Planta Med 2007; 73(11): 1197-1201
DOI: 10.1055/s-2007-981599
Natural Product Chemistry
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

α-Glucosidase Inhibitor Proteins from Sesbania grandiflora Flowers

Apaporn Boonmee1 , Colin D. Reynolds2 , Polkit Sangvanich1
  • 1Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
  • 2School of Biomolecular Sciences, Liverpool John Moores University, Liverpool, UK
Further Information

Publication History

Received: April 30, 2007 Revised: July 8, 2007

Accepted: July 19, 2007

Publication Date:
07 September 2007 (online)

Abstract

Two alpha-glucosidase inhibitors were isolated from the flowers of Sesbania grandiflora and named SGF60 and SGF90. The procedure involved extraction with phosphate buffer, precipitation with ammonium sulfate, ion-exchange chromatography on DEAE-cellulose and gel filtration on Superdex-200. These proteins were identified by using tandem mass spectrometry. The results show partial amino acid sequences of SGF60 similar to p27SJ, a protein from Hypericum perforatum found to suppress HIV-1 gene expression. SGF90 matched a beta-glucosidase from Arabidopsis thaliana.

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Polkit Sangvanich

Department of Chemistry

Faculty of Science

Chulalongkorn University

Bangkok

Thailand

Phone: +66-(2)-218-7637

Fax: +66-(2)-218-7598

Email: spolkit@chula.ac.th