Antitumor activity of Cratylia mollis lectin encapsulated into liposomes.  Int J Pharm. 2004;  278 435-45.
• 9 Ye X Y, Ng T B. Isolation of unguilin, a cyclophilin-like protein with anti-mitogenic, antiviral, and antifungal activities, from black-eyed pea.  J Protein Chem. 2001;  20 353-9.
• 10 Wojciechowski M F, Lavin M, Sanderson M J. A phylogeny of legumes (Leguminosae) based on analysis of the plastid matK gene resolves many well-supported subclades within the family.  Am J Bot. 2004;  91 1846-62.
• 11 Bollag D, Rozycki M D, Edelstein S J. Protein methods. 2nd edition Baltimore; John Wiley & Sons Inc 1996.
• 12 Darbinian-Sarkissian N, Darbinyan A, Otte J, Radhakrishnan S, Sawaya B E, Arzumanyan A. et al . p27SJ, a novel protein in St John's Wort, that suppresses expression of HIV-1 genome.  Gene Ther. 2006;  13 288-95.
• 13 Ye X Y, Wang H X, Ng T B. Dolichin, a new chitinase-like antifungal protein isolated from field beans (Dolichos lablab).  Biochem Biophys Res Commun. 2000;  269 155-9.