Bioassay-guided fractionation of the anti-HIV-1 active EtOAc extract from leaves and twigs of Ochna integerrima led to the isolation of five new flavonoid glycosides 1 - 5, five known flavonoids 6 - 10, and two known flavonoid glycosides 11 and 12. Structures were determined based on spectroscopic analyses. 6-γ,γ-Dimethylallyldihydrokaempferol 7-O-β-D-glucoside (1), 6-γ,γ-dimethylallylquercetin 7-O-β-D-glucoside (3), 6-(3-hydroxy-3-methylbutyl)taxifolin 7-O-β-D-glucoside (4), 6-(3-hydroxy-3-methylbutyl)quercetin 7-O-β-D-glucoside (5), and 6-γ,γ-dimethylallyltaxifolin 7-O-β-D-glucoside (11) showed anti-HIV-1 activities in the syncytium assay using the ΔTat/revMC99 virus and the 1A2 cell line system with EC50 values ranging from 14.0 - 102.4 μg/mL. Furthermore, ochnaflavone 7″-O-methyl ether (7) and 2″, 3″-dihydroochnaflavone 7″-O-methyl ether (8) were very active; they exerted activities in the syncytium assay with EC50 values of 2.0 and 0.9 μg/mL, respectively, and likewise inhibited HIV-1 reverse transcriptase (RT) with IC50 values of 2.0 and 2.4 μg/mL, respectively.
3
Messanga B B, Tih R G, Kimbu S F, Sondengam B L, Martin M T, Bodo B.
Calodenone, a new isobiflavonoid from Ochna calodendron
.
J Nat Prod.
1992;
55
245-8.
11
Ichino C, Kiyohara H, Soonthorncharoennon N, Chaukul W, Ishiyama A, Sekiguchi H. et al .
Antimalarial activity of biflavonoids from Ochna integerrima
.
Planta Med.
2006;
72
611-4.
12
Rukachaisirikul V, Pailee P, Hiranrat A, Tuchinda P, Yoosook C, Kasisit J. et al .
Anti-HIV-1 protostanes and digeranylbenzophenone from trunk bark and stems of Garcinia speciosa
.
Planta Med.
2003;
69
1141-6.
13
Tuchinda P, Saiai A, Pohmakotr M, Yoosook C, Kasisit J, Napaswat C. et al .
Anti-HIV-1 cycloartanes from leaves and twigs of Gardenia thailandica
.
Planta Med.
2004;
70
366-70.
17
Tan T G, Pezzuto J M, Kinghorn A D, Hughes S H.
Evaluation of natural products as inhibitors of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase.
J Nat Prod.
1991;
54
143-54.
18
Nara P L, Hatch W C, Dunlop N M, Robey W G, Arthur L O, Gonda M A. et al .
Simple, rapid, quantitative, syncytium forming microassay for the detection of human immunodeficiency virus neutralizing antibody.
AIDS Res Human Retrovir.
1987;
3
283-302.
19
Kiser R, Makovsky S, Terpening S J, Laing N, Clanton D J.
Assessment of a cytoprotection assay for the discovery and evaluation of anti-human immunodeficiency virus compounds utilizing a genetically-impaired virus.
J Virol Meth.
1996;
58
99-109.
20
Chou T -C, Talalay P.
Quantitative analysis of dose-effect relationships: The combined effects of multiple drugs or enzyme inhibitors.
Adv Enzyme Regul.
1984;
22
27-55.
22
Wu T S, Hsu M Y, Kuo P C, Sreenivasulu B, Damu A G, Su C R. et al .
Constituents from the leaves of Phellodendron smurensa var. wilsonii and their bioactivity.
J Nat Prod.
2003;
66
1207-11.
25
Calis I, Heilmann J, Tasdemir D, Linden A, Ireland C M, Sticher O.
Flavonoid, iridoid and lignan glycosides from Putoria calabrica
.
J Nat Prod.
2001;
64
961-4.
29
McKee T C, Bokesch H R, McCormick J L, Rashid M A, Spielvogel D, Gustafson K R. et al .
Isolation and characterization of new anti-HIV and cytotoxic leads from plants, marine and microbial organisms.
J Nat Prod.
1997;
60
431-8.