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DOI: 10.1055/s-2007-979980
© Georg Thieme Verlag Stuttgart · New York
Inhibitory Effect of Thyrotropin-Releasing Hormone on Enzyme Secretion from Isolated Rat Pancreatic Acinar Cells
Publication History
1993
1995
Publication Date:
23 April 2007 (online)
Abstract
This study reports a direct effect of TRH on amylase secretion from isolated rat exocrine pancreatic acinar cells. TRH inhibited carbachol (10-5 M)-stimulated amylase secretion by a maximum of 24% at a concentration of 10-11 M (p < 0.05), but did not affect basal amylase release in concentrations from 10-13 M to 10-8 M. Ceruletide (3 × 10-10 M)-stimulated amylase secretion was maximally reduced by 23% at a TRH concentration of 10-10 M (p < 0.05). Direct stimulation of proteinkinase C-mediated secretion by the diacylglycerol analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was not altered by TRH. The TRH metabolite cyclo (His-Pro) did not influence basal or stimulated pancreatic secretion in vitro. These findings point to a TRH-mediated modulation of exocrine pancreatic secretion at the receptor site.
Key words
Thyrotropin-Releasing Hormone(TRH) - Histidyl-Proline Diketopiperazine [Cyclo (His-Pro)] - Exocrine Pancreas - Pancreatic Acinar Cells - Enzyme Secretion