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Horm Metab Res 1995; 27(8): 367-371
DOI: 10.1055/s-2007-979980
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© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effect of Thyrotropin-Releasing Hormone on Enzyme Secretion from Isolated Rat Pancreatic Acinar Cells

T. P. Kemmer1 , L. Duntas2 , W. Munz2 , P. Malfertheiner3
  • 1Rehabilitationsklinik Neresheim, Neresheim, Germany
  • 2Abteilung Innere Medizin I, Universitätsklinik Ulm, Ulm, Germany
  • 3Klinik für Gastroenterologie und Hepatologie, Otto-von-Guericke-Universität Magdeburg, Magdeburg, Germany
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Publication History

1993

1995

Publication Date:
23 April 2007 (online)

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Abstract

This study reports a direct effect of TRH on amylase secretion from isolated rat exocrine pancreatic acinar cells. TRH inhibited carbachol (10-5 M)-stimulated amylase secretion by a maximum of 24% at a concentration of 10-11 M (p < 0.05), but did not affect basal amylase release in concentrations from 10-13 M to 10-8 M. Ceruletide (3 × 10-10 M)-stimulated amylase secretion was maximally reduced by 23% at a TRH concentration of 10-10 M (p < 0.05). Direct stimulation of proteinkinase C-mediated secretion by the diacylglycerol analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was not altered by TRH. The TRH metabolite cyclo (His-Pro) did not influence basal or stimulated pancreatic secretion in vitro. These findings point to a TRH-mediated modulation of exocrine pancreatic secretion at the receptor site.

Key words

Thyrotropin-Releasing Hormone(TRH) - Histidyl-Proline Diketopiperazine [Cyclo (His-Pro)] - Exocrine Pancreas - Pancreatic Acinar Cells - Enzyme Secretion