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DOI: 10.1055/s-2007-981599
© Georg Thieme Verlag KG Stuttgart · New York
α-Glucosidase Inhibitor Proteins from Sesbania grandiflora Flowers
Publication History
Received: April 30, 2007
Revised: July 8, 2007
Accepted: July 19, 2007
Publication Date:
07 September 2007 (online)
![](https://www.thieme-connect.de/media/plantamedica/200711/lookinside/thumbnails/10.1055-s-2007-981599-1.jpg)
Abstract
Two alpha-glucosidase inhibitors were isolated from the flowers of Sesbania grandiflora and named SGF60 and SGF90. The procedure involved extraction with phosphate buffer, precipitation with ammonium sulfate, ion-exchange chromatography on DEAE-cellulose and gel filtration on Superdex-200. These proteins were identified by using tandem mass spectrometry. The results show partial amino acid sequences of SGF60 similar to p27SJ, a protein from Hypericum perforatum found to suppress HIV-1 gene expression. SGF90 matched a beta-glucosidase from Arabidopsis thaliana.
Key words
Sesbania grandiflora - alpha-glucosidase inhibition - Leguminosae -
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Polkit Sangvanich
Department of Chemistry
Faculty of Science
Chulalongkorn University
Bangkok
Thailand
Phone: +66-(2)-218-7637
Fax: +66-(2)-218-7598
Email: spolkit@chula.ac.th