Horm Metab Res 2000; 32(11/12): 509-514
DOI: 10.1055/s-2007-978678
Original

© Georg Thieme Verlag Stuttgart · New York

Beta-Adrenoceptor Agonists and Other cAMP Elevating Agents Suppress PAI-1 Production of Human Adipocytes in Primary Culture

H. Gottsching-Zeller, I. Aprath, T. Skurk, H. Hauner
  • German Diabetes Research Institute at the Heinrich-Heine-University Düsseldorf, Clinical Department, Düsseldorf, Germany
Further Information

Publication History

2000

2000

Publication Date:
19 April 2007 (online)

Recent studies showed that catecholamines contribute to the regulation of plasminogen activator inhibitor-1 (PAI-1) expression, at least in endothelial cells. Aim of this study was to examine the role of catecholamines on PAI-1 production by human adipocytes and, in particular, to clarify which adrenoceptor (AR) subtypes are involved. Addition of the unselective AR agonist isoproterenol led to a dose- and time-dependent suppression of PAI-1 mRNA and protein release in adipocytes from the subcutaneous and omental depot of obese subjects. A similar degree of suppression was observed in subcutaneous mammary adipocytes of lean women. This effect was mainly mediated via the β2-adrenoceptor according to experiments using selective agonists. Moreover, addition of cAMP-elevating agents such as dibutyryl-cAMP, forskolin and the phosphodiesterase inhibitors isobutyl-methylxanthine and milrinone resulted in a reduction of PAI-1 of varying degrees. In conclusion, the results of this study support the assumption that catecholamines are able to down-regulate PAI-1 expression and secretion in human adipocytes via β-adrenergic receptors.